INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Yan Li,Jiangtao Liu,Kehu Zhang,Lei Lei,Zhongli Lei
A hybrid polymer–MOFs architecture UiO-66-NH2@PMAA was synthesized by tethering polymethacrylic acid (PMAA) onto the surface of the metal–organic frameworks UiO-66-NH2, and it was further employed for pectinase immobilization by electrostatic interactions. The hybrid architecture was prepared through a combination of atom transfer radical polymerization (ATRP), click chemistry and postsynthetic modification (PSM). The optimal immobilization parameters were at 25 °C and pH 4.0 with 10 U/mL of pectinase for 2 h, under which pectinase showed the highest enzymatic activity (1.215 U/mg) and protein loading (448.5 mg/g), respectively. Compared with free pectinase, the immobilized was found to exhibit higher pH, thermo, and storage stability. Additionally, the immobilized pectinase had remarkable reusability, the residual activity reached as high as 81% after 8 cycles continuously. Meanwhile, the desorption of immobilized pectinase can be achieved by changing pH values of the medium. Obviously, the UiO-66-NH2@PMAA with attractive properties is highly promising for enzyme immobilization.
研究领域:
生物化学
·
材料科学
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DOI
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2018
·Vol. 43
·期 3
·e12741
·
IF 1.552
Si-Qi Yang,Xiao-Yan Dai,Xiao-Yi Wei,Qing Zhu,Tao Zhou
Co-immobilization of pectinase and glucoamylase onto sodium alginate/graphene oxide beads was achieved by N , N′ -dicyclohexylcarbodiimide/ N -hydroxysuccinimide as activating agent. The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively. Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts. Reusability studies indicated that both enzymes in I-PG retained over 60% of initial activity after six times of reuse. Conditions for the hydrolysis of the pumpkin–hawthorn compound juice by I-PG were optimized using orthogonal experiments. After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably. Therefore, the use of I-PG provided an effective and feasible method for improving quality of the pumpkin–hawthorn juice. Practical applications In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed. The co-immobilized pectinase/glucoamylase developed in this study could be applied in the clarification of juice rich in pectin and starch.
研究领域:
食品科学与工程
·
酶工程
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产品关联
DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2013
·Vol. 68
·期 6
·732-738
·
IF 0.879
Liu Yang,Li Qiang,Feng Yuan-Yuan,Ji Geng-Sheng,Li Tian-Cheng,Tu Jie,Gu Xu-Ding
Recent progress in nanotechnology has prompted research interest in immobilised enzymes on graphene oxide (GO) nanosheets for their large specific surface area and abundant functional groups. In the present work, acid pectinase was immobilised on the GO via the cross-linking of amino groups on pectinase and functional groups (e.g. carboxyl group) on the GO surface. Acid pectinase was effectively immobilised on the support and high loading densities were obtained (2400 mg per g of support). In addition, the immobilised enzyme achieved a better catalytic efficiency ( K
cat / K
m ) than its free counterpart; 3.7 mg −1 min −1 mL for immobilised pectinase, 3.5 mg −1 min −1 mL for free pectinase. Under acidic conditions, pectinase immobilised on GO will be agglomerated, but the addition of surfactant PEG 6000 could solve the problem and afford higher catalytic activity and catalytic efficiency.
研究领域:
生物化学
·
纳米技术
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DOI
高影响因子
JOURNAL OF FOOD ENGINEERING
·
2017
·
IF 3.099
Fusheng Zhang,Meiling Tian,Muying Du,Ting Fang
Pectinase is commercially important in the fruit processing and fiber degumming industries. Emerging technologies that improve its stability and activity could have potential in improving its commercial value further. The effects of pulsed electric field (PEF) treatment on the enzymatic activity, kinetics and conformational structure of pectinase were evaluated. Maximum activity was obtained at 12 kV/cm and a flow velocity of 80 mL/min, and was 21.89 ± 1.67% higher than the untreated enzyme. This enhancing effect could be maintained for 19 h when the treated enzyme was stored at 4 °C. PEF treatment increased the enzyme kinetic constants V max , k cat , t 1/2 , ΔG , and decreased K m , k , Ea , ΔH , and ΔS , indicating improved affinity between enzyme and substrate and enhanced catalytic efficiency. PEF treatment did not alter the polypeptide composition of the pectinase molecules, but induced the transfer of the tryptophan micro-environment from the polar surface to the hydrophobic interior, with a 10.28% increase in α-helices and a 17.80% reduction in random coils.
