Nanomaterials
·
2022
·Vol. 12
·期 13
·2219
·
IF 5.719
Haoyuan Cai,Mengwei Wang,Zhuohui Wu,Jing Liu,Xiaoping Wang
We investigate a high-sensitivity surface plasmon resonance (SPR) biosensor consisting of a Au layer, four-layer MoS2, and monolayer graphene. The numerical simulations, by the transfer matrix method (TMM), demonstrate the sensor has a maximum sensitivity of 282°/RIU, which is approximately 2 times greater than the conventional Au-based SPR sensor. The finite difference time domain (FDTD) indicates that the presence of MoS2film generates a strong surface electric field and enhances the sensitivity of the proposed SPR sensor. In addition, the influence of the number of MoS2layers on the sensitivity of the proposed sensor is investigated by simulations and experiments. In the experiment, MoS2and graphene films are transferred on the Au-based substrate by the PMMA-based wet transfer method, and the fabricated samples are characterized by Raman spectroscopy. Furthermore, the fabricated sensors with the Kretschmann configuration are used to detect okadaic acid (OA). The okadaic acid–bovine serum albumin bioconjugate (OA-BSA) is immobilized on the graphene layer of the sensors to develop a competitive inhibition immunoassay. The results show that the sensor has a very low limit of detection (LOD) of 1.18 ng/mL for OA, which is about 22.6 times lower than that of a conventional Au biosensor. We believe that such a high-sensitivity SPR biosensor has potential applications for clinical diagnosis and immunoassays.Keywords:biosensor;MoS2;graphene;SPR sensor;high sensitivity
研究领域:
生物医学工程
·
材料科学
·
光学传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Chao Zhang,Zhe Li,Shou Zhen Jiang,Chong Hui Li,Shi Cai Xu,Jing Yu,Zhen Li,Ming Hong Wang,Ai Hua Liu,Bao Yuan Man
We present a U-bent fiber optic SPR sensor based on the graphene/AgNPs, combined the virtues of graphene, AgNPs and U-bent fiber. By changing the laser-induced time, we achieved the optimal time for the deposition of AgNPs. With the proposed graphene/AgNPs U-bent fiber optic sensor, we discussed and investigated the SPR behaviors. The shifts up to 32 and 16 nm are observed respectively for the detection of 90% aqueous ethanol and 20% aqueous glucose, corresponding refractive index (RI) 1.3657 and 1.3557. The sensitivity of this proposed is calculated as 1198 nm/RIU and the response and recovery time of this U-bent fiber optic sensor are respectively 3 s and 80 s. These experimental results indicate that our proposed graphene/AgNPs U-bent fiber optic sensor will be expected to open new avenue for the detection in the field of medicine, biotechnology and food safety.
研究领域:
生物医学工程
·
光学传感
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Youquan Zhou,Xiaoyu Huang,Binyun Xia,Xiaohan Chen,Xinyue Jing,Ning Wang,Jinhua Li
Precise detection of ferric ions (Fe 3+ ) plays a critical role in maintaining biological homeostasis, ensuring industrial process quality control, and safeguarding ecological safety. Recently, various detection methods and sensors have been widely developed for measuring Fe 3+ , but the tradeoff between their performance and practical applications has not been achieved, such as high sensitivity, excellent specificity, simple structure and fabrication, low cost, and so on. In this study, an optical fiber SPR sensor was developed sensor for detecting Fe 3+ based on the multimode-coreless-multimode (MNM) structured optical fiber. The refractive index (RI) sensitivity of the MNM fiber coated with a 60 nm Au film modified with gold nanoparticles (AuNPs) reached 3440.45 nm/RIU. The organic groups functionalized carbon quantum dots (CQDs) were synthesized and immobilized onto the Au substrates for specifically trapping Fe 3+ , which will cause the variation of the effective RI around the fiber, thereby resulting in the SPR wavelength shift in the transmission spectrum. By recording the red shift of the SPR wavelength, the amount of Fe 3+ could be accurately quantified. Upon detection of Fe 3+ , the sensor was demonstrated with great linearity within a wide detection range of 10 −12 to 10 −2 M. The sensor’s sensitivity was obtained to be about 2.07 nm/lg (M) and its limit of detection (LOD) was calculated to be about 2.88 × 10 −13 M. It was also experimentally proved with excellent selectivity and stability, as well as the potential application in simulated real samples. The proposed sensing platform offered a simple configuration for Fe 3+ detection with excellent performance and had various potential practical applications such as health monitoring, disease diagnosis, dietary planning, medication treatment monitoring, environmental monitoring, etc.
