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APX是植物清除活性氧的重要抗氧化酶之一，也是抗坏血酸代谢的关键酶之一。APX具有多种同功酶，分别定位于叶绿体、胞质、线粒体、过氧化物和乙醛酸体，以及过氧化体和类囊体膜上。APX 催化H₂O₂氧化AsA，是植物AsA 的主要消耗者。APX的活性直接影响到AsA的含量，APX与AsA具有一定的负相关性。 测定原理：APX 催化催化H₂O₂氧化AsA，通过测定AsA氧化速率，来计算得APX活性。
APX (Ascorbate Peroxidase) is one of the important antioxidant enzymes involved in scavenging reactive oxygen species in plants and is also a key enzyme in ascorbic acid metabolism. APX has multiple isoenzymes localized in chloroplasts, the cytosol, mitochondria, peroxisomes, glyoxysomes, as well as in the peroxisomal and thylakoid membranes. APX catalyzes the oxidation of AsA (ascorbic acid) by H₂O₂ and serves as a major consumer of AsA in plants. The activity of APX directly affects the AsA content, and there is a certain negative correlation between APX and AsA. Assay Principle: APX catalyzes the oxidation of AsA by H₂O₂. The activity of APX is calculated by measuring the rate of AsA oxidation.
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