研究领域:
食品科学
·
生物技术
·
酶学
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产品关联
DOI
高影响因子
FIBERS AND POLYMERS
·
2024
·Vol. 25
·期 7
·2669-2682
·
IF 2.2
Luo Laipeng,Ding Chenkun,Zhu Qinting,Liu Lei,Wang Ping,Wang Qiang,Yu Yuanyuan
Untreated natural cotton fabrics are hydrophobic due to the high content of pectin and waxes, and the fabrics need to improve the wettability by scouring for the demands of the subsequent processing such as dyeing and finishing. In this study, a new scouring method for cotton fabrics was developed by pectin hydrolase combined with Fenton system (Fe 2+ /H 2 O 2 ). The mechanism of pectin degradation by pectinase combined with Fe 2+ /H 2 O 2 was investigated. The results showed that α-1,4-glycosidic bonds of water-soluble pectin could be degraded more efficiently by pectinase hydrolysis and Fe 2+ /H 2 O 2 oxidation. The fabrics scoured with pectinase → Fe 2+ /H 2 O 2 had less wetting time (4.25 s) and higher vertical wicking height (9.80 cm) compared to the fabrics treated with pectinase alone (> 120 s, 5.15 cm). The results of bleaching and dyeing experiments indicated that the whiteness and dyeing properties of the fabrics were not negatively affected by pectinase → Fe 2+ /H 2 O 2 treatment, and the strength loss of the fabrics (11.79%) was lower than that of the fabrics with conventional NaOH treatment (20.16%). The results of the experiments in the pilot-scale machine showed that various performance indicators of the fabrics scoured by pectinase → Fe 2+ /H 2 O 2 reached the level of the conventional NaOH treatment, which demonstrated the possibility for practical application of pectinase → Fe 2+ /H 2 O 2 scouring. The cotton fabric scouring using pectinase → Fe 2+ /H 2 O 2 is an effective and feasible new method with advantages such as environmental friendliness, mild treatment conditions, and good treatment effect.
研究领域:
纺织科学
·
生物化学
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DOI
高影响因子
CELLULOSE
·
2021
·Vol. 28
·期 13
·8375-8386
·
IF 5.044
Fan Fangwei,Zhu Mengting,Fang Kaiyang,Xie Jinpeng,Deng Zhongmin,Wang Xianfeng,Zhang Zhen,Cao Xinwang
This paper reports an improved traditional fiber degumming method, where sisal fibers were treated by alkali oxygen and pectinase, respectively, after the solute alkali pretreatment. To explore the influence of various factors on its degumming, efficiency of degumming through single factor and orthogonal experiments was aasessed. The results showed that pectinase/alkali-oxygen method after the first alkali treatment had a good effect on the degumming of sisal fiber, and most of the non-cellulose components such as hemicellulose, lignin and pectin had been removed. After pectinase treatment, the cellulose content and crystallinity were 71.87% and 66.29%, respectively. After alkaline oxygen treatment, the cellulose content was 77.16%, and the crystallinity was 69.09%. In terms of degumming rate, alkali oxygen treatment worked better than pectinase treatment, the degumming rate of pectinase method was about 10%, while that of alkali-oxygen method was more than 20%. In other hand, the pectinase method was much milder and had less damage to fibers. It would provide some references for the future application and development of sisal fiber.
