Nanomaterials
·
2022
·Vol. 12
·期 13
·2219
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IF 5.719
Haoyuan Cai,Mengwei Wang,Zhuohui Wu,Jing Liu,Xiaoping Wang
We investigate a high-sensitivity surface plasmon resonance (SPR) biosensor consisting of a Au layer, four-layer MoS2, and monolayer graphene. The numerical simulations, by the transfer matrix method (TMM), demonstrate the sensor has a maximum sensitivity of 282°/RIU, which is approximately 2 times greater than the conventional Au-based SPR sensor. The finite difference time domain (FDTD) indicates that the presence of MoS2film generates a strong surface electric field and enhances the sensitivity of the proposed SPR sensor. In addition, the influence of the number of MoS2layers on the sensitivity of the proposed sensor is investigated by simulations and experiments. In the experiment, MoS2and graphene films are transferred on the Au-based substrate by the PMMA-based wet transfer method, and the fabricated samples are characterized by Raman spectroscopy. Furthermore, the fabricated sensors with the Kretschmann configuration are used to detect okadaic acid (OA). The okadaic acid–bovine serum albumin bioconjugate (OA-BSA) is immobilized on the graphene layer of the sensors to develop a competitive inhibition immunoassay. The results show that the sensor has a very low limit of detection (LOD) of 1.18 ng/mL for OA, which is about 22.6 times lower than that of a conventional Au biosensor. We believe that such a high-sensitivity SPR biosensor has potential applications for clinical diagnosis and immunoassays.Keywords:biosensor;MoS2;graphene;SPR sensor;high sensitivity
研究领域:
生物医学工程
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材料科学
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光学传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Chao Zhang,Zhe Li,Shou Zhen Jiang,Chong Hui Li,Shi Cai Xu,Jing Yu,Zhen Li,Ming Hong Wang,Ai Hua Liu,Bao Yuan Man
We present a U-bent fiber optic SPR sensor based on the graphene/AgNPs, combined the virtues of graphene, AgNPs and U-bent fiber. By changing the laser-induced time, we achieved the optimal time for the deposition of AgNPs. With the proposed graphene/AgNPs U-bent fiber optic sensor, we discussed and investigated the SPR behaviors. The shifts up to 32 and 16 nm are observed respectively for the detection of 90% aqueous ethanol and 20% aqueous glucose, corresponding refractive index (RI) 1.3657 and 1.3557. The sensitivity of this proposed is calculated as 1198 nm/RIU and the response and recovery time of this U-bent fiber optic sensor are respectively 3 s and 80 s. These experimental results indicate that our proposed graphene/AgNPs U-bent fiber optic sensor will be expected to open new avenue for the detection in the field of medicine, biotechnology and food safety.
研究领域:
生物医学工程
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光学传感
·
材料科学
✓ 已验证
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Rui Wang,Chunlan Liu,Yong Wei,Ze Ran,Tianci Jiang,Chunbiao Liu,Chen Shi,Zhuo Ren,Xingkai Wang,Zhihai Liu,Yu Zhang
The concentration of tumor markers is low, which needs a highly sensitive, stable and fast detection method. In this paper, we proposed and demonstrated a U-shape fiber SPR biosensor sensitized by MOFs materials. The surface of the U-shape SPR sensor was modified with MOFs materials to enhance the sensitivity, and the nucleic acid aptamer was immobilized on the sensor surface because of the biocompatibility of MOFs materials. By the high specificity of the nucleic acid aptamer, the MUC1 protein was recognized and detected. The testing results indicate that the sensor has a logarithmic linear response in the MUC1 protein concentration detection range of 1 pg/ml-100 μg/ml, its sensitivity and detection limit are 5.33 nm/log(μg/ml) and 0.16 pg/ml respectively. After being sensitized by MOFs, the detection sensitivity of the sensor can be increased by 1.62 times，the LOD can be decreased by 0.75 times. The sensor has high sensitivity and specificity, which has broad application prospects in clinical detection of tumor markers.