研究领域:
生物医学工程
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Zhiyong Yin,Zhibing Zhang,Xili Jing,Linchuan Hu,Shuguang Li,Jianshe Li
Background Surface plasmon resonance (SPR) sensing technology has been widely used in biometrics, but the weak detection capability and low sensitivity limit the development of SPR biosensors. In this work, we propose to employ the transition metal disulfide (TMD) material MoS 2 to induce the SPR effect into the near-infrared band. The aim of this work is to develop a near-infrared sensor capable of quantitatively detecting the concentration of cDNA, which is able to solve the problems of low sensitivity, parameter crosstalk and so on. Results The results show that the sensitivity of the SPR sensor at infrared wavelengths is 1.69 times higher than that of visible, and the infrared wave is more suitable as an excitation source for the SPR effect because it has a stronger evanescent field. In addition, we have prepared a DNA hybridization sensor that can work in the near-infrared band to detect complementary DNA (cDNA) concentration. Moreover, a combined cascade and parallel strategy realized the temperature and PH detection. The sensor uses the photonic crystal fiber as the platform, silver film as an excitation layer, and MoS 2 as a modulation layer. The optimal parameters of the sensor were determined during the experiment, and the three-parameter detection was successfully realized. The experimental results show that the three sensing channels can work independently, and the DNA hybridization probe can achieve selective detection of cDNA with a sensitivity as high as 0.22 nm/(nmol/L). Significance The proposed three-channel DNA hybridization sensor has high sensitivity and accuracy. The innovation of this work is to demonstrate that infrared waves are more suitable to be used as the excitation source of SPR sensors and that the proposed three-channel sensor has a promising future in early diagnosis and biosensing.
研究领域:
生物传感
·
分子生物学
·
光学工程
✓ 已验证
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Rui Wang,Chunlan Liu,Yong Wei,Ze Ran,Tianci Jiang,Chunbiao Liu,Chen Shi,Zhuo Ren,Xingkai Wang,Zhihai Liu,Yu Zhang
The concentration of tumor markers is low, which needs a highly sensitive, stable and fast detection method. In this paper, we proposed and demonstrated a U-shape fiber SPR biosensor sensitized by MOFs materials. The surface of the U-shape SPR sensor was modified with MOFs materials to enhance the sensitivity, and the nucleic acid aptamer was immobilized on the sensor surface because of the biocompatibility of MOFs materials. By the high specificity of the nucleic acid aptamer, the MUC1 protein was recognized and detected. The testing results indicate that the sensor has a logarithmic linear response in the MUC1 protein concentration detection range of 1 pg/ml-100 μg/ml, its sensitivity and detection limit are 5.33 nm/log(μg/ml) and 0.16 pg/ml respectively. After being sensitized by MOFs, the detection sensitivity of the sensor can be increased by 1.62 times，the LOD can be decreased by 0.75 times. The sensor has high sensitivity and specificity, which has broad application prospects in clinical detection of tumor markers.
研究领域:
生物医学工程
·
肿瘤标志物检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IEEE SENSORS JOURNAL
·
2024
·
IF 4.3
Lingling Li,Shen Yu,Chonglu Jing,Lihua Chen,Ai Zhou
In order to deal with the issue of low sensitivity to biomolecules concentrations in traditional optical fiber surface plasmon resonance (SPR) biosensors, a fiber SPR deoxyribonucleic acid (DNA) biosensor was developed, which is based on an amino-functionalized Zr-based metal–organic framework (MOF) (UIO-66-NH2)-modified heterogeneous core structure. Porous material UIO-66-NH2 facilitates electron transfer and amplifies the SPR effect, resulting in a heightened sensitivity for the detection of DNA hybridization. This can be attributed to the material’s advantageous characteristics, including a substantial specific surface area, an excellent water stability, and a pronounced affinity for probe-DNA (pDNA). The DNA biosensor presented in this study demonstrates enhanced sensitivity as a result of the covalent attachment of MOFs to pDNA, hence increasing the number of binding sites available for target DNA (tDNA). The optical fiber SPR biosensor, which has been modified with UIO-66-NH2, exhibits remarkable capabilities in detecting DNA concentrations ranging from 1 pM to 1 μM . It demonstrates a sensitivity of −7.72 nm/log ( μM ) and achieves a detection limit of 3 pM. In addition, the DNA biosensor has exceptional performance in terms of specificity and durability, rendering it a highly favorable option for utilization in several fields, such as drug delivery, protein identification, and environmental surveillance.
