RESPIRATORY RESEARCH
·
2025
·Vol. 26
·期 1
·1-17
·
IF 5
Ren Shencheng,Zhu Junkan,Shan Guangyao,Liang Jiaqi,Bian Yunyi,Lin Han,Shi Haochun,Pan Binyang,Zhao Guangyin,Yang Huiqin,Huang Xiaolong,Zhan Cheng,Ge Di,Bi Guoshu
Background Lung cancer remains the leading cause of cancer-related mortality globally, with lung adenocarcinoma (LUAD) being the most prevalent subtype. Despite extensive research efforts, the role of transcription factors in LUAD progression remains largely uncharacterized. In this study, we focused on ZNF266, a transcription factor whose impacts on LUAD have not been investigated. Methods Using high-throughput sequencing data, we observed a significant downregulation of ZNF266 expression in LUAD tissues. To validate this finding, we conducted a retrospective analysis of nearly three thousand LUAD patients’ data from public databases and our institution. Functional studies were performed using cell lines, organoids, and xenograft models to assess the role of ZNF266 in LUAD progression. RNA sequencing, chromatin immunoprecipitation, DNA pull-down assays, and dual-luciferase reporter assays were employed to elucidate the underlying mechanism. Additionally, adeno-associated virus (AAV)-mediated overexpression of ZNF266 was used to evaluate its therapeutic potential. Results Patients with low ZNF266 expression had poorer prognosis compared to those with high expression. ZNF266 inhibits the malignant phenotypes of LUAD, including proliferation, migration, and invasion. Mechanistically, ZNF266 binds to the promoter region of CA9, suppressing its transcription. This leads to a reduction in intracellular pH and subsequent inhibition of the mTOR signaling pathway, which is crucial for cancer cell growth and survival. Furthermore, AAV-mediated overexpression of ZNF266 significantly inhibited tumor growth in patient-derived xenograft models. Conclusions Our study demonstrated that ZNF266 inhibits LUAD progression in a pH-dependent manner via modulating CA9 expression, uncovering its therapeutic significance for LUAD treatment.
研究领域:
肿瘤学
·
分子生物学
·
医学遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR MATERIALS AND ENGINEERING
·
2023
·Vol. 308
·期 11
·2300141
·
IF 3.9
Seojin Jung,Seokhee Chang,Na-Eun Kim,Yoon-Jae Song,Jooyoun Kim
A wound evolves with variable pHs depending on the healing stage, and the maintenance of mild acidic condition can help healing by deterring the formation of a biofilm. Herein, a dual-layer wound dressing with an acid-responsive zeolitic imidazolate framework–8 (ZIF-8) layer and an acid-modulating layer is designed to control the acid-dependent release of therapeutic agents for the improved healing process. The outer dressing layer is coated with curcumin-incorporated ZIF-8, where curcumin and zinc ions are released upon dissociation of ZIF-8 in an acidic condition. The inner layer, a pH modulator, is fabricated by electrospinning polyvinylpyrrolidone with addition of acetic acid. In vitro release test shows that the outer layer dressing attains a considerably higher release of curcumin and Zn 2+ under an acidic condition. Combined with the inner acidic layer, the dual layer dressing exhibits facilitated release of effective agents regardless of environmental pHs, attributed to the proper pH modulation to about 6.5. The anti-oxidant activity of curcumin and antibacterial activity of Zn 2+ are confirmed. The study highlights the novel proof-of-concept design of active wound dressing demonstrating the dual functions of pH regulation and the stimulus-responsive drug delivery.
研究领域:
材料科学
·
生物医学工程
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
Surfaces and Interfaces
·
2025
·
IF 6.3
Yajie Cheng,Zichen Xu,Qiang Wang,Kexin Zhang,Lin Wang,Yongbo Deng,Teng Ma,Hao Wei,Zengxian Guo
To address the issues of high filler content and poor wave absorption performance in single-component absorbers, we constructed tubular high-dielectric polypyrrole (PPy) through morphology control and doping, achieving high-performance microwave absorption at ultra-low filler content. This study utilized various surfactants for the oxidative polymerization of high-dielectric PPy (PPy-S X ) and its micromorphology was further optimized via pH regulation to further obtain excellent impedance matching of PPy (PPy-S 5 -X). Experimental results reveal that non-ionic surfactants, particularly methyl orange (MO), significantly improve the dielectric properties of tubular PPy (PPy-S 5 ). pH-regulation (from pH 1 to 11) markedly influences micromorphology. PPy-S 5 –5 (pH=5.0) possesses the most remarkable nanoparticle-nanotube morphology for impedance matching at ultralow filling content. With a thickness of 2 mm, PPy-S 5 –5 (5 wt% filling amount) exhibits an RL min of –40.88 dB with an effective absorption bandwidth of 5.04 GHz (10.24–15.28 GHz). By contrast, the RL min of PPy-S 5 –5 is over six times better than that of PPy-S 1 synthesized using cetyltrimethyl ammonium bromide (CTAB). This superior microwave absorption performance is due to enhanced electromagnetic wave loss for two main reasons: First, MO as a soft template promotes tubular PPy formation, allowing charges to continuously move and transfer between molecules to enhance conduction loss. Additionally, pH regulation optimizes particle distribution on PPy nanotubes, improving multi-interface interactions and interfacial polarization. This micromorphology regulation strategy of PPy unlocks its potential as a lightweight, efficient absorbent material, achieving superior absorption performance at low filling volumes in wave-absorbing applications.
