JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2018
·
IF 3.235
Qianrui Liu,Kefeng Ma,Dongxiao Wen,Qiangwei Wang,Haobo Sun,Qingyun Liu,Jinming Kong
In this work, a biosensor for ultrasensitive detection of glucose via electrochemically mediated atom transfer radical polymerization (eATRP) was firstly reported in the field of biosensing analysis. Firstly, 4-mercaptophenylboronic acid was immobilized onto Au electrode surface via Au S bond to recognize glucose by producing 5-membered cyclic boronic ester. Then initiators of atom transfer radical polymerization were introduced to the electrode surface by the reaction between 2-bromoisobutyryl bromide and the remaining hydroxyl groups of glucose to produce the alkyl halide initiation site. The monomer began to graft onto the electrode surface, and an electrochemical potential was applied to activate the copper catalyst for the formation of polymeric chains which were labeled numerous electroactive probes ferrocene . The square wave voltammetry was applied to monitor electrochemical signal of quantitatively labeled ferrocene. Under optimal conditions, this strategy for ultrasensitive detection of glucose presented a good linear relationship between oxidation current of ferrocene and logarithm of glucose concentrations in the range from 1.0 nM to 10 μM with an extremely low limit of detection of 0.32 nM (R 2 = 0.996). In addition, this strategy showed good specificity for glucose detection in physiological conditions and also showed high reliability in human serum.
研究领域:
生物传感
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分析化学
·
电化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Danlin Zhang,Shanshan Zhong,Chunlan Peng,Liuyu Chen,Jun Luo,Xinchun Li
Digoxin is a crucial cardiotonic agent, which has been widely used for remedy of heart failure for decades in clinical practice. Unfortunately, digoxin behaves extremely narrow therapeutic index and huge individual difference, therefore alerting its concentration level is imperative regarding therapeutic efficacy and safety. In this work, we initially present a label-free ultrasensitive electrochemical sensor for digoxin by dual signal amplification strategy integrating catalytic hairpin assembly (CHA) and electrochemically mediated atom transfer radical polymerization (eATRP). A DNA hairpin structure (H1) bearing azide group was firstly immobilized on gold electrode via Au-S bond. Then, an aptamer-containing initiator triggered CHA reaction with the assistance of hairpin-structured helper (H2), thereby exposing the azide group to enable the introduction of functional element for eATRP by click chemistry. The eATRP was electrochemically initiated, producing abundant electroactive species tethering to the formed H1-H2 hybrid structure. Through DNA-mediating electron transfer mechanism, a strong electrochemical current signal can be generated. The peak current measured by square wave voltammetry (SWV) was proportional to digoxin concentration ranging from 1.0 pM to 40 pM, with the detection limit of 0.59 pM, outperforming currently widely used ELISA, FPIA and LC-MS/MS. The designed electrochemical sensor was used for detection of clinical blood samples and the analytical results were in line with traditional immunoassay. By taking the advantages including low-cost, high sensitivity, and reliable assays, the present sensor offers a means for digoxin therapeutic monitoring. More importantly, this work would enrich our design strategy of electrochemical sensors toward clinical biopharmaceutics.
研究领域:
生物医学工程
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临床药理学
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分析化学
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DOI
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 22
·2827-2834
·
IF 2.596
Kai Hu,Jiamin Cheng,Jinge Li,Shan Ye,Huaixia Yang,Yanju Liu,Jinming Kong
The sensitive detection of biomarker cytokeratin fragment antigen 21-1 (CYFRA21-1) is crucial for early diagnosis and screening of non-small cell lung cancer (NSCLC). In this work, an electrochemical biosensor based on Nafion-initiated eATRP has been built for ultrasensitive detection of CYFRA21-1 DNA for the first time. Specifically, peptide nucleic acid (PNA) probes are immobilized onto a gold electrode surface and then hybridized with target DNA to form PNA/DNA heteroduplexes for the subsequent attachment of Nafion by the identified carboxyl–Zr4+–phosphoric acid chemistry. Finally, polymer chains are obtained by linking the monomer of ferrocenylmethyl methacrylate to the PNA/MCH/DNA/Zr4+/Nafion probes via eATRP. Under optimized steady-state conditions, the sensor offers a wide current response for CYFRA21-1 DNA from 10−11 to 10−16 M with a detection limit of 6.42 × 10−17 M. The proposed method of using Nafion as the eATRP initiator exhibits high sensitivity, reproducibility and stability and is a promising strategy for early diagnosis of NSCLC.
