NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 36
·14220-14223
·
IF 3.069
Kai-Xin Xie,Shu-Shu Jia,Jin-Hua Zhang,Huan Wang,Qiang Wang
The enhancement of surface plasmon-coupled emission (SPCE) by CNT assistance was investigated with the factor of 12 and 65 as compared to normal SPCE and free space emission, respectively. This strategy designed as an immunosensor to detect IgG with a detection limit of 0.0025 ng mL−1 provides a universal way to improve the detection sensitivity in fluorescence-based sensing platforms.
研究领域:
纳米技术
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光学
·
分析化学
·
生物传感器
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Hui Liu,Yi-Wei Liu,Ruo-Yu Yang,Mei-Juan Wu,Zi-Wei Yu,Jing-Wen Han,Chen-Zhi Zhang,Pin-Fang Huang,Ai-Lin Liu,Meng-Meng Liu
Background Methotrexate (MTX) is widely used in clinical practice for the treatment of malignant tumors and autoimmune diseases. High-dose MTX has been shown to be an effective approach for treating various malignant tumors, but it is accompanied by numerous toxic side effects, necessitating therapeutic drug monitoring (TDM) for patients and timely “folinic acid rescue.” High-performance liquid chromatography and fluorescent immunoassay (FIA) are currently used to detect MTX, but these methods are limited by complex sample preparation, time consumption, and high cost. Therefore, a simple, rapid, and cost-effective MTX measurement method is required. Results We developed a flexible and inexpensive electrochemical sensor using a stearyl trimethyl ammonium bromide (STAB)-modified, screen-printed carbon electrode to directly detect MTX in human serum. Assay performance was validated via detection of MTX in spiked buffer. The sensor was capable of measuring MTX concentrations ranging from 0.01–1 μM and 1–1500 μM, with a limit of detection of 3.1 nM and a limit of quantitation of 3.5 nM. For the samples simulating combined medication, the sensor exhibited outstanding selectivity, in cross-reactivity, with the maximum response value of interferents reaching only 3.49%. Additionally, the sensor shows reliable repeatability with a relative standard deviation of 3.8% and remarkable stability. Recovery in human serum validated the clinical utility of the sensor in point-of-care testing conditions. The sensor’s applicability to personal medicine was confirmed by detecting MTX blood concentration in patients with different diseases. The results obtained by the sensor were compared with those obtained by the FIA technique, a method commonly used in hospitals, showing a high level of consistency between both methods. Significance To meet the requirements of personalized medicine for MTX-patients, we developed a disposable biosensor with wide detection range from 0.01–1 μM and 1–1500 μM. Owing to the effective enrichment of STAB, the electrochemical response was sensitive, selective, stable, and rapid. As per the clinical test results, our sensor has shown a high level of consistency with the FIA method, indicating its potential to replace FIA as a cost-effective platform for MTX-TDM.
研究领域:
药理学
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生物传感技术
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临床化学
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个性化医学
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 2
·272
·
IF 5.4
Dong Wang,Xiyu Mao,Yitao Liang,Yu Cai,Tingting Tu,Shanshan Zhang,Tianyu Li,Lu Fang,Yue Zhou,Zhaoyang Wang,Yu Jiang,Xuesong Ye,Bo Liang
Urine analysis is widely used in clinical practice to indicate human heathy status and is important for diagnosing chronic kidney disease (CKD). Ammonium ions (NH4+), urea, and creatinine metabolites are main clinical indicators in urine analysis of CKD patients. In this paper, NH4+selective electrodes were prepared using electropolymerized polyaniline-polystyrene sulfonate (PANI: PSS), and urea- and creatinine-sensing electrodes were prepared by modifying urease and creatinine deiminase, respectively. First, PANI: PSS was modified on the surface of an AuNPs-modified screen-printed electrode, as a NH4+-sensitive film. The experimental results showed that the detection range of the NH4+selective electrode was 0.5~40 mM, and the sensitivity reached 192.6 mA M−1cm−2with good selectivity, consistency, and stability. Based on the NH4+-sensitive film, urease and creatinine deaminase were modified by enzyme immobilization technology to achieve urea and creatinine detection, respectively. Finally, we further integrated NH4+, urea, and creatinine electrodes into a paper-based device and tested real human urine samples. In summary, this multi-parameter urine testing device offers the potential for point-of-care testing of urine and benefits the efficient chronic kidney disease management.Keywords:electrochemical biosensor;PANI: PSS;ammonium ion;urea;creatinine;urinalysis
研究领域:
分析化学
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生物医学工程
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临床诊断
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Hongyuan Zhang,Jieqiong Wang,Wei Wu,Ce Han,Ming Li
A novel electrochemical sensor for detecting heavy metal ions in seafood was developed to address food safety concerns. The sensor integrates graphene oxide into NH 2 -UiO-66 loaded nanofiber carbon aerogel, enhanced its three-dimensional conductive network and effective active surface area (0.34 cm 2 ), which improved ion enrichment and oxidation-reduction reaction rates. Using the Box-Behnken design, the sensor was optimized to detect cadmium and lead with LODs of 0.16 μg·L −1 and 0.07 μg·L −1 in the 1 to 150 μg·L −1 linear range. Applied to real seafood samples, the sensor showed results consistent with ICP-OES, confirmed its reliability in complex substrates. This approach offered a promising, low-cost solution for real-time monitoring of heavy metal contamination in seafood, ensuring public food safety.