研究领域:
材料科学
·
电磁学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiu-ning Yao,Rui-ling Dong,Yu-cong Li,Ao-jing Lv,Li-ting Zeng,Xue-qing Li,Zhou Lin,Jun Qi,Chun-hui Zhang,Guo-yuan Xiong,Qing-yong Zhang
The effects of pH-shifting treatments (pH 3, 5, 7, 9, and 11) on the stability of gelatin emulsions made by low-energy stirring were investigated. pH-shifting treatments significantly enhanced the ESI and EAI of the emulsion ( P < 0.05) and reduced its particle size ( P < 0.05) under low-energy emulsifying conditions. The pH 11 – 7 shifting treatment significantly increased the degree of depolymerization and the level of ordered structure of gelatin ( P < 0.05). These transformations resulted in a significant increase in the exposure of hydrophobic and negatively charged residues ( P < 0.05) on the surface of gelatin, facilitating a faster adsorption rate of gelatin onto the oil-water interface as well as an increase in the amount of gelatin adsorbed at the interface. Moreover, the alkali-shifting treatment promoted the formation of a thin viscoelastic interfacial film, which contributed to the enhanced stability of the emulsion.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Guohua Li,Xianjin Qi,Nina Yang,Xiaoxu Duan,Aimin Zhang
Molecular sieves have also been used for arsenic adsorption in recent years because of their special structure. In order to solve the problem of arsenic pollution in drinking water and/or industrial wastewater , ZSM-5/Fe adsorbent was prepared by loading iron on ZSM-5 molecular sieve. It is also used as an excellent adsorbent for removing arsenic and other heavy metal ions from industrial wastewater. At room temperature, the concentration of arsenic was reduced from 100 mg/L to 0.006 mg/L after the solution pH was adjusted to the range of weak acid to weak base (4–10) and 0.5 g of ZSM-5/Fe adsorbent was added for reacting 2 h. The adsorption capacity reached 40.00 mg/g, the adsorption efficiency reached 99.99%, reaching the national standard of drinking water. Adsorption thermodynamics, kinetics and isotherms showed that the adsorption mechanism of arsenic is heterogeneous nucleation adsorption (including electrostatic attraction and chemical precipitation). Moreover, ZSM-5/Fe adsorbent can adjust pH spontaneously by using non-skeleton Si–Al phase to achieve effective adsorption from weak acid to weak base. At the same time, ZSM-5/Fe adsorbent showed good reusability and stability in five cycles. This study provides an important idea for the application of ZSM-5 molecular sieve in many fields and the efficient removal of arsenic from drinking water and industrial wastewater.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECS Journal of Solid State Science and Technology
·
2024
·Vol. 13
·期 5
·054001
·
IF 1.8
Changxin Dong,Xinhuan Niu,Jianghao Liu,Ni Zhan,Yida Zou,Chao He,Xinjie Li,Fu Luo,Yunhui Shi,Jiabao Cheng
For Ruthenium (Ru)-based copper (Cu) interconnects Cu film chemical mechanical polishing (CMP), it is crucial to select appropriate pH regulators in the slurry to ensure the chemical reactions and maintain the stability of the polishing chemical environment. In this study, the effects of inorganic pH regulator KOH, organic pH regulator diethanolamine (DEA), and 2-amino-2-methyl-1-propanol (AMP) on CMP and slurry properties of Cu film were compared. It was found when using AMP as a pH regulator, the Cu/Ru removal rate selectivity (RRS) can reach 598:1, the surface roughness of Cu film decreased to 0.76 nm, and the slurry can remain stable for at least 7 d. The performance order of the three pH regulators is AMP＞KOH＞DEA. Meanwhile, through experimental results and test analysis, it has been confirmed that AMP can also play a multifunctional role as a complexing agent, dispersant, and surfactant. Therefore, AMP can replace KOH as a new pH regulator in weak alkaline slurries. This result plays an important role in guiding the selection of organic pH regulators in the optimization of Cu film CMP slurry.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Mingxue Xia,Xiao Meng,Limin Lin,Ang Gao,Yunhe Diao,Xuying Liu,Huige Yang
