SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Lun Han,Xu Wang,Bin Yu,Xiaoyuan Qin,Baocheng Liu,Xu Han,Huifen Yuan,Bin Yu,Zhiqi Zhao
Glucose is a key biomarker of diabetes, and effective glucose monitoring methods are crucial to the prevention and management of diabetes. Therefore, in this paper, Fe 3 O 4 /DEX/PDA@Au (Raman reporters) @Au nanocomposites were synthetized that with DTNB (5,5′-dithiobis(2-nitrobenzoic)), MMTA (2-mercapto-4-methyl-5-thiazole acetic acid), MBA (4-mercaptobenzoic acid) and 4-Mpy(4-Mercaptopyridine) were used separately as Raman reporters. Fe 3 O 4 and PDA (Polymerized dopamine) could supply more high surface area of active sites and high SERS (Surface-Enhanced Raman Scattering) substrate, which has high stability and reproducibility. Dextran coating is an effective way to prepare biocompatible materials TEM, XRD, TG and VSM were used to analyze the size, morphology and magnetic properties of the nanocomposites. Fe 3 O 4 /DEX/PDA@Au(Raman reporters)@Au that integrates a multi-hotspot structure and magnetic separation techniques were studied the enhancement effect of Raman spectra, and glucose solutions with different concentrations were tested. Furthermore, the optimal Fe 3 O 4 /DEX/PDA@Au(Raman reporters)@Au nanocomposites were supplied as SERS substrates for detection of glucose accurately and quickly in sweat. SERS signal intensity is linearly correlated with glucose concentration within the measurement range of 5 × 10 -3 to 10 mM, and the minimum detectable concentration is 5 µM. The Fe 3 O 4 /DEX/PDA@Au(Raman reporters)@Au nanocomposites exhibit high reliability, specificity and repeatability of the strategy were then verified by practical detection of sweat.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Ying Zhang,Qian Liu,Mengting Liu,Xueni Zhang,Xiaoya Li,Lihui Dai,Meng Meng,Dingbin Liu,Yongmei Yin,Rimo Xi
The microenvironment of lysosomes plays an important role in various functions within cells. Abnormal pH changes in lysosomes account for multiple diseases including tumors. Acid-responsive nanomaterials with reconfigurable structures and properties have garnered significant interests. Owing to the potential in biosensing, controlled release and nanomachinery, DNA based pH-responsiveness has been widely pursued. Herein, we designed an AuNPs-i-motifs based SERS probe to monitor the pH dynamics of lysosomal maturation in different cells. Taking advantages of biocompatibility and structural flexibility of DNA, unique optical and plasmonic properties of gold nanoparticles , excellent stability and enhanced sensitivity of background-free Raman reporter, the designed SERS probe could indicate the pH variation dynamically in lysosomes. What is more valuable is that 4-mercaptobenzonitrile is not only used as a Raman reporter to provide sensitive Raman signal for pH sensing, but also used as a sealing agent allowing for controllable aggregation, to improve the stability and accuracy of the sensor. With this versatile design, the sensor was prepared simply and feasibly and a tedious trial was avoided. It provides a feasible tool for understanding the relationship of lysosomal pH with fundamental cellular functions and mechanism of diseases.
研究领域:
生物传感
·
纳米医学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL SCIENCES
·
2020
·Vol. 36
·期 9
·1025-1030
·
IF 2.049
Xiaoxuan LI,Lijun LI,Yuhan HE
Based on the reversible covalent binding between borates and glycoproteins, we proposed a scheme that can specifically recognize and detect immunoglobulin G (IgG). The application of magnetic materials to enrich SERS probes can provide an enhanced signal and repeatability. The results showed that the prepared sandwich-like complex displayed high sensitivity and excellent selectivity for IgG. The Raman peak intensity showed a good linear relationship in the concentration range of 1 mg/mL – 1 ng/mL. The linear equation was y = 657.93x + 2963.9, R2 = 0.996. Parallel testing of this sandwich-like complex proved to have excellent repeatability. This method provided a new possibility for clinical non-immunity and the quantitative detection of IgG.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2022
·
IF 9.965
Aodi Zhang,Yusai Zhou,Feng Xue,Xudong Peng,Hongyu Chen,Hong Wang
The surface enhanced Raman scattering (SERS) reporters are rather limited, and the Raman peaks still overlap in varying degrees, making SERS multiplex coding a critical bottleneck in the exploration of SERS nanotechnology. Herein, we design a general strategy to expand the SERS probe scope to 26 probes of six types, which can be further expanded within a limited range, with stable performance and structure. By constructing (Au-aggregate)@Ag@silica and (Au-aggregate)@silica nanocomposites, we develop optimal enhancement strategies for each Raman molecules. Mixed signal-ligand SERS probes improve the complexity of Raman spectra and expand the coding capacity. By integrating the strategies, SERS inks are produced and applied in anti-counterfeiting. With these improvements, this work breaks the constrains of probe selection, bringing SERS one step closer to the sensor or anti-counterfeiting application.
