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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
淀粉酶（Amylase，AMS）又称1,4-α-D-葡聚糖水解酶，是催化淀粉与糖原水解的一类酶的总称，主要包括α-淀粉酶、β-淀粉酶与葡萄糖淀粉酶。其中，α-淀粉酶可随机切断淀粉链，生成麦芽糖、麦芽三糖及糊精等，同时降低体系黏度，故又称液化酶，β-淀粉酶则从淀粉非还原端依次切下单分子麦芽糖，因而被称为糖化酶。 本试剂盒基于3,5-二硝基水杨酸（NS）比色法：两种淀粉酶水解淀粉产生的麦芽糖在碱性条件下还原DNS，生成在540 nm处有特征吸收的棕红色化合物，通过测定吸光度可计算酶活力。利用β-淀粉酶不耐热的特性（70℃加热15分钟可被钝化），可先测定α-淀粉酶活力，再与未处理样本的总活力（α+β）比较，经差值计算得到β-淀粉酶活力。 本方法适用于植物或动物的组织/细胞裂解液、血浆、血清、尿液等样本的内源性淀粉酶活性检测，具有灵敏度与准确性高的特点，尤其适合微量样品中β-淀粉酶活性的精确分析，但操作步骤较多，不建议用于大批量样本的高通量筛选。本品仅限科研使用，不适用于临床诊断或其他用途。
Amylase (AMS), also known as 1,4-α-D-glucan hydrolase, is a general term for a class of enzymes that catalyze the hydrolysis of starch and glycogen. It primarily includes α-amylase, β-amylase, and glucoamylase. Among them, α-amylase randomly cleaves starch chains to produce maltose, maltotriose, dextrins, etc., while reducing the viscosity of the system, hence it is also called the liquefying enzyme. β-amylase sequentially cleaves single maltose molecules from the non-reducing ends of starch and is therefore referred to as the saccharifying enzyme. This kit is based on the 3,5-dinitrosalicylic acid (DNS) colorimetric method: the maltose produced by the hydrolysis of starch by both amylases reduces DNS under alkaline conditions, generating a brown-red compound with characteristic absorption at 540 nm. Enzyme activity can be calculated by measuring the absorbance. Utilizing the heat-sensitive property of β-amylase (which can be inactivated by heating at 70°C for 15 minutes), the activity of α-amylase is measured first. It is then compared with the total activity (α+β) measured in non-inactivated samples, and the β-amylase activity is obtained by difference calculation. This method is suitable for the detection of endogenous amylase activity in samples such as plant or animal tissue/cell lysates, plasma, serum, and urine. It offers high sensitivity and accuracy, making it particularly suitable for the precise analysis of β-amylase activity in small-volume samples. However, due to its multiple procedural steps, it is not recommended for high-throughput screening of large sample sets. This product is for research use only and is not intended for clinical diagnosis or other purposes.
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