淀粉样物质是一种无固定形状的细胞外嗜酸性物质，可存在于不同的组织、器官,导致的疾病称为淀粉样变；淀粉样物质主要是由蛋白质构成，该蛋白大部分排列成反向的β-折叠层结构。在电子显微镜下，淀粉样物质呈原纤维排列，病例材料中为大量细胞外的、不分支的细丝，大多随机排列。用于识别淀粉样物质的组织学方法有甲紫染色、刚果红染色、偏振光显微镜观察等，目前研究发现传统的甲紫染色法灵敏度低、特异性差，经典的而且有效的方法是刚果红染色，1922年Bennhold发现了刚果红可以用于活体内淀粉样物质的鉴别，并应用到组织切片，后来经过Highman改良，染色效果更好。

淀粉样物质染色液(甲紫法)主要由甲紫染色液、酸性分化液组成，是经Jurgens改良的一种异色染色液，其染色原理是蛋白样物质中的酸性黏多糖与甲紫起异色反应，其优点是简便省时，其缺点是染色后的切片难以保存。该试剂仅用于科研领域，不适用于临床诊断或其他用途。

A1508465	Component	2×50 mL	Storage
A1508465A	甲基紫染色液	50 mL	RT. Store in the dark.
A1508465B	酸性分化液	50 mL	RT

自备材料：

10%中性福尔马林、蒸馏水、系列乙醇、二甲苯或环保浸蜡脱蜡透明液、甘油明胶

操作步骤(仅供参考)：

1、常规固定，常采用10%中性福尔马林固定液，常规脱水包埋。

2、切片厚度4μm，常规二甲苯或脱蜡透明液脱蜡至水。

3、入甲基紫染色液3min，无需水洗。

4、滴加酸性分化液分化，直至无染色液脱出。

5、稍水洗，甘油明胶封固。

染色结果：

淀粉样物质: 红色至紫红色

细胞核、细胞质、结缔组织: 蓝色至深浅不一的紫蓝色

注意事项：

1、切片脱蜡应尽量干净，否则影响染色效果。

2、尽量采用浸染，如果滴染，应置于湿盒防止溶液挥发。

3、酸性分化液应密闭保存，分化步骤很重要。

4、染片不能经乙醇脱水，否则易出现无法上色的问题。

5、粘液物质甲紫染色时也会呈异染性红色，应注意鉴别。

6、在镜下观察异染性反应时，应注意把蓝色滤光片移去。

7、为了您的安全和健康，请穿实验服并戴一次性手套操作。

8、试剂开封后请尽快使用，以防影响后续实验效果。

Amyloid is an amorphous extracellular eosinophilic substance that can be present in various tissues and organs, and the diseases caused by it are referred to as amyloidosis. Amyloid is mainly composed of proteins, most of which are arranged in an antiparallel β-pleated sheet structure. Under an electron microscope, amyloid exhibits a fibrillary arrangement, appearing as a large number of unbranched extracellular filaments in pathological specimens, mostly arranged randomly.

Histological methods for identifying amyloid include methyl violet staining, Congo red staining, and polarized light microscopy. Current research has demonstrated that the traditional methyl violet staining method has low sensitivity and poor specificity, while Congo red staining is a classic and effective approach. In 1922, Bennhold discovered that Congo red could be used for the identification of amyloid in vivo and applied this technique to tissue sections. Later, the method was modified by Highman, resulting in improved staining efficacy.

Amyloid Staining Solution (Methyl Violet Method) is mainly composed of Methyl Violet Staining Solution and Acid Differentiating Solution. It is a metachromatic staining solution modified by Jurgens. Its staining principle is that the acid mucopolysaccharides in amyloid react metachromatically with methyl violet. The advantages of this method are simplicity and time-saving, while its disadvantage is that the stained sections are difficult to preserve. This reagent is for research use only and is not suitable for clinical diagnosis or any other purposes.

A1508465	Component	2×50 mL	Storage
A1508465A	Methyl Violet Staining Solution	50 mL	RT. Store in the dark.
A1508465B	Acid Differentiating Solution	50 mL	RT

Materials to be Prepared by User

10% neutral formalin, distilled water, graded ethanol, xylene or environment-friendly paraffin removal and clearing solution, glycerin gelatin

Procedure (for reference only)

1. Perform routine fixation, usually with 10% neutral formalin fixative, followed by routine dehydration and embedding.

2. Cut sections with a thickness of 4 μm, and perform routine dewaxing to water using xylene or dewaxing and clearing solution.

3. Immerse the sections in Methyl Violet Staining Solution for 3 min without rinsing with water.

4. Add Acid Differentiating Solution dropwise for differentiation until no more stain elutes out.

5. Rinse the sections slightly with water and mount them with glycerin gelatin.

Staining Results

Amyloid: Red to purplish red

Cell nuclei, cytoplasm, connective tissue: Blue to purple-blue of varying shades

Precautions

1. Ensure thorough dewaxing of sections; otherwise, the staining effect will be affected.

2. Prefer immersion staining; if drop staining is adopted, place the sections in a moist chamber to prevent solution evaporation.

3. Store Acid Differentiating Solution in a sealed container. The differentiation step is critical for staining quality.

4. Do not dehydrate the stained sections with ethanol, otherwise, the problem of failure to stain may occur.

5. Mucous substances also show metachromatic red color when stained with methyl violet, so attention should be paid to differentiation.

6. When observing the metachromatic reaction under the microscope, the blue filter should be removed.

7. For your safety and health, wear a lab coat and disposable gloves during operation.

8. Use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments.

规格

别名

淀粉样物质染色液

英文别名

Amyloid Staining Solution

规格或纯度

BioReagent,用于显微镜,生物染色剂

稳定性与储存

各组分在相应的储存条件下保质期1年。

英文名称

Amyloid Staining Solution (Crystal Violet Method)

储存条件

避光,室温

运输条件

常规运输

名称和识别符

分子类型

生物试剂/缓冲液

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批号(Lot Number)	证书类型	货号
ZJ26F0737385	分析证书	A1508465

技术文档和文章

a1508465 淀粉样物质染色液中文说明书.pdf

淀粉样物质刚果红染色中Highman、Bennhold、Puchtler及改良法的应用区别

目录：技术文章

探索主题:

刚果红染色淀粉样物质 Highman法 Bennhold法 Puchtler碱性刚果红法改良Highman法改良Stores法偏振光苹果绿色双折光组织病理染色

Application Differences Among Highman, Bennhold, Puchtler, and Modified Methods in Congo Red Staining for Amyloid

目录：技术文章

探索主题:

Congo red staining amyloid Highman method Bennhold method Puchtler alkaline Congo red method modified Highman method modified Stores metho polarized light apple-green birefringence histopathological staining

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淀粉样物质染色液 (甲紫法)

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