BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
淀粉酶（Amylase，AMS），又称1,4-α-D-葡聚糖水解酶，是能够催化淀粉与糖原水解的一类酶的总称。根据检测底物不同，其测定方法主要分为两类：一是以天然淀粉为底物的方法（如本试剂盒采用的碘-淀粉法）；二是使用确定结构底物的方法（如以麦戊糖或4-硝基苯麦芽糖苷（4-NP-G）为底物的方法）。 本试剂盒基于经典的碘-淀粉比色原理。样本中的α-淀粉酶催化淀粉分子中的α-1,4糖苷键水解，生成葡萄糖、麦芽糖及糊精等产物。反应体系中，碘液与未被水解的淀粉结合形成蓝色复合物。α-淀粉酶活性越高，剩余的淀粉越少，蓝色则越浅。通过酶标仪在660 nm波长下检测吸光度，与空白对照比较，即可计算出样本中淀粉酶的活力单位。 本产品适用于检测细胞或组织裂解液/匀浆液、血浆、血清、尿液等多种生物样品中的内源性淀粉酶活性。仅限用于科学研究，不适用于临床诊断或其他用途。
Amylase (AMS), also known as 1,4-α-D-glucan hydrolase, is a general term for a class of enzymes capable of catalyzing the hydrolysis of starch and glycogen. Based on the substrate used for detection, its assay methods are primarily divided into two categories: one employs natural starch as the substrate (such as the iodine-starch method adopted by this kit), and the other utilizes defined substrates (such as maltopentaose or 4-nitrophenyl maltoside (4-NP-G)). This kit is based on the classic iodine-starch colorimetric principle. The α-amylase in the sample catalyzes the hydrolysis of the α-1,4-glycosidic bonds in starch molecules, producing glucose, maltose, dextrins, and other products. In the reaction system, iodine solution binds to the unhydrolyzed starch to form a blue complex. The higher the α-amylase activity, the less starch remains, resulting in a lighter blue color. By measuring the absorbance at 660 nm using a microplate reader and comparing it with a blank control, the amylase activity units in the sample can be calculated. It is suitable for detecting endogenous amylase activity in various biological samples such as cell or tissue lysates/homogenates, plasma, serum, and urine. This product is intended for research use only and is not suitable for clinical diagnosis or other purposes.
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