研究领域:
生物医学工程
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肿瘤标志物检测
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产品关联
DOI
PubMed
高影响因子
IEEE SENSORS JOURNAL
·
2024
·
IF 4.3
Lingling Li,Shen Yu,Chonglu Jing,Lihua Chen,Ai Zhou
In order to deal with the issue of low sensitivity to biomolecules concentrations in traditional optical fiber surface plasmon resonance (SPR) biosensors, a fiber SPR deoxyribonucleic acid (DNA) biosensor was developed, which is based on an amino-functionalized Zr-based metal–organic framework (MOF) (UIO-66-NH2)-modified heterogeneous core structure. Porous material UIO-66-NH2 facilitates electron transfer and amplifies the SPR effect, resulting in a heightened sensitivity for the detection of DNA hybridization. This can be attributed to the material’s advantageous characteristics, including a substantial specific surface area, an excellent water stability, and a pronounced affinity for probe-DNA (pDNA). The DNA biosensor presented in this study demonstrates enhanced sensitivity as a result of the covalent attachment of MOFs to pDNA, hence increasing the number of binding sites available for target DNA (tDNA). The optical fiber SPR biosensor, which has been modified with UIO-66-NH2, exhibits remarkable capabilities in detecting DNA concentrations ranging from 1 pM to 1 μM . It demonstrates a sensitivity of −7.72 nm/log ( μM ) and achieves a detection limit of 3 pM. In addition, the DNA biosensor has exceptional performance in terms of specificity and durability, rendering it a highly favorable option for utilization in several fields, such as drug delivery, protein identification, and environmental surveillance.
研究领域:
生物传感技术
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
IEEE SENSORS JOURNAL
·
2024
·Vol. 24
·期 6
·7932-7938
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IF 4.3
Chunlan Liu,Ziqiang Zhang,Yabin Shao,Yong Wei,Puxi Ren,Yixiong Tang,Mingjun Cai,Zhihai Liu
Traditional methods for chromium ion detection have problems, such as slow detection speed, long detection cycle, and difficulty in on-site detection. Although fiber surface plasmon resonance (SPR) sensor has overcome these problems, their sensitivity cannot meet the detection requirements for chromium ion detection. Based on this, this article proposes a fiber SPR sensor based on a double helix loopback structure. By adjusting the parameters of the double helix structure, the light incidence angle in the sensing area was adjusted to improve the sensitivity. The symmetrical double helix structure increases the surface area of the sensing area and also enhances the structural strength of the sensor, and its optimal sensitivity is 6356 nm/RIU. After functionalizing the surface of the sensing probe, it is used for specific detection of chromium ion concentration, with a detection limit as low as 0.15 ng/mL, which achieves the rapid, real-time, and highly sensitive specific detection of chromium ions.
研究领域:
传感技术
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环境监测
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Zhiyong Yin,Zhibing Zhang,Xili Jing,Linchuan Hu,Shuguang Li,Jianshe Li
Background Surface plasmon resonance (SPR) sensing technology has been widely used in biometrics, but the weak detection capability and low sensitivity limit the development of SPR biosensors. In this work, we propose to employ the transition metal disulfide (TMD) material MoS 2 to induce the SPR effect into the near-infrared band. The aim of this work is to develop a near-infrared sensor capable of quantitatively detecting the concentration of cDNA, which is able to solve the problems of low sensitivity, parameter crosstalk and so on. Results The results show that the sensitivity of the SPR sensor at infrared wavelengths is 1.69 times higher than that of visible, and the infrared wave is more suitable as an excitation source for the SPR effect because it has a stronger evanescent field. In addition, we have prepared a DNA hybridization sensor that can work in the near-infrared band to detect complementary DNA (cDNA) concentration. Moreover, a combined cascade and parallel strategy realized the temperature and PH detection. The sensor uses the photonic crystal fiber as the platform, silver film as an excitation layer, and MoS 2 as a modulation layer. The optimal parameters of the sensor were determined during the experiment, and the three-parameter detection was successfully realized. The experimental results show that the three sensing channels can work independently, and the DNA hybridization probe can achieve selective detection of cDNA with a sensitivity as high as 0.22 nm/(nmol/L). Significance The proposed three-channel DNA hybridization sensor has high sensitivity and accuracy. The innovation of this work is to demonstrate that infrared waves are more suitable to be used as the excitation source of SPR sensors and that the proposed three-channel sensor has a promising future in early diagnosis and biosensing.
