计算溶液所需的质量、体积或浓度。
BioReagent 级 提供 ≥80% 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
PHA由两种分子种类组成,即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素(PHA-E),和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素(PHA-L)。PHA-E不具有血型特异性,但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前. PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。
处理说明
为了复溶,将1.0 ml无菌Dulbecco磷酸盐缓冲液(DPBS)或组织培养基加入到5 mg凝集素中,并轻轻旋转。 溶液可能略显浑浊。使用前,可采用无菌缓冲液将复溶产品进一步稀释至所需工作浓度。应避免过滤,以防止任何产品损失。复溶后,将分装样品储存于-20℃。不建议长期储存产品和反复冻融。
产品应用
红细胞凝集实验和糖基化研究。
注:凝集活性是通过连续稀释1 mg/ml溶液来测定的。这种活性是在25℃下孵育1小时后,使悬浮的人白细胞(每毫升含10^7个细胞)发生凝集的最低浓度。
PHA consists of two molecular species: erythroagglutinin (PHA-E), which exhibits low mitogenic activity and high hemagglutinating activity; and leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity but very low hemagglutinating activity. PHA-E shows no blood group specificity, yet its agglutinating activity can be inhibited by certain oligosaccharides. Prior to the separation and purification of erythroagglutinin and leucoagglutinin, PHA-P represents the protein form of PHA, while PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Reconstitution Instructions
For reconstitution, add 1.0 mL of sterile Dulbecco’s Phosphate-Buffered Saline (DPBS) or tissue culture medium to 5 mg of the lectin and swirl gently. The solution may appear slightly turbid. The reconstituted product can be further diluted to the required working concentration using sterile buffer prior to use. Filtration should be avoided to prevent any product loss. After reconstitution, aliquot samples and store at −20 °C. Long-term storage and repeated freeze-thaw cycles of the product are not recommended.
Applications
Hemagglutination assay and glycosylation research.
Note:Agglutinating activity is determined by serial dilution of a 1 mg/mL solution. The activity is defined as the lowest concentration capable of inducing agglutination of suspended human leukocytes (10⁷ cells per mL) following incubation at 25 °C for 1 hour.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | L755097 | |
| 分析证书 | L755097 | |
| 分析证书 | L755097 | |
| 分析证书 | L755097 |