Hydrogel-based flexible electronic devices have garnered significant attention due to their excellent mechanical properties, high electrical conductivity, and signal sensitivity. Nevertheless, internal water molecules crystallize inevitably at low temperatures, impairing the performance of hydrogels. Designing anti-freezing and tough hydrogels to meet long-term stability requirements is extremely challenging. A double physically crosslinked PVA/SA-g-DA/Fe 3+ hydrogel was fabricated using a two-step method. The coordination mode between catechol groups and ferric ions was modified by adjusting pH of soaking solution, subsequently regulating antifreeze performance and mechanical properties of the hydrogels. The obtained PVA/SA-g-DA/Fe 3+ hydrogel is stretchable, tough, and has a remarkable freeze tolerance (−42.21 °C). The hydrogels can be assembled into a strain sensor to monitor various human activities accurately at normal and low temperatures. This study proposes a strategy for designing hydrogels for supporting signal detection in cold environments.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RENEWABLE ENERGY
·
2024
·
IF 9
Xiangjun Guo,Yun Huang,Yunheng Tang,Ao Xia,Xianqing Zhu,Xun Zhu,Qiang Liao
Mixed bacterial syngas fermentation is a promising carbon-neutral method for producing acetic acid and ethanol. However, the bacterial community and metabolic pathways were different in the growth and ethanol production stages, whereas the activity of the bacterial community greatly depended on the pH of the culture. In this study, the effect of pH on syngas fermentation performance and microbial community composition under continuous gas supply was investigated. The mixed bacteria grew fastest at a pH of 7, while a constant pH of 6 led to the highest ethanol concentration of 5.15 g L −1 , which was 160 % higher than that at pH 7. Based on this, a two-step pH regulation method was proposed in this study to improve the ethanol production. Initially, the culture pH was maintained at 7 to create optimal growth conditions. Once bacterial density stabilized, it was then changed to a pH 6 to promote ethanol production. By doing this, compared with the control group, the maximum ethanol concentration (5.85 g L −1 ) increased by 148.9 %, the ethanol production rate (2.41 g L −1 d −1 ) increased by more than 8 times. This two-step pH regulation method ensured a high bacterial density and effectively stimulated ethanol production during syngas fermentation.
研究领域:
微生物学
·
生物技术
✓ 已验证
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Dongdong Ge,Haiping Yuan,Jiamu Xiao,Nanwen Zhu
Tannic acid (TA), a phenolic compound , may be considered as a sludge conditioning agent on account of its ability to precipitate protein. In this study, the effectiveness of TA conditioning on enhancing waste activated sludge (WAS) dewatering was investigated at various pH values. The results indicated that with the conditioning of 0.15 mmol TA per gram of total solid (TS), the WAS dewaterability was affected distinctly by the pH regulation. The reductions of 86.8% capillary suction time (CST), 96.3% specific resistance of filtration (SRF), and 23.6% water content (Wc) of dewatered sludge cake were achieved at an optimal pH value of 4.0. Meanwhile, obvious alterations were observed in some aspects like supernatant viscosity, zeta potential , particle size and extracellular polymeric substances (EPS) polymers. Correlation analysis indicated that the proteins in slime EPS and loosely bound EPS dominantly governed sludge dewaterability. Fluorescence quenching analysis indicated that in the range of acidity, the increase of pH value afforded more binding sites of sludge EPS for TA. However, the removal of EPS protein depended on the combined effect of TA conditioning and pH regulation. The findings provided a novel approach and explanation of WAS dewaterability enhancement using organic additive conditioning and pH regulation.