研究领域:
生物化学
·
材料科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Gang Fang,Honggao Chen,Yunpeng Zhang,Anqing Chen
Fe 3 O 4 magnetic nanometer particles (MNPS) with a diameter of 30–40 nm and coated by ammoniated silicon dioxides were developed by sol–gel method. The X-ray diffraction (XRD) pattern indicates that the Fe 3 O 4 is wrapped in the amorphous SiO 2 , and the Fourier-transform infrared spectroscopy (FT-IR) analysis confirmed the NH 2 group had been successfully introduced onto the surface of Fe 3 O 4 @SiO 2 nanoparticles. The main factors related to the immobilization process of pectinase onto the magnetic support were optimized by multi-factor orthogonal experiments, and the recommended combination is that 3% glutaraldehyde (coupling agent), pH = 8.0, 50 °C, and cross-linking time 8 h. Scanning electron microscope (SEM) and FT-IR analyses confirmed that the pectinase had been immobilized onto the surface of the amino modified nanoparticles. The storage stability and reusability of the pectinase were enhanced by the immobilization that 64.4% of the total starting activity was retrieved after 7 recycling batches and only about 21.1% of the total activity was lost after 30-day storage. For the application of the immobilized pectinase, the optimal pH and temperature were 8.5 and 55 °C, respectively.
研究领域:
生物化学
·
纳米生物学
·
酶工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2026
·
IF 3.5
Jiangtao Liu,Xinyue Liu,Lei Lei,Hui Guo,Ruyi Jin,Zhi Li,Hao Yan
A temperature/light dual-stimuli-responsive polymeric carrier PPEGMA-b-PNIPAM-hv-PMAA-b-PS was designed and synthesized through host-guest mutual recognition, and further applied for pectinase immobilization. Both the host molecule PPEGMA-b-PNIPAM-β-CD and the guest molecule PS-b-PMAA-Azo were prepared via ATRP, hydrolysis, and click chemistry, respectively. The characterization of polymeric structure and morphology was carried out by 1H NMR, FTIR, and GPC. This polymer could self-assemble into spherical micelles in aqueous media, the critical micelle concentration (CMC) and the micellar morphology were also examined by UV–Vis, TEM, DLS, fluorescence spectra, etc. The enzyme immobilization property of the enzyme carrier was investigated too. It was revealed when pH was 4.0, immobilization concentration was 8 U/mL, temperature was 25 °C, and immobilization time was 60 min, both the immobilized amount and the relative activity could reach the maximum. By comparison with free pectinase, immobilized pectinase had a better tolerance to the variation of temperature and pH, and enhanced storage stability and reusability. Moreover, the relative activity of immobilized pectinase could be easily regulated by adjusting the temperature or UV irradiation. So, we can predict this immobilization material has a great application potential in industrial catalysis.
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2014
·
IF 5.039
Jingbo Li,Pengfei Zhou,Hongmei Liu,Chunjiang Xiong,Jianghai Lin,Wenjuan Xiao,Yingxue Gong,Zehuan Liu
Sugarcane bagasse (SCB) resulting from different pretreatments was hydrolyzed by enzyme cocktails based on replacement of cellulase (Celluclast 1.5 L:Novozym 188 = 1 FPU:4 pNPGU) by xylanase or pectinase at different proportions. Lignin content of NaOH pretreated SCB and hemicellulose content of H 2 SO 4 pretreated SCB were the lowest. NaOH pretreatment showed the best for monosaccharide production among the four pretreatments. Synergism was apparently observed between cellulase and xylanase for monosaccharide production from steam exploded SCB (SESB), NaOH, and H 2 O 2 pretreated SCB. No synergism was observed between cellulase and pectinase for producing glucose. Additionally, no synergism was present when H 2 SO 4 pretreated SCB was used. Replacement of 20% of the cellulase by xylanase enhanced the glucose yield by 6.6%, 8.8%, and 9.5% from SESB, NaOH, and H 2 O 2 pretreated SCB, respectively. Degree of synergism between cellulase and xylanase had positive relationship with xylan content and was affected by hydrolysis time.