研究领域:
纳米技术
·
材料科学
·
分析化学
·
光学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Pan Tao,Yang Luo,Kai Zheng,Jing Cao,Li Zhu,Xunsi Wang,Wei Zhang,Shixun Dai,Qiushun Zou,Peiqing Zhang
Background Permethrin is a pesticide used to kill insects, and once used in excess, it poses a great threat to the environment and human health, therefore, it is necessary to realize the rapid and accurate detection of permethrin. Fiber optic surface enhanced Raman scattering (SERS) probes have the advantages of small volume and can be used for remote monitoring, which have great potential for application in achieving in-situ detection of pesticide residues. Results Fiber taper waist (FTW) SERS probes modified by silver nanocubes-graphene oxide (Ag NCs-GO) composite structures were prepared for in situ detection of permethrin in lake water. Evanescent wave was used to excite SERS signals from Ag NCs-GO composite substrates, which was designed to enhance signals through expanded excitation light-analyte contact area. Ag NCs can provide stronger hot spots, and GO further enhances the sensitivity of SERS through electron transfer and improves the stability of the detection. The detection limit of prepared FTW SERS probe for Rhodamine 6G (R6G) was calculated to be 8.5×10 -9 M and RSD were all below 5% using the drop-casting method. The in-situ detection limit of R6G and permethrin in water was tested to be 10 -6 M and 10 -5 M, respectively. Significance This work utilizes fiber optic evanescent waves to excite SERS signals, and adopts a special FTW structure and SERS substrates to achieve good performance. The synergistic enhancement of electromagnetism and chemistry is realized while increasing the contact area, which improves the sensitivity of in situ detection of the pesticide residue permethrin. It Promotes the application of fiber optic SERS sensors in pesticide residue detection.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2015
·Vol. 21
·期 18
·6817-6822
·
IF 5.731
Xuemei Li,Linlin Wang,Chunxiang Li
Graphical Tagging along : An ultrasensitive surface-enhanced Raman spectroscopy (SERS) sensor based on rolling-circle amplification (RCA)-increased “hot-spot” is developed for the detection of thrombin, with core-shell nanoparticles (see figure; RBITC=Rhodamine B isothiocyanate, MB=magnetic beads) as Raman reporter. An ultrasensitive surface-enhanced Raman spectroscopy (SERS) sensor based on rolling-circle amplification (RCA)-increased “hot-spot” was developed for the detection of thrombin. The sensor contains a SERS gold nanoparticle@Raman label@SiO 2 core-shell nanoparticle probe in which the Raman reporter molecules are sandwiched between a gold nanoparticle core and a thin silica shell by a layer-by-layer method. Thrombin aptamer sequences were immobilized onto the magnetic beads (MBs) through hybridization with their complementary strand. In the presence of thrombin, the aptamer sequence was released; this allowed the remaining single-stranded DNA (ssDNA) to act as primer and initiate in situ RCA reaction to produce long ssDNAs. Then, a large number of SERS probes were attached on the long ssDNA templates, causing thousands of SERS probes to be involved in each biomolecular recognition event. This SERS method achieved the detection of thrombin in the range from 1.0×10 −12 to 1.0×10 −8 M and a detection limit of 4.2×10 −13 M , and showed good performance in real serum samples.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
分子诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Qiang Zou,Xinchen Ai,Tao Xue
In this work, an environment-friendly and label-free SERS probe was synthesized through Tollens' reagent and phytic acid to detect Fe 3+ ions in the integrated circuit cleaning solution waste. The synthesis process was greatly shortened to 30 min compared with the traditional synthesis time of 6 h. In addition, this SERS probe can be directly used and avoid the environmental pollution caused by the further modification in the synthesis process. This probe has a high sensitivity (LOD = 0.1 μmol L −1 ) and a wide detection range from 0.5 μmol L −1 to 500 μmol L −1 .As a practical application, it has been successfully applied to detect Fe 3+ ions in the standard clean two solution (SC-2). This method provides a feasible detection way for whether integrated circuit cleaning solution waste meets the emission criteria.
研究领域:
环境科学
·
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Haozhi Lei,Haoze Wang,Xiqiu Wang,Zeyu Xiao,Tian Tian,Kai Cui
Detection of progression is of great importance to breast cancer treatment and can benefit patients. Limited by current detection technologies and biomarkers, early breast cancer progression diagnosis remains challenging. Researchers have found blood extracellular vesicles (EVs)-derived integrin α6β4 directly facilitate progression in breast cancer, enabling cancer detection. However, EVs size and heterogeneity hinder protein detection, masked by abundant background EVs. Hence, novel tools for efficient detection of EVs with high selectivity and low interference are significantly desired. Here, a new silver-coated gold nanorods SERS probe, termed as Au@Ag@IDA-B/4MSTP, based on DNA aptamer was established for the detection of integrin α6β4 derived from EVs. Validation of the Au@Ag@IDA-B/4MSTP probes using cell-culture-derived EVs revealed a LOD of 23 particles/μL for EVs detection. This tool was further confirmed to mimic the real state of cancer with subcutaneous tumor model and lung metastasis model in mice. With 10 μL of blood plasma and simple Raman analysis process, the test achieved 85.7 % sensitivity and 83.3 % specificity. Moreover, our method achieves a simplified approach that expedites the detection process. These results demonstrate the good detection performance of Au@Ag@IDA-B/4MSTP probes for EVs integrin α6β4, and suggest that this non-invasive approach could be a promising tool for early detection of breast cancer progression.