研究领域:
环境科学
·
污水处理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DIAMOND AND RELATED MATERIALS
·
2024
·
IF 4.3
Zhengpei Nie,Wenyu Zhong,Jiaman Liang,Qunwei Shu,Kejun Tan
Biomass carbon dots (CDs) typically suffer from red fluorescence quenching in water, limiting their practical applications. This study introduces a simple pH regulation strategy to enhance water-solubility by converting lipophilic surface groups to hydrophilic ones. Using mugwort as a precursor, novel triple-emission biomass-based CDs (T-CDs) with good water-solubility were successfully synthesized. Comprehensive structural and spectral characterizations were performed to elucidate the properties of the T-CDs and the origins of their luminescence, providing valuable insights for the design of future CDs. The T-CDs were further modified with hexadecyl trimethyl ammonium bromide (CTAB), producing modified triple-emission CDs (mT-CDs) with enhanced photostability. These mT-CDs were effectively employed as fluorescent probes for selective dopamine (DA) sensing, achieving a linear range of 0.8 to 300 μM and a limit of detection (LOD) of 0.17 μM, demonstrating their potential in biochemical applications. To explore additional applications, mT-CDs were incorporated into polyvinyl alcohol (PVA) to create thin films. These films exhibit pink fluorescence under 254 nm UV light and phosphorescence after the UV source is removed, making them suitable for information encryption and anti-counterfeiting purposes.
研究领域:
材料科学
·
生物传感
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2021
·
IF 3.764
Jianchuan Liu,Yunbo Zhang,Yao Miao,Ying Yang,Ping Li
The mechanism of lithium ion-sieve adsorbing Li+ ions from brines is based on the Li+/H+ ion exchange, where Li+ ions in brines are adsorbed on ion-sieves to displace H+ ions, and the displaced H+ ions are released in brines in turn. If the released H+ ions are accumulated in brines, then brines become acidic, not conducive to Li+ ions adsorption on ion-sieves. Therefore, it is important to regulate the pH value in brines to be more than 7 during the lithium recovery from brines using ion-sieves. Instead of the addition of the traditional ammonia buffer solution, the alkaline anion exchange resins are used to regulate the pH value of brines in this work since the addition of ammonia buffer solution in brines would cause the secondary pollution in Salt Lake with a high ammonia nitrogen emission value. The alkaline anion exchange resins contain the amine functional groups to neutralize the released H + ions in brines that enhance the adsorption performance of lithium ion-sieves. Like ammonia buffer, the amine functional groups of alkaline resins without more free OH– ions in brine will avoid the precipitation of high content of magnesium in brines. Here, seven kinds of alkaline anion exchange resins are used to neutralize the released H+ ions during lithium recovery from brines using homemade granular titanium-type lithium ion-sieves (PVB-HTO ion-sieves). The process intensification for lithium recovery from brine by lithium ion-sieves with the addition of alkaline anion exchange resins is evaluated based on experimental results, and a green lithium recovery technology from brine with a high Mg/Li ratio will be developed.
研究领域:
化学工程
·
环境科学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Dian Dai,Mingxiang Qv,Dongyang Liu,Chunming Tang,Wei Wang,Qirui Wu,Zhihong Yin,Liandong Zhu
Magnetic harvesting of microalgae depends on the interface interactions between microalgal cells and flocculants, and thus it is important to find a reasonable evaluation method understand these interface interactions. In this effort, we discussed the formation mechanism and pH regulation of microalgae magnetic harvesting via magnetic chitosan (Fe 3 O 4 @SiO 2 -CS) synthesized by a micro-emulsion method. The flocculation performances and mechanisms during the harvesting of microalgae by the novel flocculant were evaluated. The synthesis process and structure were determined by XRD, FT-IR, SEM and XPS, and the results indicated that Fe 3 O 4 @SiO 2 -CS was successfully prepared. The collection efficiency of 96.52 % was achieved for Chlorella vulgaris within 3 min using Fe 3 O 4 @SiO 2 -CS with the Fe 3 O 4 @SiO 2 and CS mass ratio of 1:3 under pH 1.0. Moreover, this study introduced the two-dimension fractal dimension and extended Derjaguin-Landau-Verwey-Overbeek (E-DLVO) model to evaluate the harvesting performances and mechanisms of Fe 3 O 4 @SiO 2 -CS. Experimental results showed that a strong electrostatic interaction was formed under acidic conditions between Fe 3 O 4 @SiO 2 -CS and microalgae cells with D 2 = 1.82 (fractal dimension value). The hydrophobic tryptophan-like amino acid was observed in the slime-extracellular polymeric substances (S-EPS) secreted by microalgae. S-EPS was found to be a significant substance to promote harvesting performance when the system was neutral or alkaline. This work provides deeper insights into microalgae harvesting by Fe 3 O 4 @SiO 2 -CS as well as reasonable mechanisms to illustrate the interaction of Fe 3 O 4 @SiO 2 -CS and microalgae under different pH values.
研究领域:
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）