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EnzymoPure™ 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 28 篇同行评审文献引用。
Description
Neutral Protease AF is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This purified metalloprotease is suitable for the dissociation of tissue in clinical applications. For especially gentle cell isolation it is recommended to use Neutral Protease AF in combination with highly purified Collagenase AF-1
Specifications
Plant-based fermentation process. Contains chromatographically purified neutral protease.
Vial contains not less than 100 DMC U of neutral protease.
Neutral protease activity (DMC) ≥ 0.50 U/mg.
Endotoxin: ≤ 100 EU/mg (Ph. Eur.)
Largely free of collagenolytic activity.
Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia
Endproduct is 0.22 µm filtered.
References and Definitions
Unit definition:Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).
Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)].
References
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93
Description
Neutral Protease AF is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This purified metalloprotease is suitable for the dissociation of tissue in clinical applications. For especially gentle cell isolation it is recommended to use Neutral Protease AF in combination with highly purified Collagenase AF-1
Specifications
Plant-based fermentation process. Contains chromatographically purified neutral protease.
Vial contains not less than 100 DMC U of neutral protease.
Neutral protease activity (DMC) ≥ 0.50 U/mg.
Endotoxin: ≤ 100 EU/mg (Ph. Eur.)
Largely free of collagenolytic activity.
Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia
Endproduct is 0.22 µm filtered.
References and Definitions
Unit definition:Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).
Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)].
References
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93