Advanced Science
·
2024
·
IF 14.3
Guanyu Huang,Jiajun Zhang,Yu Xu,Fei Wu,Yiwei Fu,Xuelin Zhang,Hanxiao Yin,Yuanyuan You,Peng Zhao,Weihai Liu,Jingnan Shen,Junqiang Yin
LACTB is identified as a tumor suppressor in several tumors. However, preliminary study reveals that LACTB is overexpressed in osteosarcoma and indicates poor prognosis. Two missense mutations (rs34317102 and rs2729835) exist simultaneously in 92.31% of osteosarcoma patients and cause M5L and R469K double mutations in LACTB, suggesting the biologic function of LACTB protein may be altered in osteosarcoma. Moreover, LACTB M5L+R469K overexpression can promote malignant progression in different tumors, which suggests that the M5L and R469K mutations confer oncogene-like functions to LACTB. Mechanistically, LACTB M5L+R469K not only reduces the wild type p53 via enhancing PSMB7 catalytic activity, but also protects p53 R156P protein from lysosomal degradation, which suggesting LACTB M5L+R469K is a dual-regulator for wt-p53 and mutant p53, and derive oncogene-like functions. More importantly, clavulanate potassium, a bacterial β -lactamase inhibitor, can inhibit osteosarcoma proliferation and sensitize osteosarcoma to cisplatin by binding and blocking LACTB M5L+R469K . These findings revealed that the M5L and R469K double mutations can diminish the tumor suppressive ability of wild type LACTB and provide oncogene-like functions to LACTB. Inhibiting LACTB M5L+R469K can suppress the progression of osteosarcoma harbouring wild-type or mutant p53. Clavulanate potassium is a promising drug by targeting LACTB M5L+R469K -p53 pathway for the treatment of osteosarcoma patients.
研究领域:
肿瘤学
·
遗传学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Chemistry Frontiers
·
2025
·
IF 6.4
Qing Li,Weiping Guo,Binqiang Chai,Kang Xu,Weijiang Hu,Xueming Su,Huaixin Wei,Hongbo Geng,Jun Yang,Zhong-Zhen Luo
An innovative SnPS 3 @carbon nanofibers (CNFs) composite anode was successfully synthesized through an integrated electrospinning and thermal phosphosulfuration strategy. Comprehensive structural characterization confirms the uniform dispersion of Sn, P, and S elements within a nitrogen-doped porous carbon matrix, forming an interconnected three-dimensional conductive network. This architecturally optimized design significantly enhances electrical conductivity while providing abundant accessible active sites for efficient sodium ion adsorption and storage.Electrochemical evaluation demonstrates exceptional sodium storage performance: the SnPS 3 @CNFs anode delivers a remarkable reversible capacity of 448.14 mA h g⁻¹ after 980 cycles at an ultrahigh current density of 10 A g⁻¹, representing an enhancement over conventional SnS₂@CNFs and Sn₄P₃@CNFs counterparts.Furthermore, full-cell configuration with a Na₃V₂(PO₄)₃ (NVP) cathode maintains a high reversible capacity of 196.3 mA h g⁻¹ following 200 cycles at 1 A g⁻¹, confirming excellent practical compatibility. The synergistic integration of outstanding cyclability, superior rate capability, and structural integrity establishes this composite as a promising high-performance anode material for next-generation sodium-ion batteries, particularly suited for high-power energy storage applications.
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Chen Lu,Jiayue Chen,Shengsheng Zhang,Yaru Tian,Xinyu Ying,Dan Zhang,Zhidan Luo,Xinxin Si,Ming Li
Two single nucleotide polymorphisms (SNPs) in the human SLCO1B1 gene, c.388A>G (rs2306283) and c.521T>C (rs4149056), are independent determinants of the efficacy and side effects of statin drugs. Multinational clinical guidelines recommend testing for SLCO1B1 genotypes before the initial use of statins. Current SLCO1B1 SNP identification methods, primarily based on quantitative fluorescence PCR, rely on expensive equipment, are time-consuming, and require cold-chain storage for reagents, making them unsuitable for use in resource-limited healthcare settings. In this study, we developed a CRISPR/Cas12b-based amplification-free genotyping technique for SLCO1B1 SNPs. Within 30 minutes of the isothermal reaction, genotyping of the c.388A>G and c.521T>C SNPs in the SLCO1B1 gene can be observed by the naked eyes under blue light. Additionally, maltodextrin was identified as an effective vitrification stabilizer for the CRISPR/Cas12b premix. A low-cost vitrification process was optimized to prepare a glass like solid reagent via room-temperature vacuum drying. The vitrified CRISPR/Cas12b reagent retained approximately 88% of its activity after 30 days of storage at 37 °C, eliminating the need for cold-chain storage and allowing for long-term preservation at room temperature. This vitrified CRISPR/Cas12b based rapid SNP detection technique is especially suitable for genotyping drug metabolism genes in primary healthcare settings, providing effective guidance for precision medicine in clinical practice.
