BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
转氨酶是催化α-氨基酸和α-酮酸之间氨基转换反应的一组酶，天门冬氨酸氨基转移酶(AST)旧称谷草转氨酶(GOT)主要存在于心肌、骨骼肌、肝脏，以心肌含量最高，肝脏次之，AST能够催化天门冬氨酸和a-酮戊二酸的氨基转移作用，形成谷氨酸和草酰乙酸。 该试剂盒检测原理是AST催化天门冬氨酸与α-酮戊二酸之间的氨基转移反应，其反应公式如下：L-天门冬氨酸+α-酮戊二酸→草酰乙酸+L-谷氨酸。 二硝基苯肼与α-酮酸反应生成相应的二硝基苯腙，在碱性条件下二硝基苯腙的吸收光谱有差异，检测在500-520nm处差异最大，以等摩尔浓度计算出丙酮酸的生成量，进而计算酶的活性。该试剂盒仅用于科研领域，不宜用于临床诊断或其他用途。
Transaminases are a group of enzymes that catalyze the transfer of amino groups between α-amino acids and α-keto acids. Aspartate aminotransferase (AST), formerly known as glutamic-oxaloacetic transaminase (GOT), is primarily found in the heart, skeletal muscle, and liver, with the highest content in the heart, followed by the liver. AST catalyzes the transfer of the amino group between aspartate and α-ketoglutarate, resulting in the formation of glutamate and oxaloacetate. The detection principle of this kit is based on the transamination reaction catalyzed by AST between aspartate and α-ketoglutarate, as shown in the following reaction formula: L-Aspartate + α-Ketoglutarate → Oxaloacetate + L-Glutamate. Dinitrophenylhydrazine reacts with α-keto acids to form the corresponding dinitrophenylhydrazone derivatives. Under alkaline conditions, the absorption spectra of these dinitrophenylhydrazone derivatives differ. The maximum difference in absorption is observed at 500–520 nm. The amount of pyruvate produced is calculated based on its equimolar concentration, and the enzyme activity is subsequently determined. This kit is intended for research purposes only and is not suitable for clinical diagnosis or other applications.
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