JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2021
·
IF 3.205
Yuan Zhou,Mengying Wei,Meiling Fan,Zhongying Liu,Aimin Wang,Yuanyuan Liu,Lihui Men,Zifeng Pi,Zhiqiang Liu,Fengrui Song
Lignans from Schisandra chinensis (Turcz.) Baill (LFS) has been proved to improve impaired cognitive ability thereby show potential in treating Alzheimer’s disease (AD). In this study, UHPLC-Q-TOF-MS and UHPLC-QQQ-MS were adopted cooperatively to establish a method synchronously detecting 10 kinds of LFS monomers in rat plasma samples. And this method was further applied for pharmacokinetic study to compare the metabolism of LFS in normal and AD rats. The results indicated that AD rats showed an observably better absorption of LFS compared to normal rats. Based on time-varying plasma concentration of LFS, metabolomics was used to establish a plasma concentration–time-endogenous metabolite connection. In total 54 time-varying endogenous metabolites were screened and most of which were closely associated with AD. And LFS exerted a concentration dependent regulating effect to most of these metabolites. Through biomarker related pathways and biological function analysis, LFS might treat AD through neuroprotection, antioxidant damage and regulating the metabolism of unsaturated fatty acids. This is the first study connecting LFS absorbtion and endogenous metabolite changes with the time lapse. The pharmacokinetics and metabolic profile differences between normal and AD rats were firstly investigated as well. This study provides a novel perspective in exploring the effect and mechanism of LFS in treating AD.
研究领域:
神经科学
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药物代谢研究
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中药学
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产品关联
DOI
PubMed
高影响因子
Food & Function
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2019
·Vol. 10
·期 9
·5656-5668
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IF 3.241
Mengying Wei,Zhongying Liu,Yuanyuan Liu,Shizhe Li,Mingxin Hu,Kexin Yue,Tianshu Liu,Yang He,Zifeng Pi,Zhiqiang Liu,Fengrui Song
Schisandra chinensis (Turcz.) Baill (S. chinensis), a functional food, is used as a tonic and sedative agent in traditional Chinese medicine. Modern pharmacological research has proved that S. chinensis could prevent and treat age-related neurodegenerative diseases. The presence of bioactive lignans in S. chinensis is the main reason for its neuroprotective and cognitive enhancement effects. This study aimed to clarify the mechanism of lignans in S. chinensis in ameliorating learning and memory deficits in Alzheimer's disease (AD) animals. The step-down test and Morris water maze (MWM) test were used to verify the effects of lignans in S. chinensis on learning and memory in AD animals. Then, metabolomics approaches based on ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) were used to clarify the mechanism of lignans in S. chinensis in treating AD. Finally, quantitative analysis of AD-related neurotransmitters in the brain was conducted after treatment with lignans in S. chinensis. In the MWM and step-down tests, lignans in S. chinensis showed a clear ability to ameliorate the impaired learning and memory of AD animals. A total of 31 endogenous metabolites were identified after treatment with lignans in S. chinensis, which were associated with lignans ameliorating learning and memory. These biomarkers were mainly associated with polyunsaturated fatty acid metabolism and amino acid and vitamin metabolism. Moreover, lignans in S. chinensis upregulated the levels of γ-aminobutyric acid (GABA), 5-hydroxytryptamine (5-HT), acetylcholine (Ach), norepinephrine (NE) and glycine (Gly) and downregulate the level of aspartic acid (Asp). Lignans in S. chinensis might alleviate the neurotoxic effects of neurological inflammation and oxidative stress, Aβ deposition, and tau phosphorylation via the regulation of multiple endogenous metabolic pathways during pathological AD. The research might provide useful support for the further study of pharmacology and new drug development of lignans in S. chinensis.