研究领域:
食品科学
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电化学传感器
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材料科学
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产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
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2025
·
IF 10
Hongguang Zhang,Zi Yu,Yongxin Liang,Tao Gan,Zuqiang Huang,Huayu Hu,Yanjuan Zhang
The utilization of organic molecules as in-situ hydrogen donors for producing γ-valerolactone (GVL) biofuel from biomass-derived levulinic acid (LA) represents one of the promising sustainable approaches. However, this process still faces challenges, including the difficult activation of the hydrogen source and the transport limitations of active hydrogen species. Herein, a Co-MnO/spontaneously polarized ceramic-carbon (Co-MnO/SPCE-C) composite catalyst, featuring a unique spontaneous polarization effect, was developed for catalytic transfer hydrogenation (CTH) of LA to GVL in the isopropanol/H 2 O (volume ratio = 1/19) cosolvent system. Co-MnO/SPCE-C contributed to an outstanding LA conversion of 99.9% and a favorable GVL yield of 93.5%, surpassing the performance achieved by comparative catalysts. Various characterization techniques demonstrate that SPCE with far-infrared emission and surface electric field increased the oxygen vacancies, thereby promoting hydrogen spillover and enhancing the properties of CoO x –MnO acidic sites. The 17 O NMR spectra reveal that SPCE activated water clusters by weakening the hydrogen bonding network. Moreover, D 2 O experiments show that SPCE facilitated the participation of water in the reaction. This work provides valuable insights into the development of an efficient strategy for producing GVL from LA. Before the catalyst can be considered for commercial applications, the focus in the future should be on a comprehensive computational analysis, long-term evaluation of catalyst stability, and additional industrial-scale testing.
研究领域:
化学工程
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材料科学
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催化反应
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2022
·
IF 12.545
Jinrong Liu,Chong Ma,Siwei Shi,Heng Liu,Wei Wen,Xiuhua Zhang,Zhen Wu,Shengfu Wang
As an important component of the COVID-19 mRNA vaccines, liposomes play a key role in the efficient protection and delivery of mRNA to cells. Herein, due to the controllable release amplification strategy of liposomes, a reliable and robust single-particle collision electrochemical (SPCE) biosensor was constructed for H9N2 avian influenza virus (H9N2 AIV) detection by combining liposome encapsulation-release strategy with immunomagnetic separation. The liposomes modified with biotin and loaded with platinum nanoparticles (Pt NPs) were used as signal probes for the first time. Biotin facilitated the coupling of biomolecules (DNA or antibodies) through the specific reaction of biotin-streptavidin. Each liposome can encapsulate multiple Pt NPs, which were ruptured under the presence of 1 × PBST (phosphate buffer saline with 0.05% Tween-20) within 2 min, and the encapsulated Pt NPs were released for SPCE experiment. The combination of immunomagnetic separation not only improved the anti-interference capabilities but also avoided the agglomeration of Pt NPs, enabling the SPCE biosensor to realize ultrasensitive detection of 18.1 fg/mL H9N2 AIV. Furthermore, the reliable SPCE biosensor was successfully applied in specific detection of H9N2 AIV in complex samples (chicken serum, chicken liver and chicken lung), which promoted the universality of SPCE biosensor and its application prospect in early diagnosis of diseases.
