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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
淀粉酶（Amylase，AMS），亦称1,4-α-D-葡聚糖水解酶，是一类能够催化淀粉与糖原水解的酶的总称。其活性测定方法主要分为两类：一类是以天然淀粉为底物的方法，如碘-淀粉法；另一类则是采用确定结构底物的方法，例如以麦戊糖或4-硝基苯麦芽糖苷（4-NP-G）为底物的检测体系。 本试剂盒基于碘-淀粉比色原理：样本中的α-淀粉酶催化淀粉分子中的α-1,4糖苷键水解，生成葡萄糖、麦芽糖及糊精等产物；反应后剩余的淀粉与碘液结合形成蓝色复合物，其颜色深浅与酶活性成反比。通过分光光度计在660 nm波长处测定吸光度，并与空白对照比较，即可计算出淀粉酶的活力单位。 本品适用于细胞或组织裂解液/匀浆液、血浆、血清、尿液等样品中内源性淀粉酶活性的检测。仅限用于科研领域，不适用于临床诊断或其他用途。
Amylase (AMS), also known as 1,4-α-D-glucan hydrolase, is a general term for a class of enzymes capable of catalyzing the hydrolysis of starch and glycogen. Methods for measuring its activity are primarily divided into two categories: one uses natural starch as the substrate, such as the iodine-starch method; the other employs structurally defined substrates, such as detection systems based on maltopentaose or 4-nitrophenyl maltoside (4-NP-G). This kit is based on the iodine-starch colorimetric principle: α-amylase in the sample catalyzes the hydrolysis of the α-1,4-glycosidic bonds in starch molecules, producing glucose, maltose, dextrins, and other products. The remaining starch after the reaction combines with iodine solution to form a blue complex, the color intensity of which is inversely proportional to the enzyme activity. By measuring the absorbance at 660 nm using a spectrophotometer and comparing it with a blank control, the activity units of amylase can be calculated. This product is suitable for the detection of endogenous amylase activity in samples such as cell or tissue lysates/homogenates, plasma, serum, and urine. It is intended for research use only and is not suitable for clinical diagnosis or other purposes.
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