研究领域:
药理学
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神经科学
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产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
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2016
·
IF 2.408
Ye Liu,Yan-Mei Wang,Wen-Ming Wu,Jian Song,Han-Li Ruan
Three new ( 1–3 ) and seven known ( 4–10 ) triterpenoids , together with one new ( 11 ) and fifteen known ( 12–26 ) dibenzocyclooctadiene lignans , were isolated from the fruit of Schisandra sphenanthera Rehd. et Wils. Their structures were elucidated by extensive analysis of spectroscopic methods, including HRESIMS, 1D and 2D NMR spectra and CD experiments. All of the isolated triterpenoids ( 1 − 10) were evaluated for their in vitro cytotoxicities against HepG2 human hepatocellular carcinoma cell line, and compounds 1 , 3–7 and 10 exhibited moderate antiproliferative effects against HepG2 cell line with IC 50 ranging from 18.12 to 49.52 μM. The lignans ( 11–26 ) were tested for their neuroprotective activities against CoCl 2 , H 2 O 2 and A β 25–35 -induced SH-SY5Y cell injuries and showed considerable neuroprotective effects of different degrees. And at the low concentration of 3.2 nM, compounds 14 , 17–19 , 23 in CoCl 2 -induced, compounds 11 , 13–15 , 17 , 19–20 , 22–24 in H 2 O 2 -induced, and compounds 12–14 , 19 , 25–26 in A β 25–35 -induced SH-SY5Y cell injury models, showed statistically significant neuroprotective activities compared with the negative control group, respectively.
研究领域:
药理学
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植物化学
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神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
·
2025
·
IF 2.6
Jinyuan Yang,Zhaojing Chen,Yikun Ren,Chunlei Yuan,Senhua Hu,Yiyang Liu,Dahong Li,Huiming Hua
Phytochemical investigation of Thalictrum baicalense Turcz. roots, traditionally used for inflammatory conditions, led to the isolation of three novel isoflavones, thalibaicalins A-C ( 1 – 3 ), and a new lignan, (7 R , 8 S , 7′ R , 8′ S )-9 E -feruloylneoolivill ( 6 ), alongside six known compounds ( 4 – 5 , 7 – 10 ). Structural characterization was accomplished by HRESIMS, comprehensive NMR analysis, and quantum chemical calculations. Anti-inflammatory evaluation in lipopolysaccharide-stimulated RAW 264.7 macrophages identified potent NO inhibitors, with lignan 6 exhibiting the highest activity (IC 50 = 9.56 ± 2.03 μM). This study expands the phytochemical diversity of T. baicalense Turcz. and highlights its potential as a source of anti-inflammatory lead compounds, particularly the highly active lignan derivative.
研究领域:
植物化学
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药理学
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药物化学
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天然产物研究
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炎症生物学
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产品关联
DOI
PubMed
高影响因子
Acta Pharmaceutica Sinica B
·
2023
·
IF 14.5
Lijuan Xue,Keanqi Liu,Caixia Yan,Junling Dun,Yexin Xu,Linlin Wu,Huizhu Yang,Huafang Liu,Lin Xie,Guangji Wang,Yan Liang
Nonalcoholic steatohepatitis (NASH) is a spectrum of chronic liver disease characterized by hepatic lipid metabolism disorder. Recent reports emphasized the contribution of triglyceride and diglyceride accumulation to NASH, while the other lipids associated with the NASH pathogenesis remained unexplored. The specific purpose of our study was to explore a novel pathogenesis and treatment strategy of NASH via profiling the metabolic characteristics of lipids. Herein, multi-omics techniques based on LC–Q-TOF/MS, LC–MS/MS and MS imaging were developed and used to screen the action targets related to NASH progress and treatment. A methionine and choline deficient (MCD) diet-induced mouse model of NASH was then constructed, and Schisandra lignans extract (SLE) was applied to alleviate hepatic damage by regulating the lipid metabolism-related enzymes CES2A and CYP4A14. Hepatic lipidomics indicated that MCD-diet led to aberrant accumulation of phosphatidylethanolamines (PEs), and SLE could significantly reduce the accumulation of intrahepatic PEs. Notably, exogenous PE (18:0/18:1) was proved to significantly aggravate the mitochondrial damage and hepatocyte apoptosis. Supplementing PE (18:0/18:1) also deteriorated the NASH progress by up regulating intrahepatic proinflammatory and fibrotic factors, while PE synthase inhibitor exerted a prominent hepatoprotective role. The current work provides new insights into the relationship between PE metabolism and the pathogenesis of NASH.
