Phaseolus vulgaris lectin (PHA-E) is isolated from red kidney beans and purified by affinity chromatography and ion-exchange chromatography. It is a tetrameric protein with a molecular weight of 128 kDa. The lectin recognizes and binds specifically to terminal galactose, N-acetylglucosamine and mannose residues of complex glycans on mammalian glycoproteins. Because of its strong erythrocyte agglutinating activity, it is also known as Erythroagglutinin (PHA-E). PHA-E is not blood group specific. Less than 5 μg/ml agglutinates human erythrocytes of type O and agglutination can be inhibited by certain oligosaccharides. PHA-E lectin is supplied as a white lyophilized powder from a buffer containing from 50 mM NH4HCO3. The purity of the lectin in determined by SDS-PAGE, which generate one single band at 34 kDa. Erythrocyte agglutination studies;Model system of how proteins recognize carbohydrates● Ultrapure quality ● Sμgar specificity: complex oligosaccharides ● Strong erythroagglutinin activity ● Lyophilized powderRed kidney beans

Phaseolus vulgaris lectin (PHA-E) is isolated from red kidney beans and purified by affinity chromatography and ion-exchange chromatography. It is a tetrameric protein with a molecular weight of 128 kDa. The lectin recognizes and binds specifically to terminal galactose, N-acetylglucosamine and mannose residues of complex glycans on mammalian glycoproteins. Because of its strong erythrocyte agglutinating activity, it is also known as Erythroagglutinin (PHA-E). PHA-E is not blood group specific. Less than 5 μg/ml agglutinates human erythrocytes of type O and agglutination can be inhibited by certain oligosaccharides. PHA-E lectin is supplied as a white lyophilized powder from a buffer containing from 50 mM NH4HCO3. The purity of the lectin in determined by SDS-PAGE, which generate one single band at 34 kDa.
● Ultrapure quality
● Sugar specificity: complex oligosaccharides
● Strong erythroagglutinin activity
● Lyophilized powder
Erythrocyte agglutination studies;Model system of how proteins recognize carbohydrates

规格

别名

植物血球凝集素

英文别名

Lectin from Phaseolus vulgaris (red kidney bean) | Phaseolus vulgaris agglutinin | PHA

英文名称

Phaseolus vulgaris lectin E (PHA-E)

生化机理

PHA 由两种分子组成，一种是红细胞凝集素（PHA-E），它具有较低的有丝分裂活性和较高的红细胞凝集素活性；另一种是白细胞凝集素（PHA-L），它具有较高的有丝分裂活性和白细胞凝集素活性，但红细胞凝集素活性很低；PHA-E 没有血型特异性，但某些寡糖可抑制凝集。

储存条件

-20°C储存

运输条件

超低温运输

名称和识别符

分子类型

蛋白质

高级数据

化学和物理性质

敏感性

对湿度敏感

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