研究领域:
癌症研究
·
医学诊断
·
纳米技术
·
生物标志物检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 11
·3582
·
IF 4.927
Yinping Zhao,Yawei Kong,Liwen Chen,Han Sheng,Yiyan Fei,Lan Mi,Bei Li,Jiong Ma
With the development of precision medicine, antigen/antibody-targeted therapy has brought great hope to tumor patients; however, the migration of tumor cells, especially a small number of cells flowing into blood or other tissues, remains a clinical challenge. In particular, it is difficult to use functional gold nanomaterials for targeted clinical tumor diagnosis while simultaneously obtaining stable and highly sensitive Raman signals. Therefore, we developed a detection method for functional Au Nanostars (AuNSs) with dual signal enhancement that can specifically track location and obtain high-intensity surface-enhanced Raman scattering (SERS) signals. First, AuNSs with specific optical properties were synthesized and functionalized. The Raman dye 4-mercapto-hydroxybenzoic acid and polyethylene glycol were coupled with the tumor marker, epidermal growth factor receptor, to obtain the targeted SERS probes. In addition, a detection chip was prepared for Raman detection with physical enhancement, exhibiting a 40-times higher signal intensity than that of quartz glass. This study combines physical enhancement and SERS enhancement technologies to achieve dual enhancement, enabling the detection of a highly sensitive and stable Raman signal; this has potential clinical value for antigen/antibody-targeted tumor diagnosis and treatment.Keywords:SERS probe;dual enhancement;stable and highly sensitive;Raman signals;A549 cell
研究领域:
纳米技术
·
生物医学工程
·
癌症研究
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2022
·
IF 4.177
Minling Li,Dechan Lu,Ruiyun You,Huiying Shen,Lanjin Zhu,Qingqiang Lin,Yudong Lu
Tyrosinase (TYR) is a key rate-limiting enzyme of melanin and a biomarker for melanoma diagnosis. Therefore, detection of TYR in biological samples is essential for the clinical diagnosis of melanoma. A novel portable surface-enhanced Raman scattering (SERS) sensor is proposed to detect TYR activity. It is based on dopamine (DA)-functionalized Au nanorod (Au NR) arrays as the capture substrate and 4-mercaptophenylboronic acid (4-MPBA)-modified silver nanoparticles (Ag NPs) as the SERS probe. In this case, the Au NR arrays are modified with 3,3′-dithiodipropionic acid di(N-succinimide ester) (DSP) to capture DA. In the presence of TYR, DA is oxidized to dopaquinone (DQ), which resulted in a decrease in the concentration of SERS probes further adsorbed onto the arrays. The activity of TYR is detected by changes in the intensity of the phenylboronic acid signal. Furthermore, this detection system has a wide linear detection range and high sensitivity that can effortlessly evaluate a linear range of 0.0001–100 U/mL of TYR activity. More significantly, the presented SERS sensor can perform well on both the determination of TYR activity in serum and the assessment of TYR inhibitors, which offers new opportunities for diagnosis of melanoma and other TYR-related diseases under complex biological conditions.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
·
癌症诊断
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Ruimin Li,Yuting Zhang,Jing Tan,Jiaxun Wan,Jia Guo,Changchun Wang
Utilizing fluorescence reporters and SERS probes as the security labels, a series of dual-mode encoded magnetic composite microspheres with micrometer size was designed and prepared for anticounterfeiting applications. At first, the micro-meter-sized melamine formaldehyde microspheres with different fluorescence molecules (FMF) were prepared by precipitation polymerization, and then the magnetite composite microspheres (FMF/MNPs) were fabricated by direct immobilization of magnetic nanoparticles (MNPs) onto the surface of FMF microspheres. After deposition of Ag nanoparticles (Ag-NPs) onto FMF/MNPs microspheres, the SERS probes were absorbed onto the surface of Ag-NPs, and then a protection layer of silica was coated on the composite microspheres by Stöber method. The combination of different fluorescence reporters and SERS probes greatly increased the encoding complexity and volume for high-level anticounterfeiting. The structure of the dual-encoded FMF/MNPs/Ag-NPs/SiO2 composite microspheres was characterized by FESEM, TEM, FLS(fluorescence spectrometer), XRD, VSM, UV–vis and EDS. The embedded magnetic nanoparticles enable the composite microspheres to be quickly isolated from the marked latex paint by magnet at the concentration of as low as 1 ppm, and the covert tag information can be read out even from one composite microsphere. In addition, the covert security information in the marked coating film can be also read out in situ and the existence of the composite microspheres does not influence the visible appearance of the coating film. All the above outstanding properties will make these dual-mode encoded composite microspheres as advanced security tags for next-generation anticounterfeiting applications.
研究领域:
材料科学
·
纳米技术
·
分析化学
·
安全与法医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）