研究领域:
药理学
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肝病学
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脂质组学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
Separation Science Plus
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2024
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IF 1.3
Boyu Chen,Shengkai Liu,Hongxin Zhi,Jin Huang,Han Song,Jiashen Fan,Yujie Fu,Zhiguo Liu
Green extraction of the active lignans from Schisandra chinensis has received considerable attention due to their promising applications in the biomedical field. In this study, microwave-assisted deep eutectic solvent method was used to simultaneously extract five lignans: Schizandrol A, Schizandrol B, Schisantherin A, Schisandrin A and Schizandrin B. The extraction parameters were optimized according to the calculated comprehensive score obtained from the analytic hierarchy process combined with the entropy weight method. The optimal extraction conditions were that the molar ratio of choline chloride and glycolic acid is 4:1, water content is 30% (V/V), solid–liquid ratio is 1:20 (W g :V mL ), extraction time is 20 min, and extraction temperature is 70°C, under which the extraction rate of the five lignans were 10.89, 8.616, 4.019, 4.893, and 5.318 mg/g. These results confirmed that our extraction method is efficient and feasible for green extraction of lignans from S. chinensis .
研究领域:
药学
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分析化学
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绿色化学
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DOI
高影响因子
FITOTERAPIA
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2020
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IF 2.527
Xiu-Qing Song,Jia Sun,Jin-Hai Yu,Jun-Sheng Zhang,Jie Bao,Hua Zhang
Six new compounds including four prenylated indole alkaloids ( 1 – 4 ) and two lignans ( 5 – 6 ), along with eight known cometabolites ( 7 – 14 ), were isolated from the flower buds of Tussilago farfara . Structures of the new compounds were elucidated by comparison with structurally related known analogues and also by comprehensive spectroscopic analyses. Their absolute configurations were determined by a variety of means including Mosher's method, Marfey's analysis, electronic circular dichroism (ECD) exciton chirality method and ECD calculations. Our bioassays have established that compounds 1 and 2 showed potent α -glucosidase inhibitory activity with IC 50 values of 105 ± 4.7 and 35.2 ± 3.2 μM, respectively, while the known 13 and 14 exerted moderate DPPH radical scavenging activity with IC 50 values of 45.2 ± 2.9 and 29.2 ± 2.0 μM, respectively.
研究领域:
植物化学
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天然产物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NATURAL PRODUCTS
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2014
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IF 3.947
Heng-Yi Yu,Zu-Yu Chen,Bin Sun,Junjun Liu,Fan-Yu Meng,Ye Liu,Tian Tian,An Jin,Han-Li Ruan
Two rare 7,8-seco-lignans (1, 2), three new lignan glycosides (3, 4a, 4b), and 10 known lignans (5–14) were isolated from the fruit of Schisandra glaucescens Diels. The absolute configurations of 1 and 2 were determined by comparing their experimental and calculated electronic circular dichroism spectra. The molecular structures of the new compounds (3, 4a, and 4b), including their absolute configurations, were determined using various spectroscopic methods and hydrolysis reactions. The antioxidant activities of the isolated compounds were tested using 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power assays. Compounds 4, 7, 8, 10, 11, and 12 exhibited antioxidant activities of varying potential in both assays. Of these compounds, 7 showed the strongest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, with IC50 values of 15.7 (150 μM DPPH) and 34.6 μM (300 μM DPPH), respectively, and 4, 12, and 7 displayed higher total antioxidant activities than Trolox in the ferric reducing antioxidant power assay. The neuroprotective effects of these compounds against Aβ25–35-induced cell death in SH-SY5Y cells were also investigated. Compounds 1, 2, 6, 7, 8, 11, and 12 exhibited statistically significant neuroprotective effects against Aβ25–35-induced SH-SY5Y cell death compared with the group treated only with Aβ25–35.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2013
·
IF 2.906
Jia Sun,Jin Yu,Pei-Cheng Zhang,Feng Tang,Yong-De Yue,Ya-Nan Yang,Zi-Ming Feng,Xue-Feng Guo
Caulis Bambusae in Taenia is a medicinal preparation from Bambusa tuldoides Munro consisting of skinless slices of the stem (bamboo shavings) and used as a traditional health food in tea, wine, and soup in Asia. Three novel lignans, (−)-7′-epi-lyoniresinol 4,9′-di-O-β-d-glucopyranoside (7), (−)-lyoniresinol 4,9′-di-O-β-d-glucopyranoside (8), bambulignan A (10), and seven known lignan compounds (1–6 and 9) were isolated from Caulis Bambusae in Taenia. The structures of the lignans were determined by detailed spectroscopic analysis (HRESIMS, HSQC, HMBC, NOE). All the isolated lignans were tested for antioxidant activities by DPPH and FARP assays. The results showed that the compounds (+)-lyoniresinol 9′-O-β-d-glucopyranoside (1) and (−)-7′-epi-lyoniresinol 9′-O-β-d- glucopyranoside (9) have strong free radical scavenging activity and reducing power.