研究领域:
分子生物学
·
医学遗传学
·
精准医疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemical Engineering and Processing-Process Intensification
·
2018
·
IF 2.826
Ting Shu,Nan Hu,Yanyan Xu,Na Li,Zhaoliang Wu,Wei Liu,Chunyan Yang,Ruipeng Di,Ke Lu
Effective recovery of antibiotic from their wastewater is an important but challenging work. Here, a promising process of foam separation had been validated for the recovery of streptomycin sulfate (SS) from its wastewater and the SS concentration in wastewater was 110.0 ± 2.8 mg/L. Firstly, thiol functionalized silica nanoparticles (t-SNPs) were prepared to enhance their adsorption capacity, and they acted as the collectors to capture SS. Moreover, a novel foam separation column containing a polyethylene tube was developed to strengthen foam drainage. A low liquid holdup of the foam was achieved because the polyethylene tube contributed to accelerating the flow of entrained liquid in the exterior channels. Under the suitable conditions of pH 7.0, t-SNPs concentration 500 mg/L and volumetric air flow rate 700 mL/min, the recovery percentage and enrichment ratio of SS reached 90.1 ± 4.5% and 82.4 ± 4.1, respectively. Furthermore, desorption experiments were performed for isolating SS from the t-SNPs and the desorption percentage of SS reached 91.3 ± 4.6% after four cycles. Overall, this work had a significant meaning for the treatment of antibiotic-contaminated wastewater.
研究领域:
环境工程
·
纳米技术
·
化学工程
·
水处理技术
✓ 已验证
DOI
高影响因子
Biomaterials Science
·
2020
·Vol. 8
·期 17
·4757-4766
·
IF 6.183
Jing Zhang,Dan Liu,Jiale Liu,Yanfeng Han,Haiyan Xu,Xigang Leng,Deling Kong,Lanxia Liu
Combined blockades of CTLA-4 and PD-1 can yield better overall complementary clinical outcomes than individual blockades, but the response rates are still relatively low. To investigate the anti-tumor effects of various combined strategies, we designed various spherical nucleotide nanoparticles (SNPs) loaded with CTLA-4 aptamer (cSNPs), PD-1 siRNA (pSNPs) or both (hybrid SNPs, or hSNPs). The results demonstrated that hSNPs could promote significantly stronger anti-tumor immune responses in a nonredundant fashion than the mixture of pSNPs and cSNPs (pSNPs & cSNPs). We reasoned that this is because all individual immune cells could receive both CTLA-4 and PD-1 blockades when they engulfed hSNPs, but it is much less likely that individual immune cells could receive both CTLA-4 and PD-1 blockades as many of them may not take both pSNPs and cSNPS from pSNPs & cSNPs. Further results revealed that the synergistic immune stimulatory effects of CTLA-4 and PD-1 blockades in the form of hSNPs were at least partly through regulating the immune suppressive function of both Tregs and TIM3+ exhausted-like CD8 T cells and allowing effector T cells to expand. This mechanism is not identical to earlier reported mechanisms of CTLA-4 and PD-1 blockades.
研究领域:
癌症免疫治疗
·
纳米技术
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Catalysis Science & Technology
·
2022
·Vol. 12
·期 14
·4486-4497
·
IF 6.177
Si Liu,Zhenfu Wang,Kun Chen,Linling Yu,Qinghong Shi,Xiaoyan Dong,Yan Sun
Chiral amines are valuable intermediates widely applied in the pharmaceutical industry. As an alternative to classical synthetic methods for the production of chiral amines, biocatalytic cascade synthesis for these molecules from their alcohol precursors has been developed. However, currently available enzyme cascade systems remain unstable and unsustainable in the practice of chiral amine synthesis. Herein, the catalytic cascade system of chimeric amine dehydrogenase (AmDH) and alcohol dehydrogenase (ADH) fused with silica binding peptide (SiBP) was site-specifically co-immobilized on silica nanoparticles (SNPs). SiBP enabled the fusion enzymes to firmly bind to SNPs, and both the single immobilized enzymes showed higher catalytic efficiencies (kcat/Km) than their free counterparts to different degrees. Using 10 mM (S)-2-hexanol as the substrate, the co-immobilized enzyme system (SiBP–ADH&SiBP–AmDH@SNPs) showed 90% (R)-2-aminohexane yield after 48 h reaction, which was 1.85-fold higher than the free enzyme system due to the enhanced catalytic efficiency of the immobilized enzymes and the possible proximity effect of the SiBP–ADH&SiBP–AmDH@SNPs. The catalyst productivity of the SiBP–ADH&SiBP–AmDH@SNPs was 11 to 34 times higher than literature data, demonstrating the high economic benefits and catalytic efficiency of this system in producing chiral amines. SiBP–ADH&SiBP–AmDH@SNPs retained 87% of its original activity after eight cycles of reuse. These results highlighted the potential of the co-immobilization system for the effective and sustainable synthesis of chiral amines in industrial practice.