研究领域:
癌症诊断
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生物化学
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电化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Liying Zhao,Huaixia Yang,Xiaoke Zheng,Jinge Li,Lihe Jian,Weisheng Feng,Jinming Kong
Cytokeratin fragment antigen 21–1 (CYFRA 21–1) DNA is a crucial biomarker closely associated with non-small cell lung cancer. Here, we fabricated a novel electrochemical biosensor for ultrasensitive detection of CYFRA 21–1 DNA via polysaccharide and electrochemically mediated atom transfer radical polymerization (eATRP) dual signal amplification . Specifically, thiolated peptide nucleic acid (PNA) probes at 5′-terminals are immobilized on the gold electrode surface for specific recognition of CYFRA 21–1 DNA (tDNA). After hybridization, hyaluronic acid (HA) is linked to the hybridized PNA/DNA duplexes via the recognized carboxylate-Zr 4+ -phosphate chemistry . Then multiple initiators of the polymerization reaction are introduced via esterification reaction. Lastly, large numbers of electro-active monomers are successfully grafted from the initiation sites of functionalized HA by eATRP reaction, significantly amplifying the electrochemical signal. Under optimal conditions, the constructed sensor can detect as low as 9.04 aM tDNA. Further, this proposed biosensor can also be applied to the direct detection of double-stranded DNA (dsDNA), obtaining 0.12 fM as the detection limit. Besides, this strategy shows high selectivity for mismatched bases and excellent applicability for CYFRA 21–1 DNA detection in the serum samples. Given its high sensitivity, selectivity, ease of operation, low cost and environmental friendliness , this biosensor has considerable potential in early diagnosis and biomedical application .
研究领域:
生物化学
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癌症研究
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分析化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 19
·5691-5699
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IF 4.019
Manman Li,Zhuangzhuang Guo,Xiaoke Zheng,Huaixia Yang,Weisheng Feng,Jinming Kong
Herein, a novel aptasensor was constructed for ultrasensitive detection of bisphenol A (BPA). In this method, an electrochemically mediated atom transfer radical polymerization (eATRP) signal amplification strategy was applied to BPA detection for the first time. The 5′-end modified sulfhydryl group and the 3′-end modified azide group hairpin DNA were immobilized on a gold electrode through an Au–S bond. The double-stranded DNA was formed by the hybridization of an aptamer and a single-stranded DNA partially paired with the hairpin DNA. In the presence of BPA, the aptamer combined with BPA and the single-stranded DNA was released to open the hairpin structure, making the azide groups at the 3′ end exposed. Subsequently the initiator of eATRP was introduced into hairpin DNA through click chemistry reaction and eATRP was conducted for the polymerization of the electroactive probe ferrocene methyl methacrylate (FMMA). As a result, the ultrasensitive detection of BPA was realized, and the detection limit of this aptasensor was as low as 59 aM and a good selectivity was obtained in the presence of 100-fold structural analogs. The application of this aptasensor was evaluated by detecting BPA in pure water samples, and recoveries were in the range of 95.23–98.40%, holding promising applications in biological analysis.
研究领域:
分析化学
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环境科学
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电化学
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纳米技术
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生物技术
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DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2020
·Vol. 32
·期 8
·1772-1779
·
IF 2.544
Yifei Yang,Yue Sun,Mingzhu Jin,Ru Bai,Yutong Liu,Yingqi Wu,Wei Wang,Xuewei Feng,Siyu Li
Superoxide dismutase (SOD) plays an important role in nearly all living cells. In this study, SOD imprinted poly(ionic liquid)s (SIPILs) were prepared on the surface of the bare Au electrode modified with nano-palladium and nano-gold (Au/nPd/nAu/SIPILs). SIPILs was synthesized with 1-vinyl-3-propyl imidazole sulfonate ionic liquids as functional monomers via electrochemically mediated atom transfer radical polymerization (eATRP) catalyzed by SOD. The Au/nPd/nAu/SIPILs was examined by cyclic voltammetry (CV), scanning electron microscope (SEM), energy-dispersive spectrometer (EDS) and X-ray photoelectron spectroscopy (XPS). The Au/nPd/nAu/SIPILs was also used as an electrochemical sensor to determine SOD by differential pulse voltammetry (DPV). Under the optimal conditions, the detection range of SOD was from 1.0×10 −8 to 1.0×10 2 mg L −1 with a limit of detection of 8.90×10 −9 mg L −1 (S/N=3). Compared with other methods, the sensor based on SIPILs had the broader linear range and lower detection limit.
