计算溶液所需的质量、体积或浓度。
BioReagent,for western blot,用于蛋白分析 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Tris-Glycine Transfer Buffer (pH 8.3, 10×) is a buffer reagent used for wet and semi-dry electrophoretic transfer in Western blotting. This product is prepared according to conventional methods and is a 10× concentrated solution. It contains no SDS (Sodium Dodecyl Sulfate) or methanol, making it easy to store and handle.This product is a 10× concentrated solution. Dilute it following the steps below before use:
1. Take 100 mL of 10× Tris-Glycine Transfer Buffer and add 200 mL of anhydrous methanol, then mix well.
2. Add distilled water or deionized water to bring the total volume to 1 L. After mixing well, the solution is ready for use.
3. The newly prepared 1× Tris-Glycine Transfer Buffer working solution can be stored at room temperature for approximately two weeks.
4. This product does not contain SDS. When the target protein has a large molecular weight or high hydrophobicity, a small amount of SDS can be added appropriately to improve the transfer effect (the recommended working concentration is 0.025-0.1%).
| pH | pH8.3 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | T670013 | |
| 分析证书 | T670013 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 for Protein analysis 级别指南 → 查看 for Western blot 级别指南 →