中性蛋白酶 AF 来源于溶组织梭菌

CAS: 9001-92-7 货号: N278967 EC号: 232-642-4
有货
级别和纯度: EnzymoPure™ ? EnzymoPure™ —— 阿拉丁的高质量酶解决方案系列。当酶纯度和明确活性决定检测或工艺性能时使用。 ≥ 0.50 U/mg
Accession #
P07519
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规格
库存
价格
数量
10U
N278967-10U
期货 Stock Image
¥2,999.90
50U
N278967-50U
期货 Stock Image
¥9,999.90
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为什么选择此级别

EnzymoPure™ 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 28 篇同行评审文献引用。

概述

Description

Neutral Protease AF is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This purified metalloprotease is suitable for the dissociation of tissue in clinical applications. For especially gentle cell isolation it is recommended to use Neutral Protease AF in combination with highly purified Collagenase AF-1 

 

Specifications

Plant-based fermentation process. Contains chromatographically purified neutral protease.
Vial contains not less than 100 DMC U of neutral protease.

Neutral protease activity (DMC) ≥ 0.50 U/mg.

Endotoxin: ≤ 100 EU/mg (Ph. Eur.)

Largely free of collagenolytic activity.

Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia
Endproduct is 0.22 µm filtered.

 

References and Definitions

Unit definition:Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).
Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)]. 


References

1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93

Description

Neutral Protease AF is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This purified metalloprotease is suitable for the dissociation of tissue in clinical applications. For especially gentle cell isolation it is recommended to use Neutral Protease AF in combination with highly purified Collagenase AF-1 

 

Specifications

Plant-based fermentation process. Contains chromatographically purified neutral protease.
Vial contains not less than 100 DMC U of neutral protease.

Neutral protease activity (DMC) ≥ 0.50 U/mg.

Endotoxin: ≤ 100 EU/mg (Ph. Eur.)

Largely free of collagenolytic activity.

Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia
Endproduct is 0.22 µm filtered.

 

References and Definitions

Unit definition:Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).
Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)]. 


References

1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93

规格

产品名称
中性蛋白酶 AF 来源于溶组织梭菌
英文别名
Neutral Protease
规格或纯度
EnzymoPure™, ≥ 0.50 U/mg
CAS编号和信息
9001-92-7
酶学委员会编号
3.4.24.33
分子类型
酶
产品线
生物产品
储存与运输
浓度
≥ 0.50 U/mg
储存条件
2-8°C储存
运输条件
低温运输
单位定义
1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).

技术文档

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
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此产品的引用文献
溶液计算器

常见问题

本产品应如何储存?
请于2–8 °C条件下保存。需冷藏保存以维持规定的有效期。
本产品如何运输?
本产品采用湿冰冷藏运输。收货后请立即拆包,并按上述储存条件保存。
本产品的 CAS 号、分子式和分子量是多少?
本产品的 CAS 号为 9001-92-7。
「EnzymoPure?」是什么?
「EnzymoPure?」是阿拉丁科学的高端产品线之一,专为多个工作流程中的高纯度应用而设计。与同类工作流程中的标准级别相比,「EnzymoPure?」产品通常具有更严格的规格、更严谨的 QC 以及更强的批间一致性。
如何获取批次专用COA?
每个订单都会随附分析证书(COA),您也可以从账户订单历史中下载。如需订购前的COA,请联系您的客户代表,或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。