The experimental value of lectin-based detection lies in converting glycan recognition into signals that can be localized, compared, and validated. Histochemistry, ELISA, and Western blot correspond to three different levels of information output: spatial distribution analysis, solid-phase ...
The core of glycoprotein structural characterization does not lie in confirming a single chemical entity, but in resolving a multilayered information system jointly defined by the protein backbone, glycosylation sites, site occupancy, glycan composition, branching patterns, terminal ...