计算溶液所需的质量、体积或浓度。
迪夫快速染色液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Diff-Quik 染色是在 Wright 染色基础上改良而来的一种快速染色方法，是细胞学检查中常用的染色方法之一，染液是采用世界卫生组织（WHO）推荐的快速染色方法而配制，与Wright Stain类似都是利用Romanowsky Stain技术原理改良而来的，染色结果与瑞氏染色液也极其相似，但迪夫快速染色所需的时间极短，一般90 s以内即可完成染色。
迪夫快速染色液含固定液，主要用于血细胞涂片、骨髓涂片、阴道分泌物涂片、脱落细胞涂片等染色，非常适合用于批量浸染，且背景清晰无沉渣。该染色液仅用于科研领域，不宜用于临床诊断或其他用途。
| D774859 | Component | 3×100 mL | 3×500 mL | Storage |
| D774859A | Diff-Quik Fixative | 100 mL | 500 mL | RT. Store in the dark |
| D774859B | Diff-Quik Ⅰ | 100 mL | 500 mL | RT. Store in the dark |
| D774859C | Diff-Quik Ⅱ | 100 mL | 500 mL | RT. Store in the dark |
使用说明
1. 常规方法制备血液涂片或骨髓涂片，自然干燥或酒精灯火焰干燥后，Diff-Quik Fixative固定20~30 s。
注意：根据待检样本的不同，采用的固定策略也不同，具体请参照注意事项3~5。
2. Diff-Quik Ⅰ染色5~10 s。
3. Diff-Quik Ⅱ染色10~20 s。
4. 水洗后立即趁湿在显微镜下观察。
5. 将观察后认为有价值的片子带回实验室，用二甲苯透明，封固、保存。
注意事项
1. 血液涂片或骨髓涂片应厚薄均匀，以免影响染色效果。
2. 血细胞涂片染色要求新鲜全血或 EDTA 抗凝血。血膜未干透，细胞有可能尚未牢固附在玻片上，在染色过程中容易脱落，因此血膜必需充分干燥。
3. 骨髓涂片染色要求制备好的涂片在空气中快速摇动或扇干，以防止细胞皱缩变形或因空气潮湿而溶血，不能用高温或火烤来干燥涂片。
4. 阴道分泌物涂片染色要求新鲜标本涂片后，尽快以火焰或酒精固定，以免细胞变形。
5. 脱落细胞涂片固定可采用自然干燥或固定液固定。
6. 涂片染色中请勿先去除染液或直接对涂片用力冲洗。不能先倒掉染液，以免染料沉着于涂片上。
7. 染色液可重复使用，但不能多次重复，若有沉淀物应过滤后使用。
8. 染色过深可用甲醇或酒精适当脱色，最好不复染。
9. 如果染色过深或过浅，应调整染色时间或工作液浓度。
10. pH值对染色有一定影响，载玻片应清洁、无酸碱污染，以免影响染色效果。
11. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
染色结果
| 细胞核、白细胞 | 深蓝色 |
| 基质、淋巴细胞 | 紫色 |
| 细胞质、红细胞 | 粉红色 |
Diff-Quik Staining is a rapid staining method modified from Wright Staining and is one of the commonly used staining techniques in cytological examinations. The staining solution is formulated based on the rapid staining method recommended by the World Health Organization (WHO). Similar to Wright Stain, it is derived from the Romanowsky Stain technique. The staining results are also very similar to those of Wright's stain, but Diff-Quik staining requires an extremely short time—typically completed within 90 seconds.
The Diff-Quik Staining Solution contains a fixative and is primarily used for staining blood cell smears, bone marrow smears, vaginal secretion smears, exfoliative cell smears, etc. It is particularly suitable for batch immersion staining and provides a clear background without precipitate. This staining solution is intended for research use only and should not be used for clinical diagnosis or other purposes.
| D774859 | Component | 3×100 mL | 3×500 mL | Storage |
| D774859A | Diff-Quik Fixative | 100 mL | 500 mL | RT. Store in the dark |
| D774859B | Diff-Quik Ⅰ | 100 mL | 500 mL | RT. Store in the dark |
| D774859C | Diff-Quik Ⅱ | 100 mL | 500 mL | RT. Store in the dark |
Instructions for Use
Prepare blood or bone marrow smears using standard methods. After air-drying or drying over an alcohol lamp flame, fix with Diff-Quik Fixative for 20–30 seconds.
Note: The fixation strategy varies depending on the sample. Please refer to Notes 3–5 for details.
Stain with Diff-Quik Solution Ⅰ for 5–10 seconds.
Stain with Diff-Quik Solution Ⅱ for 10–20 seconds.
Rinse with water and immediately examine under a microscope while wet.
For slides deemed valuable after observation, return them to the lab for clearing with xylene, followed by mounting and preservation.
Precautions
Blood or bone marrow smears should be of uniform thickness to avoid affecting staining quality.
For blood cell smears, use fresh whole blood or EDTA-anticoagulated blood. The blood film must be completely dry; otherwise, cells may detach during staining.
For bone marrow smears, air-dry prepared slides quickly by waving or fanning to prevent cell shrinkage, deformation, or hemolysis due to humidity. Do not dry using high heat or flame.
For vaginal secretion smears, fix fresh specimens promptly with flame or alcohol to prevent cell deformation.
For exfoliative cell smears, fixation can be achieved by air-drying or using a fixative solution.
During staining, do not pour off the stain first or rinse the slide forcefully, as this may cause dye precipitation on the smear.
Staining solutions can be reused but not excessively. Filter if precipitates form.
Over-stained slides can be decolorized with methanol or alcohol. Re-staining is not recommended.
Adjust staining time or working solution concentration if results are too dark or too light.
pH affects staining. Ensure slides are clean and free from acid/alkali contamination.
Wear a lab coat and disposable gloves for safety.
Staining Results
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规格
规格或纯度
BioReagent, 用于显微镜, 生物染色剂
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Diff-Quik Staining Solution
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0939174 |
分析证书 | D774859 |
| ZJ26F0838441 |
分析证书 | D774859 |
| ZJ25F0521900 |
分析证书 | D774859 |
| ZJ26F0232309 |
分析证书 | D774859 |
技术文档和文章
溶液计算器
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