计算溶液所需的质量、体积或浓度。
红细胞裂解液 (10×)
有货
为什么选择此级别
BioReagent,无菌过滤,用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Red Blood Cell Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution based ammonium chloride, and should be diluted in deionized water prior to use.
Instructions for Use：
Lysis of Human Peripheral Blood:
1. Dilute the 10X concentrate 1:10 with distilled water to prepare the 1x lysis buffer. Warm the 1X solution to room temperature prior to use.
2. Add 20 ml of 1X RBC Lysis Buffer to each tube containing up to 1mL of whole blood.
3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature for 10-15 minutes.
4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing pellet.
5. Resuspend the pellet in the appropriate buffer, e.g. cell staining buffer
6. Resuspend and proceed with further procedures.
Lysis of Mouse Spleen:
1. Harvest mouse spleen and prepare a single cell suspension.
2. Pellet the cells by centrifugation and aspirate the supernatant.
3. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water and resuspend the pellet in 5 ml of 1X Lysis Buffer. Incubate on ice for 4-5 minutes.
4. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS.
5. Centrifuge 350 x g for 5 minutes and discard the supernatant.
6. Resuspend the pellet in the appropriate buffer, e.g. cell staining buffer.
7. Count cells, adjust density, and proceed with cell staining procedures.
规格
别名
红细胞裂解液
英文别名
RBC Lysis Buffer | Lysis Buffer
规格或纯度
无菌过滤, BioReagent, 用于细胞培养, 10x
稳定性与储存
Store at 2-8°C for 12 months. Do not freeze.
英文名称
Red Blood Cell Lysis Buffer (10×)
储存条件
2-8°C储存
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
产品属性
| pH | 7.4 |
|---|
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0939627 |
分析证书 | A1372415 |
| ZJ26F0939517 |
分析证书 | A1372415 |
| ZJ26F0636506 |
分析证书 | A1372415 |
| ZJ25F0724770 |
分析证书 | A1372415 |
| ZJ26F0434831 |
分析证书 | A1372415 |
| ZJ25F1129793 |
分析证书 | A1372415 |
技术文档和文章
无菌过滤
目录： 阿拉丁试剂的规格、级别和纯度
用于细胞培养
目录： 阿拉丁试剂的规格、级别和纯度
溶液计算器
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