分枝杆菌的细胞壁内含有大量脂质包围在肽聚糖的外面,所以分枝杆菌一般不易着色，传统的染色方法要经过加热和延长染色时间来促使其着色。分枝杆菌中的分枝菌酸与染料一旦结合后，就很难被酸性脱色液脱色，故名抗酸染色，其中最具代表性是结核杆菌Ziehl－Neelsen染色法，该法是WHO推荐热染的方法。
阿拉丁抗酸染色液 (金胺O-罗丹明荧光法) 属于荧光染色液，无需加热，相对较抗酸热染液安全，其染色原理是在室温条件下AuramineO-罗丹明染色以及复染后，用含有紫外光源的荧光显微镜检查，抗酸杆菌呈亮黄色，而其他细菌及背景中的物质呈暗黄色，这种方法可用低倍镜检，因此能更快速找出抗酸性菌。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
A1510396
| Component
| 4×100mL
| Storage
|
A1510396A
| 金胺O-罗丹明染色液
| 100mL
| RT. Store in the dark.
|
A1510396B
| 酸性脱色液
| 2×100mL
| RT
|
A1510396C
| 复染液
| 100mL
| RT. Store in the dark. |
自备材料：
接种环、载玻片、蒸馏水、荧光显微镜
操作步骤(仅供参考)：
1、接种环挑取待检样本，涂布于载玻片上，加热固定。
2、滴加金胺O-罗丹明染色液避光染色30min，水洗。
3、用酸性脱色液脱色3min，直至涂片无黄色为止，水洗。
4、用复染液染色2min，水洗。轻轻吸干水分，自然干燥。
5、荧光显微镜下镜检。
染色结果：
注意事项：
1、每次使用后盖紧试剂瓶，以防试剂挥发和污染。
2、金胺O荧光易衰减，尽量避光操作。
3、上述试剂均对人体有刺激性，请注意适当防护。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
5、试剂开封后请尽快使用，以防影响后续实验效果。
The cell wall of Mycobacterium contains abundant lipids that surround the peptidoglycan layer, which generally renders Mycobacterium difficult to stain. Conventional staining methods require heating and extended staining time to facilitate dyeing of the bacteria. Once mycolic acid in Mycobacterium binds to the dye, it is difficult to decolorize with acid decolorizing solution, hence the name acid-fast staining. The most representative protocol is the Ziehl-Neelsen staining method for Mycobacterium tuberculosis, which is the recommended hot-staining method by the World Health Organization (WHO).
Aladdin Antacid Stain Solution (Auramine O-Rhodamine Fluorescence Method) is a type of fluorescent staining solution that does not require heating and is relatively safer than acid-fast hot-staining solutions. Its staining principle is as follows: after staining with Auramine O-Rhodamine and counterstaining at room temperature, the specimen is examined under a fluorescence microscope equipped with an ultraviolet light source. Acid-fast bacilli appear bright yellow, while other bacteria and substances in the background appear dark yellow. This method allows examination under low magnification, enabling faster detection of acid-fast bacilli. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
A1510396
| Component
| 4×100mL
| Storage
|
A1510396A
| Auramine O-Rhodamine Staining Solution
| 100mL
| RT. Store in the dark.
|
A1510396B
| Acid Decolorizing Solution
| 2×100mL
| RT
|
A1510396C
| Counterstain Solution
| 100mL
| RT. Store in the dark. |
Self-prepared Materials:
Inoculation loop, glass slides, distilled water, fluorescence microscope
Operating Procedures (For Reference Only):
1. Pick the sample to be tested with an inoculation loop, spread it evenly on a glass slide, and fix it by heating.
2. Add a sufficient amount of Auramine O-Rhodamine Staining Solution to cover the smear, stain for 30 minutes in the dark, and rinse with water.
3. Decolorize with Acid Decolorizing Solution for 3 minutes until the smear no longer shows yellow color, then rinse with water.
4. Stain with Counterstain Solution for 2 minutes, rinse with water, blot the excess water gently, and air-dry naturally.
5. Observe the prepared slide under a fluorescence microscope.
Staining Results:
Acid-fast bacilli
| Bright yellow or orange-yellow
|
Non-acid-fast bacilli, cells, background
| Dark yellow
|
Precautions:
1. Tighten the reagent bottle caps immediately after each use to prevent reagent volatilization and contamination.
2. The fluorescence of Auramine O fades easily; please perform all operations as much as possible away from light.
3. All the above-mentioned reagents are irritating to the human body; please take appropriate protective measures.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
5. Please use the reagents as soon as possible after opening to avoid affecting the results of subsequent experiments.
ZJ26F0333473
危险品化学品经营许可证（带存储）