计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
结缔组织狭义上是指其含有的三种纤维:胶原纤维、网状纤维、弹力纤维,而胶原纤维(collagen fiber)是分布最广、含量最多的一种纤维。Masson三色染色又称马松染色,是结缔组织染色中最经典的一种方法,是胶原纤维染色权威而经典的技术方法。所谓三色染色通常是指染胞核和能选择性的显示胶原纤维和肌纤维,该法染色原理与阴离子染料分子的大小和组织的渗透有关:分子的大小由分子量来体现,小分子量易穿透结构致密、渗透性低的组织;而大分子量则只能进入结构疏松的、渗透性高的组织。然而,淡绿或苯胺蓝的分子量都很大,因此Masson染色后肌纤维呈红色,胶原纤维呈绿色(淡绿)或蓝色(苯胺蓝),主要用于区分胶原纤维和肌纤维。
苯胺蓝染色液(0.1%)主要有苯胺蓝、弱酸等组成,常与丽春红品红染色液等配合使用对胶原纤维进行染色。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
1、根据实验具体要求操作。
2、一般浸染1~2min,可根据染色结果适当调整。
注意事项:
1、切片脱蜡应尽量干净。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
In a narrow sense, connective tissue contains three types of fibers: collagen fibers, reticular fibers, and elastic fibers. Among these, collagen fibers are the most widely distributed and abundant fiber type. Masson’s Trichrome Staining, also known as Masson staining, is the most classic method for connective tissue staining and an authoritative and standard technique for collagen fiber staining. Trichrome staining generally stains nuclei and selectively demonstrates collagen fibers and muscle fibers. The staining principle is related to the molecular size of anionic dyes and tissue permeability: Molecular size is reflected by molecular weight. Small molecules easily penetrate dense tissues with low permeability, whereas large molecules can only enter loose tissues with high permeability. Since light green or aniline blue has a large molecular weight, muscle fibers appear red and collagen fibers appear green (light green) or blue (aniline blue) after Masson staining. This method is mainly used to distinguish collagen fibers from muscle fibers.
Aniline Blue Staining Solution (0.1%) consists mainly of aniline blue and weak acid. It is often used in combination with Ponceau Fuchsin Staining Solution to stain collagen fibers. This reagent is for research use only. Not for clinical diagnosis or other purposes.
Operating Procedures (For Reference Only):
1. Perform the operation according to the specific experimental requirements.
2. Generally, immerse and stain for 1–2 minutes; the duration can be adjusted appropriately according to the staining results.
Precautions:
1. Section deparaffinization should be as thorough as possible.
2. For your safety and health, wear laboratory coat and disposable gloves during operation.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | A1516040 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
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