分枝杆菌的细胞壁内含有大量脂质包围在肽聚糖的外面，所以分枝杆菌一般不易着色。传统的染色方法要经过加热和延长染色时间来促使其着色。分枝杆菌中的分枝菌酸与染料一旦结合后，就很难被酸性脱色液脱色，故名抗酸染色。其中最具代表性是结核杆菌Ziehl－Neelsen染色法，该法是WHO推荐热染的方法。
阿拉丁抗酸染色液 (Kinyoun冷染法) 属于冷染色液，无需加热，相对较抗酸热染液安全。其染色原理是在室温条件下，分枝菌酸不复红结合成复合物，经亚甲蓝复染后，分枝杆菌仍然为红色，而其他细菌及背景中的物质为蓝色。该染色法较改良Kinyoun冷染法要深，更适用于常规抗酸染色，推荐该Kinyoun冷染法作为实验室或临床上标准抗酸染色法。
产品组分及储存条件：
A1510404
| Component
| 3×50mL
| 3×100mL
| Storage
|
A1510404A
| Kinyoun 复红染色液
| 50mL
| 100mL
| RT. Store in the dark.
|
A1510404B
| Kinyoun 脱色液
| 50mL
| 100mL
| RT
|
A1510404C
| 亚甲蓝染色液
| 50mL
| 100mL
| RT. Store in the dark. |
自备材料：
1、接种环
2、载玻片
3、蒸馏水
4、显微镜
操作步骤(仅供参考)：
1、接种环挑取待检样本，涂布于载玻片上，自然干燥。
2、滴加Kinyoun复红染色液，染色5～10min。
3、不必加热，蒸馏水冲洗。
4、用Kinyoun脱色液脱色至无红色为止。
5、蒸馏水冲洗。
6、用亚甲蓝染色液染色30~60s。
7、蒸馏水冲洗。
8、轻轻吸干水分，自然干燥。
6、用亚甲蓝染色液染色30~60s。
7、蒸馏水冲洗。
8、轻轻吸干水分，自然干燥。
9、油镜镜检。
注意事项：
1、每次使用后盖紧瓶盖，以防试剂挥发和污染。
2、上述试剂均对人体有刺激性，请注意适当防护。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作。
4、本产品仅供科研使用，严禁它用。
The cell wall of mycobacteria contains a large amount of lipids that surround the peptidoglycan layer, so mycobacteria are generally difficult to stain. Traditional staining methods require heating and prolonged staining time to promote dyeing. Once mycolic acid in mycobacteria combines with the dye, it is difficult to be decolorized by acid decolorizing solution, hence the name acid-fast staining. One of the most representative methods is the Ziehl-Neelsen staining method for Mycobacterium tuberculosis, which is the heat staining method recommended by the WHO.
Aladdin Acid-fast Stain Solution (Kinyoun Cold Staining Method) is a cold stain solution that does not require heating and is relatively safer than acid-fast heat stain solutions. Its staining principle is that at room temperature, mycolic acid combines with fuchsin to form a complex. After counterstaining with methylene blue, mycobacteria remain red, while other bacteria and substances in the background turn blue. This staining method results in a darker color than the modified Kinyoun cold staining method and is more suitable for routine acid-fast staining. The Kinyoun cold staining method is recommended as the standard acid-fast staining method for laboratories or clinical settings.
Product Components and Storage Conditions:
A1510404
| Component
| 3×50mL
| 3×100mL
| Storage
|
A1510404A
| Kinyoun Fuchsin Stain Solution
| 50mL
| 100mL
| RT. Store in the dark.
|
A1510404B
| Kinyoun Decolorizing Solution
| 50mL
| 100mL
| RT
|
A1510404C
| Methylene Blue Stain Solution
| 50mL
| 100mL
| RT. Store in the dark. |
Self-prepared Materials:
1. Inoculating loop
2. Glass slide
3. Distilled water
4. Microscope
Operating Procedures (For Reference Only):
1. Pick the test sample with an inoculating loop, spread it evenly on the glass slide, and air-dry naturally.
2. Add a drop of Kinyoun Fuchsin Stain Solution and stain for 5–10 minutes.
3. No heating required. Rinse the slide with distilled water.
4. Decolorize with Kinyoun Decolorizing Solution until no red color is visible.
5. Rinse with distilled water.
6. Stain with Methylene Blue Stain Solution for 30–60 seconds.
7. Rinse with distilled water.
8. Blot the water gently and air-dry naturally.
9. Examine under the oil immersion lens of the microscope.
Precautions:
1. Tighten the bottle caps immediately after each use to prevent reagent volatilization and contamination.
2. All the above reagents are irritating to the human body; please take appropriate protective measures.
3. For your safety and health, wear a lab coat and disposable gloves during operation.
4. This product is for research use only. Any other applications are strictly prohibited.
ZJ26F0332975
危险品化学品经营许可证（带存储）