研究领域:
生物化学
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分析化学
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病毒学
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生物医学工程
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2014
·
IF 6.451
Shuo-Hui Cao,Zhe-Xiang Zou,Yu-Hua Weng,Wei-Peng Cai,Qian Liu,Yao-Qun Li
In this article, plasmon-mediated fluorescence biosensing is reported to be distance independent through a full-coupling strategy that effectively activates the entire plasmon coupling region. This concept is demonstrated through collecting the directional surface plasmon-coupled emission (SPCE) signal from fluorescent silica nanoparticles with a size that matches the entire coupling region. Based on this design, the spatial distribution of the fluorophores is confined by the dimension of the nanoparticle. Therefore, these encapsulated fluorophores occupy the maximum coupling dominant region and optimally utilize the coupling effect. Being different from the conventional plasmon-mediated fluorescence, the enhanced fluorescence response becomes nearly independent of distance changes on a wide dynamic range from 0 nm to 30 nm between the fluorescent nanoparticles and metal structure. Full-coupling SPCE appropriately enlarges the distribution of fluorophores, ensuring that the coupling dominant region is filled with enough fluorophores at varying distances to create a stable and detectable signal. This scale of distances is well suited for many biorecognition events. Full-coupling SPCE solves signal deviation challenges originating from the susceptible and unpredictable orientation and conformation of biomolecules on the nanoscale . Immunoassays and DNA detection are shown with high and reliable signals, demonstrating the advantages of distance-independent full coupling. Without the need of a complicated and rigorous architecture for precise distance control, full-coupling SPCE offers great promise for a general platform of chip-based biosensing and bioanalysis.
研究领域:
光学
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生物传感
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纳米技术
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分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Yujiao Hou,Nan Long,Qingbin Xu,Ying Li,Pengyue Song,Minghan Yang,Jiabo Wang,Lidong Zhou,Ping Sheng,Weijun Kong
Trace detection of ochratoxin A (OTA) in foods is essential to mitigate risks to human health. Herein, a label-free electrochemical (EC) aptasensor based on dual-signal amplification of Nafion dispersed multi-walled carbon nanotubes (Nafion-MWCNTs) and Au nanopopcorns was developed for ultrasensitive detection of OTA. Nafion solution prevented the leaching of MWCNTs, and the Nafion-MWCNTs modified screen-printed carbon electrode (SPCE) acted as the sensing substrate which facilitated the uniform distribution of the electrodeposited Au nanopopcorns. The in-situ generated Au nanopopcorns could not only load a large amount of aptamers for specific identification of OTA, but also promote the electron transfer of the sensing platform. The incorporation of Nafion-MWCNTs and Au nanopopcorns realized dual-amplification of the aptasensor due to the enhanced conductivity and the increased electroactive surface area of the electrode. The modified electrodes were characterized through scanning electron microscopy, X-ray photoelectron spectroscopy and EC evaluation. Under optimal conditions, the electrochemical impedance spectroscopy (EIS) was measured for the determination of OTA. The as-fabricated Au nanopopcorns/Nafion-MWCNTs impedimetric aptasensor displayed excellent sensitivity with a detection limit as low as 1 pg/mL and a wide linear range of 1 pg/mL-10 ng/mL for OTA. Practical application of the aptasensor in the spiked malt samples achieved satisfactory recoveries of 89.82–95.65 %, which was also successfully verified to detect OTA in eleven batches of actual malt samples collected from the local market. The creative aptasensor is simple, cost-effective, sensitive, and accurate, showing great promise for on-site monitoring of other trace contaminants in foods by simply replacing the aptamers.
研究领域:
分析化学
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食品科学
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纳米技术
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毒理学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-8
·
IF 5.3
Han Lei,Cai Shuanglong,Chen Xiaogeng
The development of an ultrasensitive and precise measurement of a breast cancer biomarker (cancer antigen 15-3; CA15-3) in complex human serum is essential for the early diagnosis of cancer in groups of healthy populations and the treatment of patients. However, currently available testing technologies suffer from insufficient sensitivity toward CA15-3, which severely limits early large-scale screening of breast cancer patients. We report a versatile electrochemical immunoassay method based on atomically cobalt-dispersed nitrogen-doped carbon (Co-NC)–modified disposable screen-printed carbon electrode (SPCE) with alkaline phosphatase (ALP) and its metabolite, ascorbic acid 2-phosphate (AAP), as the electrochemical labeling and redox signaling unit for sensitive detection of low-abundance CA15-3. During electrochemical detection by differential pulse voltammetry (DPV), it was found that the Co-NC-SPCE electrode did not have a current signal response to the AAP substrate; however, it had an extremely favorable response current to ascorbic acid (AA). Based on the above principle, the target CA15-3-triggered immunoassay enriched ALP-catalyzed AAP produces a large amount of AA, resulting in a significant change in the system current signal, thereby realizing the highly sensitive detection of CA15-3. Under the optimal AAP substrate concentration and ALP catalysis time, the Co-NC-SPCE-based electrochemical immunoassay demonstrated a good DPV current for CA15-3 in the assay interval of 1.0 mU/mL to 10,000 mU/mL, with a calculated limit of detection of 0.38 mU/mL. Since Co-NC-SPCE has an excellent DPV current response to AA and employs split-type scheme, the constructed electrochemical immunoassay has the merits of high preciseness and anti-interference, and its clinical diagnostic results are comparable to those of commercial kits. Graphical abstract
研究领域:
分析化学
·
生物化学
·
电化学
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）