计算溶液所需的质量、体积或浓度。
碱性固绿染色液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
不同的氨基酸带有不同化学性质的侧链基团，有的带有碱性侧链，有的带有酸性侧链，由此组成的蛋白质具有不同数目的碱性基团和酸性基团，这些基团会使蛋白质在不同的pH溶液中带有不同的净电荷，整个蛋白质分子带正电荷多，即为碱性蛋白(等电点偏向酸性)；整个蛋白质带负电荷多，即为酸性蛋白质(等电点偏向酸性)。利用蛋白质的两重性(酸碱性)及与酸性和碱性染料离子形成盐的能力，在 pH=2.2（即所有蛋白质等电点之下），全部蛋白质能被固绿染色；在 pH=8.1~8.2 范围内，碱性蛋白质被固绿染色。
碱性固绿染色液是利用碱性蛋白质与带有负电荷的碱性染料固绿结合进行染色，细胞中含量最为丰富的碱性蛋白为组蛋白，主要存在于细胞核中，因此染色后细胞核大部分被染成绿色。该染色液仅用于科研领域，不宜用于临床诊断或其他用途。
产品组分及储存条件：
| A1511546 | Component | 2×50mL | Storage |
| A1511546A | 固绿染色液 | 50mL | RT. Store in the dark. |
| A1511546B | 碱性缓冲液 | 50mL | RT |
自备材料：
玻片、水浴锅、显微镜、pH 计、70%乙醇、中性树胶、稀氢氧化钠或稀盐酸、5%三氯乙酸
操作步骤(仅供参考)：
配制碱性固绿染色液：将固绿染色液和碱性缓冲液等比例混合，用pH计测定，若pH在8.0~8.5 之内，可直接使用，若偏离此范围，可根据情况调整后使用；此染色液配制后不可久置。
1、石蜡切片脱蜡至水。
2、浸入 5%三氯乙酸中，90℃水浴 15min，抽提除去核酸。
3、流水稍冲洗，70%乙醇洗涤三次，每次 3min；滤纸吸去残留水分。
4、涂片浸入碱性固绿染色液染色 20～30min。
5、流水冲洗 5min，95%乙醇 30s，无水乙醇两次、每次 1min。
6、二甲苯或脱蜡透明液两次、1min，中性树胶封片。镜检。
染色结果：
细胞核大部分区域：绿色
细胞质、核仁：不着色
注意事项：
1、血液涂片或骨髓涂片应厚薄均匀，以免影响染色效果。
2、血细胞涂片染色要求新鲜全血或EDTA抗凝血。
3、染色过深可用甲醇或酒精适当脱色，最好不复染。
4、pH值对染色有一定影响，载玻片应清洁、无酸碱污染，以免影响染色效果。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。。
6、本产品仅供科研使用，严禁它用。
Different amino acids carry side chain groups with different chemical properties, some with basic side chains and others with acidic side chains. Proteins composed of these amino acids therefore contain different numbers of basic and acidic groups. These groups cause proteins to carry different net charges in solutions of different pH values. A protein molecule with a net positive charge is referred to as a basic protein (with an isoelectric point biased toward the acidic range); a protein with a net negative charge is referred to as an acidic protein (with an isoelectric point biased toward the acidic range). Taking advantage of the amphoteric nature (acidity and basicity) of proteins and their ability to form salts with acidic and basic dye ions: At pH 2.2 (below the isoelectric point of all proteins), all proteins can be stained by Fast Green. In the range of pH 8.1–8.2, basic proteins are stained by Fast Green.
Basic Fast Green Staining Solution stains by the binding between basic proteins and negatively charged basic dye Fast Green. The most abundant basic protein in cells is histone, which is mainly located in the cell nucleus. Therefore, most of the cell nucleus is stained green after staining. This staining solution is for research use only and is not suitable for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
| A1511546 | Component | 2×50mL | Storage |
| A1511546A | Fast Green Staining Solution | 50mL | RT. Store in the dark. |
| A1511546B | Alkaline Buffer | 50mL | RT |
Materials Required (User-supplied):
Glass slides, water bath, microscope, pH meter, 70% ethanol, neutral balsam, dilute sodium hydroxide or dilute hydrochloric acid, 5% trichloroacetic acid
Protocol (For Reference Only):
Preparation of Basic Fast Green Staining Solution: Mix Fast Green Staining Solution and Alkaline Buffer in equal proportions. Measure with a pH meter. If the pH is within 8.0–8.5, the solution can be used directly; if outside this range, adjust the pH before use. The prepared staining solution cannot be stored for a long time.
1. Deparaffinize paraffin sections and bring to water.
2. Immerse in 5% trichloroacetic acid, incubate in a water bath at 90 °C for 15 min to extract and remove nucleic acids.
3. Rinse briefly with running water, then wash three times in 70% ethanol, 3 min each time. Blot excess moisture with filter paper.
4. Stain in Basic Fast Green Staining Solution for 20–30 min.
5. Rinse in running water for 5 min, treat with 95% ethanol for 30 s, then absolute ethanol twice, 1 min each time.
6. Clear twice in xylene or deparaffinizing clearing solution, 1 min each. Mount with neutral balsam and examine microscopically.
Staining Results:
Most regions of the cell nucleus: Green
Cytoplasm, nucleoli: Unstained
Precautions:
1. Blood smears or bone marrow smears should be of uniform thickness to avoid affecting the staining result.
2. Fresh whole blood or EDTA-anticoagulated blood is required for blood cell smear staining.
3. If overstained, appropriate decolorization can be performed with methanol or ethanol; restaining is not recommended.
4. pH value has a certain influence on staining. Glass slides should be clean and free from acid or alkali contamination to avoid affecting the staining result.
5. For your safety and health, please wear a lab coat and disposable gloves during operation.
6. This product is for research use only, strictly prohibited for other purposes.
规格
别名
碱性蛋白染色液
英文别名
Basic Fast Green Stain | Alkaline Solid Green Stain set
规格或纯度
BioReagent,生物染色剂,用于显微镜
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Basic Fast Green Staining Solution
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434253 |
分析证书 | A1511546 |
技术文档和文章
溶液计算器
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