研究领域:
分析化学
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材料科学
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电化学
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生物传感器
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Tingting Ding,Ruixia Liu,Xiaofan Yan,Zuoyu Zhang,Fuquan Xiong,Xingong Li,Zhiping Wu
It is a promising idea to graft zwitterionic polymers onto lignin and prepare lignin-grafted-poly [2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide (Lignin-g-PDMAPS) thermosensitive polymer with the upper critical solution temperature (UCST). In this paper, an electrochemically mediated atom transfer radical polymerization (eATRP) method was used to prepare Lignin-g-PDMAPS. The structure and property of the Lignin-g-PDMAPS polymer were characterized by the fourier transform infrared spectrum (FT-IR), nuclear magnetic resonance (NMR), X-ray electron spectroscopy (XPS), dynamic light scattering (DLS), differential scanning calorimeter (DSC). Furthermore, the effect of catalyst structure, applied potential, amount of Lignin-Br, Lignin-g-PDMAPS concentration, NaCl concentration on UCST of Lignin-g-PDMAPS were investigated. It was worth noting that polymerization was well controlled when the ligand was tris (2-aminoethyl) amine (Me 6 TREN), applied potential was −0.38 V and the amount of Lignin-Br was 100 mg. And the UCST of the Lignin-g-PDMAPS aqueous solution (1 mg/ml) was 51.47 °C, the molecular weight was 8987 g/mol, and the particle size was 318 nm. It was also found that the UCST increased and the particle size decreased with the Lignin-g-PDMAPS polymer concentration increased, and the UCST decreased and the particle size increased with the NaCl concentration increases. This work investigated UCST-thermoresponsive polymer which possessed lignin main chain combining the zwitterionic side chain, and provided a new way for development of lignin based UCST-thermoresponsive materials and medical carrier materials, in addition to expand the scope of eATRP.
研究领域:
材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER SCIENCE
·
2023
·Vol. 61
·期 12
·1150-1161
·
IF 3.4
Liu Yan,Lan Xu
A novel amphiphilic photoreactive block copolymer was synthesized by electrochemically mediated atom transfer radical polymerization (eATRP) of glycidyl methacrylate (GMA), N -vinyl carbazole (VK) with polyethylene glycol-based initiator (PEG-Br). And then the double bond and the boric acid group were introduced through the epoxy group. Fluorescent molecularly imprinted nanoparticles (nano-FMIPs) were prepared using self-assembly imprinting technology with PEG-b-P(GMA-co-VK) as a functional monomer and sialic acid (SA) as a template molecule. The nano-FMIPs prepared by this method have the advantages of uniform size and small particle size, which greatly improves their recognition performance. It is found that the SA template can significantly quench the fluorescence intensity of nano-FMIPs linearly within a concentration range of 4 × 10 −4 –4 × 10 −6 mol L −1 , which is well described by Stern–Volmer equation. The nano-FMIPs can selectively and sensitively detect down to 2.9 μM of SA concentration. This study provides a new and general strategy for the preparation of nano-MIPs with high selective recognition ability in aqueous media, which has good application prospects in biomedicine. Graphical
研究领域:
材料科学
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生物医学工程
·
分析化学
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产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2024
·Vol. 141
·期 13
·e55146
·
IF 2.7
Qin Liu,Lan Xu
In this study, the gold nanoparticles (Au NPs) are capped with a novel, well-defined double hydrophilic block copolymer, poly (ethylene glycols)-block-poly(mono-2-(methacryloyloxy) ethyl succinate) (PEG-b-PSEMA). The PEG-b-PSEMA, with long side chains and relatively narrow dispersity index, is successfully prepared by aqueous electrochemically mediated atom transfer radical polymerization using a PEG-Br macroinitiator (M n ≈2000 g mol −1 ). By simply mixing PEG-b-PSEMA and Au precursor before adding reductants, coulombic interactions occur between the Au precursor and the carboxylate group of the PSEMA, as a result dense Au NPs are formed. This enables the Au NPs to remain dispersed even in the presence of temperature variations, pH adjustments, and incubation in organic solvents. Au NPs were endowed by PEG-b-PSEMA with more remarkable catalytic activity in organic solvents of 4-nitrophenol into 4-aminophenol than by sodium citrate (Au@citrate) and MeO-PEG-OH (Au@PEG). In addition, PEG-b-PSEMA-stabilized Au NPs (Au@PEG-b-PSEMA) were also tested for the dye degradation property against Rhodamine-B in only pH 10 basic solution, and over 99% of dye degradation can be achieved in 9 min. PEG-b-PSEMA induces coulombic interactions with Au NPs, providing outstanding stability for catalytic applications in organic or specific acidic-base environments.