研究领域:
生物催化
·
酶工程
·
药物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ELECTROANALYSIS
·
2021
·Vol. 34
·期 2
·294-301
·
IF 3.223
Chang Wang,Jingyuan Yu,Lijuan Qi,Jiaxue Yu,Meiting Yang,Yan Du
We realized the user-friendly and field-based point-of-care detection on a self-made portable device with the aid of personal glucose meter (PGM) for single nucleotide polymorphisms (SNPs) of melanoma and colon cancer marker BRAF V600E with good sensitivity and selectivity by integrating the loop-mediated isothermal amplification (LAMP) reaction and the strand exchange signal transduction. The device is equipped with a rotating magnetic field as well as a heating element, under which the pre-added magnetic nanochains (MNCs) help to homogenize the reaction mixture and overcome the low diffusion efficacy of the reactant and the required temperature for different reactions can be met.
研究领域:
生物技术
·
医疗器械
·
癌症研究
·
纳米技术
·
分子诊断
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2015
·Vol. 39
·期 6
·4568-4574
·
IF 3.086
Shuchao Hu,Ling Sun,Mingxing Liu,Hongda Zhu,Huiling Guo,Hongmei Sun,Honghao Sun
For accurate determination of local intracellular pH which could further improve our understanding of cellular processes and knowledge of advanced drug delivery system architecture, a fluorescent-based ratiometric nanosensor has been designed over the past decade. As a promising matrix for nanosensors and nanomedicines, silica nanoparticles (SNPs) have been widely used due to their desirable properties, which include a high loading level of guest molecules, low toxicity and easy functionalization. Amination of SNPs was essential for binding functional groups, such as fluorescent molecules and targeting groups, whereas positively charged amine will cause the aggregation of SNPs which limits the further application of SNPs. To circumvent this problem, a simple and effective strategy has been presented in this paper. Firstly, an acid was used as a catalyst instead of a base to inhibit SNP aggregation in the amination process. Subsequently, polymers (poly(ethylene glycol) and hyaluronic acid) were coated on the surface of monodispersed aminated SNPs to obtain excellent colloidal stability. The well dispersed core–shell SNPs were further functionalized with two pH sensitive fluorophores (fluorescein isothiocyanate and Oregon Green isothiocyanate) and one reference fluorophore (rhodamine B isothiocyanate), which resulted in ratiometric pH nanosensors with a hydrodynamic diameter of 76–100 nm. The sensors exhibited a broad pH measurement range from 3.8 to 7.4, which covers almost all intracellular pH values, and remarkable colloidal stability in buffer solution.
研究领域:
纳米技术
·
生物传感器
·
材料科学
·
药物传递系统
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
ENERGY & FUELS
·
2024
·
IF 5.2
Jun Yang,Guancheng Jiang,Shengming Huang,Jingtian Yi,Tengfei Dong,Yinbo He,Lili Yang,Qi Feng,Ge Wang
In this study, a nanobiocatalyst [α-amylase/γ-aminopropyltriethoxysilane-modified silica nanoparticles (A-SNPs)] was prepared for efficient mudcake removal and reservoir damage control during horizontal drilling and openhole completion. The molecular structure, thermal stability, and colloidal properties of the nanobiocatalyst were characterized by using FTIR, TGA, particle size, zeta potential, SEM, and TEM tests. The results confirmed that A-SNPs were securely encapsulated on the surface of α-amylase via covalent imine bonding (C═N), significantly enhancing the stability of α-amylase. Mudcake and polymer plugging removal experiments demonstrated that soaking mudcake in a 1% nanobiocatalyst solution for 4 h reduced the filtrate return time to under 10 min. Additionally, polymer removal efficiency exceeded 63.17% at 500 psi. Core flow experiments combined with NMR analysis revealed that the nanobiocatalyst effectively mitigated permeability damage at 95 °C, achieving a core permeability restoration value of 82.88%. The nanobiocatalyst significantly reduced the fluid flow resistance and improved hydrocarbon availability within the reservoir porous medium. In conclusion, nanobiocatalysts have great potential for application in oil and gas drilling and hydrocarbon recovery.
研究领域:
石油工程
·
纳米技术
·
生物催化
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）