BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光,禁止冷冻。低温运输,禁止冷冻 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
储存缓冲液：Lyophilized from 5.0mg/mL bulk protein in a 0.22μm filtered solution of 0.1M PB, 0.15M NaCl, 10mM EDTA, 3% trehalose, PH=7.2
复溶：Reconstitute in ultrapure water
阿拉丁生产的LumiDye™ SMCC Activated APC (SMCC-APC)，即SMCC活化别藻蓝蛋白，是由4-(N-马来酰亚胺基甲基)环已烷-1-羟酸琥珀酰亚胺酯(Succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate, SMCC)修饰别藻蓝蛋白(APC)形成，可与含巯基的分子如蛋白等偶联，偶联物常用于荧光显微检测、荧光免疫测定、双色或多色荧光分析、癌细胞表面抗原检测、流式细胞荧光测定等抗体荧光标记以及活体成像等诊断及生物工程技术。
APC是从螺旋藻(一种蓝绿藻)中分离出来的藻胆蛋白，分子量约为104kDa，具有极高的量子产率和吸收率，可以呈现出强烈且稳定的荧光，易于其它分子交联，非特异性吸附少，灵敏度比常规有机荧光团高100倍以上，因而在生物检测中被广泛用作荧光标签。APC最大激发波长(Ex)为650nm，荧光最大发射波长(Em)为660nm。
本产品为APC与SMCC的连接产物，经纯化并去除过量的SMCC，溶于含3%海藻糖的PBS-EDTA中，SMCC-APC最终以冻干粉的形式提供，长期储存也不损失巯基反应性，保证了SMCC-APC与其它含巯基分子如蛋白质等偶联的最佳性质。
使用方法
SMCC-APC具有巯基反应性，可以针对抗体等生物大分子进行偶联，这些分子具有半胱氨酸，可以通过还原剂处理以产生游离的巯基基团。当蛋白等生物大分子具有可用的伯胺基团时，也可以通过化学反应添加所需的巯基基团。
本产品最佳使用浓度为5mg/mL，加入200 uL 纯水，摇晃瓶体使染料完全溶解后可进行标记。注：本产品需现用现配。
保存条件
4ºC避光保存，12个月内有效，切勿-20ºC或更低温度保存。
注意事项
1. 避免在反应过程中使用含有伯胺和巯基的缓冲液，以减少不必要的竞争反应；
2. 荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光的淬灭；
3. 本产品的偶联产物，须避光保存，请勿冷冻；
4. 操作过程中，务必穿戴实验服、一次性手套及防护装备；
5. 所有产品仅限于研究用途。
LumiDye™ SMCC Activated APC (SMCC-APC) is formed by modifying allophycocyanin (APC) with Succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC). It contains a maleimide group that reacts with thiol groups of target molecules, such as proteins. This product is commonly used in fluorescence microscopy, fluorescence immunoassays, dual or multicolor fluorescence analysis, cancer cell surface antigen detection, flow cytometry fluorescence assays, antibody fluorescence labeling, as well as diagnostics and biotechnology applications such as live cell imaging.
APC (allophycocyanin) is a phycobiliprotein isolated from spirulina (a type of blue-green algae). It has a molecular weight of approximately 104kDa and exhibits a very high quantum yield and absorbance, resulting in strong and stable fluorescence. It is easily cross-linked with other molecules and has minimal non-specific adsorption. Its sensitivity is more than 100 times higher than conventional organic fluorophores, making it widely used as a fluorescent label in biological assays. The maximum excitation wavelength (Ex) of APC is 650nm, and the maximum emission wavelength (Em) is 660nm.
This product is a purified conjugate of APC and SMCC. After purification to remove excess SMCC, it is dissolved in PBS-EDTA with 3% trehalose and ultimately supplied as a lyophilized powder. The long-term storage of this product does not affect its thiol reactivity, ensuring the optimal properties for conjugation with other thiol-containing molecules such as proteins.
Instruction for use
This product is chemically activated for easy conjugation to biomacromolecules such as antibodies. These molecules contain cysteine residues. Disulfide bonds formed between cystein residues can be treated with a reducing agent, such as DTT and β-mercaptoethanol, to release free thiol groups. Thiol groups can also be added to primary amine groups of biomacromolecule, such as a protein, through chemical reactions.
The optimal concentration for this product is 5 mg/mL. Add 200 μL of ultrapure water, gently swirl the bottle to ensure complete dissolution of the dye, then proceed with labeling. Note: The SMCC-APC solution must be prepared fresh before each use.
Figure 1. Schematic diagram of the conjugation of LumiDye™ SMCC Activated APC (SMCC-APC) (M1491730） to proteins.
Storage
Store at 4℃ in the dark for up to 12 months. Do not store at -20℃ or lower temperatures.
Matters needing attention
1. Avoid using buffers containing primary amines and thiols during the reaction to minimize unnecessary competitive reactions.
2. Fluorescent dyes are prone to quenching. Please store and use them in the dark to slow down fluorescence quenching.
3. Products conjugated with the SMCC-APC should be stored at 4℃ in the dark. Do not freeze.
4. During operation, always wear a lab coat, disposable gloves, and protective equipment.
5. All products are for research use only.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0231996 |
分析证书 | M1491730 |
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