研究领域:
药用植物学
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天然产物化学
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药物化学
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产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
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2018
·
IF 3.929
Qingbo Liu,Jie Wang,Bin Lin,Zhuo-Yang Cheng,Ming Bai,Shaochun Shi,Xiao-Xiao Huang,Shao-Jiang Song
Alzheimer’s disease (AD) is characterized by the progressive accumulation of extracellular β -amyloid (A β ) aggregates. Recently, lignans and phenylpropanoids are attracting increasing attention to discovery useful agents of inhibition on A β aggregation. In the present study, to develop potential agents for slowing the progression of AD, Prunus tomentosa seeds were selected as a raw material for bioactive compounds, which led to the separation of two pairs of new enantiomeric lignans and phenylpropanoids using chiral HPLC. The planar structures of these compounds were elucidated by spectroscopic data analyses. And their absolute configurations were determined by comparing of experimental and calculated electronic circular dichroism (ECD). The biosynthesis pathway was also discussed. Additionally, the inhibitory activity on A β aggregation of all optical pure compounds was tested by thioflavin T (ThT) assay. The isolates ( 1a , 1b , 2a and 2b ) showed more potent inhibitory activity than positive control curcumin with inhibitory rate of 73.89 ± 3.41% 78.69 ± 1.50%, 63.25 ± 2.68%, and 67.13 ± 0.90% at 20 μM, respectively. More importantly, the inhibition profiles were explained by molecular dynamics and docking simulation studies.
研究领域:
药理学
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植物化学
·
神经科学
✓ 已验证
DOI
PubMed
高影响因子
PHYTOCHEMISTRY
·
2024
·
IF 3.2
Hao-Dong Yang,Yuan-Gui Yang,Zhi-Shu Tang,Kang Ma,Hong-Bo Xu
Acanthopanacis cortex (the dried root bark of Acanthopanax gracilistylus W. W. Smith) has been used for the treatment of rheumatic diseases in China for over 2000 years. Four previously undescribed lignans ( 1 – 4 ) and 12 known lignans ( 5 – 16 ) were isolated from Acanthopanacis cortex. In this study, the inhibitory activities of compounds 1 – 16 against neutrophil elastase (NE), cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) are reported. The results show that compounds 1 – 16 exhibit weak inhibitory activities against NE and COX-1. However, compounds 2 , 6 – 8 and 13 – 16 demonstrate better COX-2 inhibitory effects with IC 50 values from 0.75 to 8.17 μ Μ. These findings provide useful information for the search for natural selective COX-2 inhibitors.
研究领域:
药理学
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中药学
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天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 24
·15482
·
IF 6.208
Won Young Jang,Mi-Yeon Kim,Jae Youl Cho
Since chronic inflammation can be seen in severe, long-lasting diseases such as cancer, there is a high demand for effective methods to modulate inflammatory responses. Among many therapeutic candidates, lignans, absorbed from various plant sources, represent a type of phytoestrogen classified into secoisolariciresionol (Seco), pinoresinol (Pino), matairesinol (Mat), medioresinol (Med), sesamin (Ses), syringaresinol (Syr), and lariciresinol (Lari). Lignans consumed by humans can be further modified into END or ENL by the activities of gut microbiota. Lignans are known to exert antioxidant and anti-inflammatory activities, together with activity in estrogen receptor-dependent pathways. Lignans may have therapeutic potential for postmenopausal symptoms, including cardiovascular disease, osteoporosis, and psychological disorders. Moreover, the antitumor efficacy of lignans has been demonstrated in various cancer cell lines, including hormone-dependent breast cancer and prostate cancer, as well as colorectal cancer. Interestingly, the molecular mechanisms of lignans in these diseases involve the inhibition of inflammatory signals, including the nuclear factor (NF)-κB pathway. Therefore, we summarize the recent in vitro and in vivo studies evaluating the biological effects of various lignans, focusing on their values as effective anti-inflammatory agents.Keywords:lignans;antioxidant;chronic inflammation;menopausal symptoms;cancers
研究领域:
药理学
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免疫学
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肿瘤学
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内分泌学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）