研究领域:
生物传感
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分子生物学
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光学工程
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Zhuoyue Zhou,Zhao Yang,Li Xia,Houjin Zhang
Chiral analysis of amino acids (AAs) is of great importance in medical science due to the distinctive effect of AA isomers on human health. Although various chiral recognition techniques have been developed, the quantitative chiral recognition of low-level AA isomers remains challenging. Here, we combined the fiber optic SPR with an enzyme-substrate recognition mechanism to construct a direct-assay-type chiral AA biosensor. As a proof-of-concept attempt, a recently discovered Rasamsonia emersonii D-amino acid oxidase (ReDAAO) with a wide substrate spectrum and high stability was immobilized on the graphene oxide and gold nanorods composites (GO-AuNRs), using both EDC/NHS coupling and the gold-binding peptide (GBP) method. Such a biosensor can distinguish two AA isomers at the same concentration. It achieved specific detection of D-amino acids (D-AAs) with a linear range from 5x10 -4 mM to 30 mM. Furthermore, it showed good resistance to enantiomeric interference. When the percentage of D-AA increases in the isomer mixture, a good linear relationship between the D/(D + L)-AA ratio and SPR spectral shift was obtained. This unique combination of the enzyme , nanocomposite , and SPR taps into the rich reservoir of proteins for chiral receptors. It lays the foundation for protein-based chiral recognition of other clinically important small molecules in future biosensor designs.
研究领域:
生物传感器
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分析化学
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生物医学工程
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纳米技术
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酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Youquan Zhou,Xiaoyu Huang,Binyun Xia,Xiaohan Chen,Xinyue Jing,Ning Wang,Jinhua Li
Precise detection of ferric ions (Fe 3+ ) plays a critical role in maintaining biological homeostasis, ensuring industrial process quality control, and safeguarding ecological safety. Recently, various detection methods and sensors have been widely developed for measuring Fe 3+ , but the tradeoff between their performance and practical applications has not been achieved, such as high sensitivity, excellent specificity, simple structure and fabrication, low cost, and so on. In this study, an optical fiber SPR sensor was developed sensor for detecting Fe 3+ based on the multimode-coreless-multimode (MNM) structured optical fiber. The refractive index (RI) sensitivity of the MNM fiber coated with a 60 nm Au film modified with gold nanoparticles (AuNPs) reached 3440.45 nm/RIU. The organic groups functionalized carbon quantum dots (CQDs) were synthesized and immobilized onto the Au substrates for specifically trapping Fe 3+ , which will cause the variation of the effective RI around the fiber, thereby resulting in the SPR wavelength shift in the transmission spectrum. By recording the red shift of the SPR wavelength, the amount of Fe 3+ could be accurately quantified. Upon detection of Fe 3+ , the sensor was demonstrated with great linearity within a wide detection range of 10 −12 to 10 −2 M. The sensor’s sensitivity was obtained to be about 2.07 nm/lg (M) and its limit of detection (LOD) was calculated to be about 2.88 × 10 −13 M. It was also experimentally proved with excellent selectivity and stability, as well as the potential application in simulated real samples. The proposed sensing platform offered a simple configuration for Fe 3+ detection with excellent performance and had various potential practical applications such as health monitoring, disease diagnosis, dietary planning, medication treatment monitoring, environmental monitoring, etc.
研究领域:
生物医学工程
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分析化学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
OPTICS EXPRESS
·
2023
·Vol. 31
·期 23
·38179-38190
·
IF 3.8
Yong Wei,Chen Shi,Yonghui Zhang,Chunbiao Liu,Yixiong Tang,Puxi Ren,Chen Wang,Yu Zhang,Zhihai Liu
The current temperature-compensated fiber-optic surface plasmon resonance (SPR) biosensors are mainly open-ended outside the sensing structure, and there is a lack of temperature compensation schemes in fiber-optic microfluidic chips. In this paper, we proposed a temperature-compensated optical fiber SPR microfluidic sensor based on micro-nano 3D printing. Through the optical fiber micro-machining technology, the two sensing areas were designed on both sides of the same sensing fiber. The wavelength division multiplexing technology was used to collect the sensing light signals of the two sensing areas at the same time. The specific measurement of berberine and the detection of ambient temperature in the optical fiber SPR biological microfluidic channel were realized, and the temperature compensation matrix relationship was constructed, and then the temperature compensation was realized when measuring berberine biomolecules. Experiments have shown that the temperature sensitivity of the optical fiber SPR microfluidic sensor was 2.18 nm/°C, the sensitivity of the detection of berberine was 0.2646 nm/(µg/ml), the detection limit (LOD) was 0.38 µg/ml, and in a mixed solution showed an excellent specific detection impact.
