AIE细菌红色探针是一种基于聚集诱导发光效应的新型细菌探针，选择性地定位在三种病原体的不同部位，在紫外光激发下，可用肉眼观察出三种可辨别的发射颜色（革兰氏阴性菌呈淡粉红色，革兰氏阳性菌呈橙红色，真菌呈亮黄色）。也能基于其独特的荧光特性，利用酶标仪，实现不同细菌的区分，同时，本产品可实现对活细菌的长时间追踪成像。
预期用途
用于活细菌及真菌的染色。
检验原理
本产品是一种基于聚集诱导发光效应的新型细菌探针，选择性地定位在三种病原体的不同部位，在紫外光激发 下，可用肉眼观察出三种可辨别的发射颜色（革兰氏阴性菌呈淡粉红色，革兰氏阳性菌呈橙红色，真菌呈亮黄色）。 也能基于其独特的荧光特性，利用酶标仪，实现不同细菌的区分，同时，本产品可实现对活细菌的长时间追踪成像。
产品效果
AIE细菌红色探针染色效果图，左：白假丝酵母菌，右：金黄色葡萄球菌
样本要求
适用于培养后的活细菌或真菌样本。
检验方法
使用本产品前请仔细阅读本说明书。
1、染料储备液准备：短暂离心后，将染料储备液适当分装后避光保存于-20℃或更低温度；
2、细菌准备：培养过夜的细菌，离心、用PBS洗涤2-3次、浓缩至2 mL菌液，建议细菌浓度OD600值为0.2-1.5；
3、细菌染色：取1 µL储备液加入到浓缩菌液中，使探针的最终浓度在 5-10 μM，对于特定细菌，可以适 当增加或者减少探针的浓度已达到最佳成像效果；
4、染色成像：将菌液常温放置10 min左右，取2-3 µL滴于载玻片上，盖上盖玻片，用共聚焦荧光显微镜 观察。激发波长设为450 nm，发射波长设为570-630 nm。
注意事项
1. 使用本品时应佩戴一次性手套，避免和皮肤、眼睛接触，一旦接触，立即用大量清水冲洗，如果误服或 者溅入眼睛，请及时就医。
2. 于-20℃保存，避免反复冻融；
3. 所有样本、生物试剂及实验物品都应被视为有潜在传染性，须根据当地法规按照医疗废物处理。
4. 使用本产品过程中如有问题或建议，请与厂家联系。
AIE Bacteria Red Probe is a new type of bacterial probe based on the aggregation-induced emission effect. It selectively localizes to different parts of three pathogens. Under ultraviolet light excitation, three distinguishable emission colors can be observed with the naked eye (Gram-negative bacteria appear pale pink, Gram-positive bacteria appear orange-red, and fungi appear bright yellow). Based on its unique fluorescent properties, it can also distinguish different bacteria using a microplate reader. Additionally, this product enables long-term tracking and imaging of live bacteria.
Intended Use
For staining live bacteria and fungi.
Test Principle
This product is a new type of bacterial probe based on the aggregation-induced emission effect. It selectively localizes to different parts of three pathogens. Under ultraviolet light excitation, three distinguishable emission colors can be observed with the naked eye (Gram-negative bacteria appear pale pink, Gram-positive bacteria appear orange-red, and fungi appear bright yellow). Based on its unique fluorescent properties, it can also distinguish different bacteria using a microplate reader. Moreover, this product allows long-term tracking and imaging of live bacteria.
Product Effect
Imaging effect of AIE bacterial red probe staining. Left: Candida albicans; Right: Staphylococcus aureus
Sample Requirements
Suitable for cultured live bacteria or fungal samples.
Test Method
Please read this instruction manual carefully before using this product.
1.Preparation of dye stock solution: After brief centrifugation, appropriately aliquot the dye stock solution and store it protected from light at -20°C or lower;
2.Bacterial preparation: Bacteria cultured overnight are centrifuged, washed 2-3 times with PBS, and concentrated to 2 mL of bacterial solution. It is recommended that the bacterial concentration has an OD600 value of 0.2-1.5;
3.Bacterial staining: Add 1 µL of stock solution to the concentrated bacterial solution to make the final concentration of the probe 5-10 μM. For specific bacteria, the concentration of the probe can be appropriately increased or decreased to achieve the best imaging effect;
4.Staining and imaging: Place the bacterial solution at room temperature for about 10 minutes, take 2-3 µL and drop it on a glass slide, cover with a coverslip, and observe with a confocal fluorescence microscope. The excitation wavelength is set to 450 nm, and the emission wavelength is set to 570-630 nm.
Precautions
1.Wear disposable gloves when using this product to avoid contact with skin and eyes. In case of contact, rinse immediately with plenty of water. If swallowed or splashed into eyes, seek medical attention promptly.
2.Store at -20°C and avoid repeated freezing and thawing;
3.All samples, biological reagents, and experimental items should be regarded as potentially infectious and must be disposed of as medical waste in accordance with local regulations.
4.If you have any questions or suggestions during the use of this product, please contact the manufacturer.
ZJ25F1028440
危险品化学品经营许可证（带存储）