Recombinant Human GP5 Protein: FAQs & Research Guide
Recombinant Human GP5 Protein: FAQs & Research Guide
Recombinant Human GP5 Protein is a research-grade reagent representing the platelet surface glycoprotein V (GP5/GPV), a key component of the GPIb-IX-V receptor complex involved in platelet adhesion and von Willebrand factor-mediated hemostasis.
- Product Name
- Recombinant Human GP5 Protein
- SKU
- rp364822
- Protein Target
- Glycoprotein V (GP5/GPV), platelet surface receptor subunit
- Biological Role
- Component of the GPIb-IX-V complex; involved in platelet activation, adhesion, and thrombin signaling
- Research Applications
- Binding assays, functional studies, antibody production, ELISA, platelet biology research
- Storage Recommendation
- Avoid repeated freeze-thaw cycles; store at -20°C or -80°C as recommended in the product datasheet
- Reference Code
- absea#PQ-524
What Is Recombinant Human GP5 Protein?
Glycoprotein V (GP5, also known as GPV or CD42d) is a type I transmembrane glycoprotein expressed on the surface of platelets and megakaryocytes. It is a subunit of the GPIb-IX-V complex, a major platelet adhesion receptor that mediates the initial tethering of platelets to sites of vascular injury via von Willebrand factor (vWF) under high shear conditions.
Recombinant Human GP5 Protein (SKU: rp364822) is a recombinant form of this protein produced using molecular biology techniques, enabling consistent, animal-free supply of the target antigen for in vitro research applications. The recombinant protein allows researchers to study GP5 structure, receptor interactions, and platelet signaling pathways without relying on platelet-derived material.
Key Research Applications of Recombinant Human GP5 Protein
Recombinant Human GP5 Protein is suitable for a range of in vitro research applications relevant to platelet biology, hemostasis, and thrombosis research:
- Binding and interaction assays: Study the interactions between GP5 and other components of the GPIb-IX-V complex, or with potential ligands and inhibitors.
- Antibody development and validation: Use as an antigen for generating or validating antibodies targeting GP5 in immunoassays.
- ELISA and immunoassay development: Serve as a standard or capture antigen in sandwich ELISA formats.
- Functional and signaling studies: Investigate the role of GP5 in thrombin signaling and platelet activation pathways.
- Drug discovery screening: Screen small molecules or biologics that modulate the GPIb-IX-V complex function.
Researchers should consult the product datasheet for validated assay formats and recommended working concentrations.
Storage, Handling, and Stability Guidelines
Proper storage and handling of Recombinant Human GP5 Protein are essential to maintain protein integrity and biological activity throughout the reagent's shelf life:
- Temperature: Store at -20°C for short-term use or -80°C for long-term storage, as specified in the product datasheet.
- Freeze-thaw cycles: Avoid repeated freeze-thaw cycles, as these can lead to protein aggregation and loss of activity. Aliquot the protein upon first thaw to minimize repeated freeze-thaw events.
- Carrier proteins: If the formulation does not include a stabilizing carrier, consider adding BSA to prevent adsorption to tube surfaces at low concentrations.
- Working solutions: Prepare working dilutions fresh on the day of the experiment where possible.
Always refer to the specific product datasheet (absea#PQ-524) supplied with the product for formulation details, recommended diluents, and confirmed shelf-life information.
Quality, Purity, and Regulatory Considerations
Recombinant Human GP5 Protein from Aladdin Scientific is manufactured as a research-use-only (RUO) reagent. Key quality considerations include:
- Purity: Recombinant proteins in this category are typically assessed by SDS-PAGE and may also be characterized by Western blot or mass spectrometry. Specific purity data should be confirmed in the product datasheet (absea#PQ-524).
- Endotoxin levels: For functional cell-based assays, endotoxin content is a critical quality attribute. Consult the datasheet for LAL-tested endotoxin values.
- Biological activity: Activity may be confirmed by binding or functional assays as described in the datasheet.
- RUO designation: This product is intended for research use only and is not suitable for diagnostic or therapeutic applications in humans.
Customers requiring certificates of analysis (CoA) or safety data sheets (SDS) should contact Aladdin Scientific customer service or access these documents via the product page.
Ordering and Technical Support
Recombinant Human GP5 Protein is available from Aladdin Scientific under SKU rp364822. To place an order or request a quote:
- Visit the Aladdin Scientific product page and add rp364822 to your cart.
- Contact your regional Aladdin Scientific sales representative for bulk pricing, custom formulations, or institutional purchasing agreements.
- For technical questions regarding experimental design, assay compatibility, or troubleshooting, reach out to Aladdin Scientific technical support with your product lot number and datasheet reference (absea#PQ-524).
Aladdin Scientific offers reward points, promotional pricing, and flexible payment options. Please refer to current promotional terms on the website or contact customer service for details on coupons and credit programs.
Frequently asked questions
What is the biological function of GP5 (Glycoprotein V) on platelets?
GP5 (GPV/CD42d) is a subunit of the GPIb-IX-V complex on the platelet surface. This complex serves as the primary receptor for von Willebrand factor (vWF) under high shear stress, mediating initial platelet tethering to exposed subendothelium at sites of vascular injury. GP5 also plays a role in thrombin-mediated platelet activation, as thrombin can cleave the GP5 ectodomain.
What expression system is used to produce Recombinant Human GP5 Protein (rp364822)?
The specific expression system used for rp364822 should be confirmed in the product datasheet (absea#PQ-524), as recombinant proteins may be produced in mammalian, insect (baculovirus), E. coli, or yeast systems, each affecting glycosylation and folding. Please consult the datasheet or contact Aladdin Scientific technical support for this information.
How should I store Recombinant Human GP5 Protein to maintain its activity?
To preserve protein activity, store Recombinant Human GP5 Protein at -20°C for short-term use or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by aliquoting the protein upon first thaw. Always refer to the specific storage instructions provided in the product datasheet (absea#PQ-524).
Is Recombinant Human GP5 Protein suitable for use in cell-based functional assays?
Recombinant Human GP5 Protein may be used in functional assays, but suitability depends on purity, endotoxin levels, and the specific assay format. Endotoxin content is particularly critical for cell-based assays to avoid non-specific inflammatory responses. Consult the product datasheet for validated applications and endotoxin specifications before use.
Can I use Recombinant Human GP5 Protein for diagnostic or therapeutic purposes?
No. Recombinant Human GP5 Protein (rp364822) is designated for research use only (RUO) and is not approved or validated for diagnostic, clinical, or therapeutic use in humans. Any application outside of in vitro research requires appropriate regulatory evaluation and approval.
How do I request a Certificate of Analysis (CoA) for rp364822?
Certificates of Analysis (CoA) for Recombinant Human GP5 Protein (rp364822) can be requested through the Aladdin Scientific product page by entering your lot number, or by contacting Aladdin Scientific customer service directly. Safety Data Sheets (SDS) are also available upon request.
References
- Berndt MC, Shen Y, Dopheide SM, Gardiner EE, Andrews RK. The vascular biology of the glycoprotein Ib-IX-V complex. Thrombosis and Haemostasis. 2001;86(1):178-188. PMID:11487006
- Lanza F. Bernard-Soulier syndrome (hemorrhagiparous thrombocytic dystrophy). Orphanet Journal of Rare Diseases. 2006;1:46. doi:10.1186/1750-1172-1-46
- Andrews RK, Berndt MC. Platelet physiology and thrombosis. Thrombosis Research. 2004;114(5-6):447-453. PMID:15507271
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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