研究领域:
材料科学
·
化学
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产品关联
DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 14
·B1251
·
IF 3.12
Yue Sun,Yifei Yang,Siyu Li,Wei Wang,Xuewei Feng,Mengyuan Zhao,Yutong Liu
Myoglobin (Mb) imprinted polymer was synthesized via electrochemically mediated atom transfer radical polymerization (eATRP) catalyzed by Mb on the surface of Au electrode which had been modified with three-dimensional foam graphene (3DG). The electrode modified with protein imprinted polymers (Au/3DG/PIPs) was examined by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Furthermore, the Au/3DG/PIPs electrode was used as electrochemical biosensor and to determine Mb by differential pulse voltammetry (DPV). Under the optimal conditions, the experimental results showed highly selectivity with linear relationships from 1.0 × 10−10 to 1.0 × 10−1 mg·L−1, and a limit of detection of 1.10 × 10−11 mg·L−1 (S/N = 3). Compared with similar sensors, the broader linear range and lower detection limit suggested the promising prospect of this biosensor.
研究领域:
材料科学
·
分析化学
·
纳米技术
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2018
·
IF 3.927
Dan Li,Xiaoqin Niu,Shiyuan Yang,Yuhong Chen,Fen Ran
We graft thermo-responsive polymer chains of PNIPAm, random polymer chains of P(NIPAm- co -Am), and P(NIPAm- co -GMA) on the surface of polyethersophone membrane via SI-eATRP method. The polymer chains of PNIPAm are thermo-responsive segment, and PAm and PGMA are hydrophilic and hydrophobic segment, respectively, which endow the modified membrane thermo-responsive property and adjust the lower critical solution temperature (LCST) of the modified membrane. The influence of temperature on membrane permeation and fouling process are investigated. The LCST is determined by filtration experiment at different temperature. The results show that all modified membranes have thermo-responsive behavior, and LCST of M-PNIPAm- co -PAm increased to 38 °C compared with M-PNIPAm; but M-PNIPAm- co -PGMA has the same LCST with M-PNIPAm. The modified membranes have higher flux than that of pristine polyethersulphone membrane whether feeding with pure water or BSA solution, which mainly owing to the hydrophilic surface and rich porosity of modified membranes.
研究领域:
材料科学
·
环境工程
✓ 已验证
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Yue Sun,Siyu Li,Yifei Yang,Xuewei Feng,Wei Wang,Yutong Liu,Mengyuan Zhao,Zhiguang Zhang
A new kind of thermal responsive protein imprinted polymers (TPIPs) was prepared for the electrochemical biosensor. The TPIPs were synthesized on the surface of zinc oxide (ZnO) nanoflowers via electrochemically mediated atom transfer radical polymerization (eATRP), with bovine hemoglobin (Hb) as both catalyst and template. N-isopropyl acrylamide (NIPAM) and methacrylic acid (MAA) were used as temperature sensitive and assistant functional monomer , respectively. The biosensor fabricated by TPIPs and ZnO was successfully used for detecting Hb by differential pulse voltammetry (DPV). Under suitable conditions, the detection range of Hb was from 1.0 × 10 −13 to 1.0 × 10 −1 mg·L −1 with a low detection limit of 3.1 × 10 −14 mg·L −1 (S/N = 3). This biosensor was used for detecting bovine hemoglobin in real samples with the recoveries of 95–105%. The good temperature response, good selectivity and repeatability allowed the TPIPs to be a great importance for the polymer-modified electrodes and the sensitive detection of Hb.
研究领域:
生物传感器
·
电化学
·
纳米技术
·
高分子科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）