活化蛋白C (APC)

CAS: 925910-74-3 货号: A1440682
有货
级别和纯度: 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 天然 ? 天然型(Native)—— 处于天然(非重组、非变性)形态的蛋白/生物分子。当需要天然结构和活性时使用。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 EnzymoPure™ ? EnzymoPure™ —— 阿拉丁的高质量酶解决方案系列。当酶纯度和明确活性决定检测或工艺性能时使用。 来源于人血浆; 冻干前浓度:见COA
生物活性
见COA
★
规格
库存
价格
数量
10μg
A1440682-10μg
现货 Stock Image
¥1,499.90
25μg
A1440682-25μg
现货 Stock Image
¥2,999.90
100μg
A1440682-100μg
现货 Stock Image
¥6,599.90
1mg
A1440682-1mg
期货 Stock Image
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概述


Activated Protein C (APC) is a serine protease derived from the two chain vitamin K dependent zymogen, Protein C. APC inhibits blood coagulation through the selective inactivation of the cofactors Va and VIIIa. APC is prepared from protein C by activation with purified thrombin. This thrombin is removed after activation by ion exchange chromatography. Activated Protein C purity is determined by SDS-PAGE and shows complete reduction upon incubation with 2-mercaptoethanol.

Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/ pH 7.4

Extinction Coefficient (1%) = 14.5

Molecular Weight = 56,000 daltons

The above protein was purified from Human plasma units that test negative for antibodies to HBc, HCV, HIV 1/2, HTLV-I/II, and non reactive for HBsAg, HIV-1 RNA, HCV RNA, HBV DNA and Syphilis by FDA approved tests. Donors are screened for CJD.

规格

产品名称
活化蛋白C (APC)
英文别名
Human Activated Protein C | Human Activated Protein C Native Protein | Vitamin K-dependent protein C | protein C, inactivator of coagulation factors Va and VIIIa | PROC | PC | APC | PROC1 | THPH3 | THPH4
规格或纯度
生物活性, ActiBioPure™, 天然, 高性能, EnzymoPure™, 来源于人血浆; 冻干前浓度:见COA
生化机理
Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function. Miscellaneous: Calcium also binds, with stronger affinity to another site, beyond the GLA domain. This GLA-independent binding site is necessary for the recognition of the thrombin-thrombomodulin complex. Post-translational: Carboxylated by vitamin K-dependent GGCX to form gamma-carboxyglutamate; these residues are essential for the binding of calcium. N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
生物活性
见COA
种属
人(Human)
来源
天然
预测分子量
56 kDa
CAS编号和信息
925910-74-3
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20 mM Tris-HCl, 0.1 M NaCl, pH 7.4.
浓度
来源于人血浆; 冻干前浓度:见COA
复溶
We suggest hydrating the protein with a good quality water to the original volume (See COA). After hydration aliquot into a useful (one time use) size and freeze at -80℃.
储存条件
2-8°C储存
运输条件
低温运输
稳定性与储存
长期储存2-8℃;复溶后建议分装,避免反复冻融。

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批号(Lot Number) 证书类型 货号
ZJ26F0737792ZJ26F0737792 分析证书 A1440682
ZJ26F0737791ZJ26F0737791 分析证书 A1440682
ZJ26F0737790ZJ26F0737790 分析证书 A1440682
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