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来自于微生物的肝素（Hep）/硫酸乙酰肝素（HS）降解酶（Hepases）属于多糖裂解酶，以典型的b-消除机制裂解N-乙酰葡萄糖胺（GlcNAc）和己糖醛酸（GlcUA/IdoUA）之间的a-1,4糖苷键，并在己糖醛酸的C4和C5位生成一个在232 nm有特异吸收的不饱和双键，该双键的引入有利于酶解产物的分析检测。HEPases在Hep/HS降解菌中被鉴定，如Flavobacterium heparinum, Bacteroides thetaiotaomicron，Bacteroides stercoris，Sphingomonas, Bacillus , Pseudomonas aeruginosa[等。根据底物选择性HEPases可以分为三大类：HEPase I选择性降解Hep及HS中的高硫酸化区；HEPase III则选择性降解HS及Hep中的低硫酸化区；而HEPase II则可以同时降解Hep和HS。以上这三类HEPases都属于内切酶，最近一个肝素外切酶家族（exoHEPases）被首次发现。HEPases作为重要的工具酶，被广泛应用于Hep/HS的结构和功能研究、低分子肝素的制、肝素类药物质量检测和一致性评价等。我们可以根据客户的需求，为客户提各种已知类型的HEPase，满足客户从分析检测到规模化生产等各种要求的HEPase酶制剂。
应用
来自肝素黄杆菌的肝素酶I和II混合物已被用于：消化绵羊玻璃体的硫酸乙酰肝素；人胚胎肾细胞；来自动脉组织的糖胺聚糖；P0 视网膜消化。
Hepases, derived from microorganisms, are polysaccharide lyases that cleave the a-1,4 glycosidic bond between N-acetylglucosamine (GlcNAc) and hexuronic acid (GlcUA/IdoUA) through a typical b-elimination mechanism. They generate an unsaturated double bond with specific absorption at 232 nm at the C4 and C5 positions of hexuronic acid, which facilitates the analysis and detection of enzymatic hydrolysis products. HEPases have been identified in Hep/HS degrading bacteria, such as Flavobacterium heparinum, Bacteroides thetaiotaomicron，Bacteroides stercoris，Sphingomonas, Bacillus , Pseudomonas aeruginosa[ Wait. HEPases can be divided into three categories based on substrate selectivity: HEPase I selectively degrades the high sulfation zone in Hep and HS; HEPase III selectively degrades the low sulfur acidification zone in HS and Hep; HEPase II can degrade both Hep and HS simultaneously. The above three types of HEPases belong to endonucleases, and recently a heparin exonuclease family (exoHEPases) has been discovered for the first time. HEPases, as an important tool enzyme, are widely used in the structural and functional research of Hep/HS, the production of low molecular weight heparin, quality testing and consistency evaluation of heparin drugs, etc. We can provide customers with various known types of HEPase enzyme preparations according to their needs, meeting their various requirements from analysis and detection to large-scale production.
Application
Heparinase I and III Blend from Flavobacterium heparinum has been used in:the digestion of heparan sulfate from ovine vitreous；human embryonic kidney cells；glycosaminoglycans from arterial tissues；P0 retinae digestion.
H2515400
危险品化学品经营许可证（带存储）