研究领域:
生物医学工程
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光学传感
·
微流控技术
✓ 已验证
DOI
高影响因子
Nanoscale
·
2024
·
IF 5.8
Jiayi Zhu,Chao Zhao,Binyun Xia,Ning Wang,Xi Chen,Xinyue Jing,Minxuan Chen,Xinrui Xu
Abnormal human immunoglobulin G (IgG) may induce the risk of immune system disorder, infectious diseases, tumors and so on. However, the current detection methods exhibit low sensitivity, which limits their practical application. In this work, an SPR optical fiber sensor (SPR-OFS) with high sensitivity is designed for label-free detection of human IgG. It is fabricated using a heterostructure optical fiber coated with Au film/AuNPs and the Ti3C2Tx MXene biofunctionalized with goat anti-human IgG by polydopamine (PDA). In the experiment, the optimal thickness of the Ti3C2Tx MXene was explored and determined to be about 93 nm by comprehensively considering the refractive index (RI) sensitivity and spectral bandwidth of the SPR sensor. When the largest figure of merit (FOM) is calculated to be 17.8279 RIU−1, its RI sensitivity was ultimately found to be 2804.5 nm per RIU. The SPR-OFS was employed to detect human IgG within the concentration range of 0–30 μg mL−1 and its sensitivity is demonstrated to be 1.7046 nm (μg mL−1)−1. The SPR-OFS was also proved to have excellent linearity, specificity and stability. The proposed sensor offers outstanding performance with simple fabrication, providing a cutting-edge bioanalytical platform with potential applications in clinical diagnosis.
研究领域:
生物传感技术
·
医学诊断
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Bing zhang,Yu-lian Li,Li Wang,Haoyuan Cai
Conventional surface plasmon resonance (SPR) sensor often fails to detect low-concentration small molecules due to insufficient refractive index changes. To address this challenge, we have developed an enhanced SPR sensor utilizing Fe 3 O 4 @Au@polydopamine (PDA) core–shell magnetic nanoparticles as signal amplification tags for the detection of tebuconazole (TBZ). These nanoparticles improve sensitivity through their high refractive indices and enable TBZ enrichment from complex matrices via their magnetic properties. The sensor surface is further modified with PDA, which acts as a reducing agent for Au nanoparticle deposition, enhancing monoclonal antibody (Ab) immobilization and boosting the SPR signal. Our optimized sensor reaches a limit of detection (LOD) at 0.61 ng/mL for TBZ, with a linear response from 1-200 ng/mL, and demonstrates high selectivity and reproducibility, ideal for pesticide monitoring in food samples.
研究领域:
分析化学
·
食品科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
·
2023
·
IF 5.6
Liangliang Cheng,Wanlu Zheng,Ya-Nan Zhang,Xuegang Li,Yong Zhao
A highly sensitive fiber-optic urea sensor was designed and experimentally demonstrated based on surface plasmon resonance (SPR) effect and metal-organic zeolite framework (MOF), [zeolite imidazole skeleton-8 (ZIF-8)]/urease modification. The SPR effect was excited by plating gold film on the thin core fiber (TCF) in multimode-thin core-multimode fiber (MMF) structure, which behaved high refractive index sensitivity. The ZIF-8/urease composite film was grown on the gold film by rapid aqueous phase synthesis method, in which the ZIF-8 could immobilize more urease and made urease and urea fully contact. Since the urease catalyzed urea hydrolysis would change the refractive index, the measurement of urea concentration was realized with high sensitivity and good selectivity. Experimental results showed that the urea measurement sensitivity of the proposed sensor could reach 6 nm/mM within the concentration range of 1–7 mM, which has great application potential in the field of human health monitoring.
研究领域:
光学传感
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）