研究领域:
生物传感技术
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
IEEE SENSORS JOURNAL
·
2024
·Vol. 24
·期 6
·7932-7938
·
IF 4.3
Chunlan Liu,Ziqiang Zhang,Yabin Shao,Yong Wei,Puxi Ren,Yixiong Tang,Mingjun Cai,Zhihai Liu
Traditional methods for chromium ion detection have problems, such as slow detection speed, long detection cycle, and difficulty in on-site detection. Although fiber surface plasmon resonance (SPR) sensor has overcome these problems, their sensitivity cannot meet the detection requirements for chromium ion detection. Based on this, this article proposes a fiber SPR sensor based on a double helix loopback structure. By adjusting the parameters of the double helix structure, the light incidence angle in the sensing area was adjusted to improve the sensitivity. The symmetrical double helix structure increases the surface area of the sensing area and also enhances the structural strength of the sensor, and its optimal sensitivity is 6356 nm/RIU. After functionalizing the surface of the sensing probe, it is used for specific detection of chromium ion concentration, with a detection limit as low as 0.15 ng/mL, which achieves the rapid, real-time, and highly sensitive specific detection of chromium ions.
研究领域:
传感技术
·
环境监测
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS
·
2025
·Vol. 25
·期 5
·1630
·
IF 3.5
Qiang Xu,Huiting Yin,Mei Cui,Renliang Huang,Rongxin Su
A fiber-reinforced SPR sensor based on silver-nucleated gold-shell bimetallic nanoparticles and graphene oxide was developed and applied to human IgG detection. The refractive index (RI) sensitivity of the Ag@Au/GO fiber SPR sensor is as high as 4715.9 nm/RIU in the RI range of 1.333–1.365. Staphylococcus aureus protein A (SPA) can specifically recognize and bind to the fragment crystallizable (Fc) of the antibody; it facilitates the highly targeted immobilization of the antibody. SPA and rabbit anti-human IgG were immobilized on the surface of the Ag@Au/GO fiber SPR sensor for the detection of different concentrations of human IgG with a sensitivity of 0.53 nm/μg/mL and detection limits of 0.037 μg/mL. This biosensor based on the mixed structure of GO and Ag@Au combined the common advantages of the two materials. Therefore, our study provides a simple platform for biological analysis and has a good application prospect.
研究领域:
生物传感技术
·
材料科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OPTICS EXPRESS
·
2023
·Vol. 31
·期 23
·38179-38190
·
IF 3.8
Yong Wei,Chen Shi,Yonghui Zhang,Chunbiao Liu,Yixiong Tang,Puxi Ren,Chen Wang,Yu Zhang,Zhihai Liu
The current temperature-compensated fiber-optic surface plasmon resonance (SPR) biosensors are mainly open-ended outside the sensing structure, and there is a lack of temperature compensation schemes in fiber-optic microfluidic chips. In this paper, we proposed a temperature-compensated optical fiber SPR microfluidic sensor based on micro-nano 3D printing. Through the optical fiber micro-machining technology, the two sensing areas were designed on both sides of the same sensing fiber. The wavelength division multiplexing technology was used to collect the sensing light signals of the two sensing areas at the same time. The specific measurement of berberine and the detection of ambient temperature in the optical fiber SPR biological microfluidic channel were realized, and the temperature compensation matrix relationship was constructed, and then the temperature compensation was realized when measuring berberine biomolecules. Experiments have shown that the temperature sensitivity of the optical fiber SPR microfluidic sensor was 2.18 nm/°C, the sensitivity of the detection of berberine was 0.2646 nm/(µg/ml), the detection limit (LOD) was 0.38 µg/ml, and in a mixed solution showed an excellent specific detection impact.
