In enzymology, an alcohol oxidase (EC 1.1.3.13) is an enzyme that catalyzes the chemical reaction:a primary alcohol + O₂↔ an aldehyde + H₂O₂. Thus, the two substRates of this enzyme are primary alcohol and O2, whereas its two products are aldehyde and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. It employs one cofactor, FAD. Applications Alcohol Oxidase may be used to study protein translocation into peroxisomes. This product is from Pichia pastoris. It has been used for the bacterial expression and immunological verification of Hv-p68 cDNA clones.
天然, EnzymoPure™, ≥20U/mg protein, ≥10U/mg powder; from Pichia pastoris
生化机理
Major isoform of alcohol oxidase, which catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of methylotrophic yeasts.
生物活性
≥20U/mg protein, ≥10U/mg powder
种属
Pichia pastoris
CAS编号和信息
9073-63-6
酶学委员会编号
EC 1.1.3.13
分子类型
酶
储存与运输
物理形态
冻干(Lyophilized)
浓度
≥20U/mg protein, ≥10U/mg powder; from Pichia pastoris
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
储存2-8℃(1年);长期储存-20℃(3年);复溶后建议分装,避免反复冻融。
单位定义
One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25°C.