研究领域:
生物化学
·
可再生能源
·
酶工程
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Hu Xu,Gen Li,Cheng Wan,Xiao-Yi Wei,Tao Zhou
In order to improve the extraction yield of lotus leaf polysaccharide (LLP) and offer scientific foundation for utilization of LLPs in the food and pharmaceutical industries, pectinase-assisted extraction method was employed for the preparation of LLP. The chemical composition, physicochemical properties (e.g. rheological and thermal properties etc.), antioxidant and anti-inflammatory effects of LLP and its two purified fractions (LLP1 and LLP3) obtained using DEAE-52 and subsequent Sephadex G-100 column chromatography were also investigated. Antioxidant assays indicated that the polysaccharides had remarkable DPPH• and ABTS +• scavenging activities. In H 2 O 2 -induced RAW264.7 cells, LLPs showed marked protective effect on oxidative stress, reducing the MDA and ROS contents and increasing the CAT and SOD activities. LLPs were also found to effectively modulate the expressions of CAT, SOD1, Nrf-2, HO-1, NQO1 and Keap1 mRNA. Thus, the potent antioxidant effects of LLPs were achieved possibly by regulating the Keap1/Nrf-2/HO-1 signaling pathways. In lipopolysaccharide-treated RAW264.7 macrophages, LLPs reduced phagocytic ability, decreased release of NO, TNF-α, IL-1β and IL-6, and increased release of IL-10 and TGF-β1, indicating their potent anti-inflammatory effect. LLPs downregulated expressions of iNOS, IL-1β, IL-6, TNF-α, COX-2, and TLR-4 mRNA. Therefore, modulation of NF-κB signaling pathway is possibly the important anti-inflammatory mechanism of LLPs.
研究领域:
食品科学
·
药理学
·
生物化学
·
分子生物学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Xiao-Yan Dai,Li-Min Kong,Xiao-Ling Wang,Qing Zhu,Kai Chen,Tao Zhou
Pectinase was immobilized onto sodium alginate/graphene oxide beads via amide bonds by using N , N ′-dicyclohexylcarbodiimide/ N -hydroxysuccinimide as the activating agent. The immobilized pectinase was characterized by Fourier transform infrared spectra and scanning electron microscopy analyses. Immobilization conditions were optimized by Box–Behnken design and the response surface method. The activity of the immobilized pectinase prepared under optimal conditions reached 1236.86 ± 40.21 U/g, with an enzyme activity recovery of 83.5%. The optimal pH of free pectinase was 4.5, while that of immobilized pectinase was shifted to 4.0. The optimal temperature of immobilized pectinase was increased to 60 °C, which was 10 °C higher than that of free form. Furthermore, the immobilized pectinase possessed a superior thermal stability and storage stability to those of free pectinase. Reusability studies indicated that the immobilized pectinase retained 73% of initial activity after six times cycles. Due to these good properties, such immobilized pectinase may find application in food industry.
研究领域:
生物化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
Plasmonics
·
2016
·Vol. 12
·期 3
·717-727
·
IF 2.146
Zhang Yan,Jiang Jingjing,Li Min,Gao Pengfei,Zhang Guomei,Shi Lihong,Dong Chuan,Shuang Shaomin
A highly selective and ultra-sensitive colorimetric assay has been developed for detecting the magnesium ion (Mg 2+ ) based on pectinase (PE)-protected Au nanoparticles (PE-AuNPs). This is the first study reporting the use of pectinase for green synthesizing gold nanoparticles. Aggregation of PE-AuNPs was induced immediately following the addition of Mg 2+ ion under Tris-HCl buffer at pH 4.0, yielding a color change from red to blue, and the characteristic surface plasmon resonance (SPR) peak of PE-AuNPs was red-shifted to 665 nm. The effects of parameters such as pH, the amount of PE-AuNPs, and incubation time on the sensitivity of colorimetric assay were investigated in detail. The Mg 2+ -induced aggregation of PE-AuNPs could be monitored by both the naked eye and UV–vis spectrophotometry. The lowest detection concentration with the naked eye is 0.52 μM. A linear relationship between the absorbance ratio ( A
665 / A
523 ) and Mg 2+ concentration was observed in two ranges of Mg 2+ concentration by UV–vis spectrophotometry, including 5.34 × 10 −7 μM to 51.4 μM and 5.12 × 10 2 μM to 3.19 × 10 3 μM. The Mg 2+ detection limit was determined to be 4.0 × 10 −9 μM with UV–vis spectrophotometer. The proposed colorimetric assay possesses a highly selective response for Mg 2+ over other metal ions. This method has been successfully applied to determine the Mg 2+ ion in some water samples.
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DOI
高影响因子
危险品化学品经营许可证（带存储）