研究领域:
生物医学工程
·
光学传感
·
微流控技术
✓ 已验证
DOI
高影响因子
Nanophotonics
·
2019
·Vol. 9
·期 2
·327-336
·
IF 6.908
Yuting Zhao,Shuaiwen Gan,Leiming Wu,Jiaqi Zhu,Yuanjiang Xiang,Xiaoyu Dai
Germanium selenide (GeSe) nanosheets are stable and inexpensive and considered to have a great potential for photovoltaic applications, however we have demonstrated that GeSe nanosheets are also promising for sensing technology, in this paper. By spin-coating the GeSe nanosheets on the surface of noble metal (Au), we have obtained a surface plasmon resonance (SPR) sensor with significantly enhanced sensitivity, and the performance of the sensor is closely related to the thickness of the GeSe film. By detecting different refractive index solutions, we have obtained the optimized sensitivity with 3581.2 nm/RIU (which is nearly 80% improvement compared to traditional SPR sensors) and figure of merit with 14.37 RIU −1 . Moreover, the proposed SPR sensor was vastly superior in sensing Pb 2+ heavy metal ions after coating it with chitosan and GeSe composite. A maximum sensitivity of 30.38 nm/μg/l has been verified, which is nearly six times better than that of conventional SPR sensor. Our results demonstrated that GeSe nanosheets overlayer with modified SPR sensor has its great potential in heavy metal detection and chemical-specific molecular identification.
研究领域:
纳米技术
·
光学
·
传感器
·
环境科学
✓ 已验证
DOI
高影响因子
OPTICS AND LASERS IN ENGINEERING
·
2020
·
IF 4.273
Hang Song,Qi Wang,Wan-Ming Zhao
In this work, we proposed a novel surface plasmon resonance (SPR) sensitivity-enhancing method by adding MoS 2 nanosheets between gold film and fiber (fiber-MoS 2 -gold film). And we apply it to the detection of human IgG protein. The sensor was fabricated by attaching MoS 2 nanosheets to the U-shaped fiber surface and then covering a layer of gold film. The refractive index sensitivity of 6184.4 nm/RIU was achieved by the fiber-MoS 2 -gold film sensor which is 59 percent higher than the gold film SPR sensor and 25 percent than the fiber-gold film-MoS 2 SPR sensor.The presence of MoS 2 increases the absorption of light energy and excites a stronger SPR phenomenon. At the same time, since the monolayer MoS 2 is a direct bandgap semiconductor, the absorbed light energy promotes the transfer of electrons, generates strong coupling on the gold film surface, enhances the surface electric field, and improves the sensitivity of the sensor. Biofunctionalized sensor detect human IgG with the limit of detection as low as 19.7 ng/mL. This work proposed a novel method for enhancing the sensitivity of SPR sensor, it owns a higher sensitivity and a lower LOD which has broad application prospects in measuring low concentration biological analytes.
研究领域:
光学
·
生物传感
·
纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
OPTICS AND LASER TECHNOLOGY
·
2024
·
IF 4.6
Yanpei Xu,Yutong Song,Haixing Hao,Zhimeng Zhao,Yong Jin,Qi Wang
ZIF-8 is a typical metal–organic framework (MOF) material, which has great potential for sensor improvement. In this paper, we prepared a high sensitivity surface plasmon resonance (SPR) sensor based on ZIF-8/Au and described the preparation process of the sensor and characterized the samples in detail. The results showed that the synthesized ZIF-8 nanoparticles were uniform with an average diameter of about 50 nm. The performance of the sensor was related to the amount of ZIF-8, which could be controlled by adjusting the number of spin-coating cycles. When the refractive index ranged from 1.3335 to 1.3635, the maximum sensitivity of the sensor reached 3961.36nm/RIU with one cycle of spin-coating, which was 74.05% higher than that of pure Au single-layer film sensor. Furthermore, the feasibility of the proposed sensor for biological sensing was demonstrated by detecting bovine serum albumin (BSA).
研究领域:
材料科学
·
传感技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）