BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
转氨酶是催化α-氨基酸和α-酮酸之间氨基转换反应的一组酶，丙氨酸氨基转移酶(ALT)旧称谷丙转氨酶(GPT)主要存在于肝细胞浆内，细胞内ALT浓度远高于血清，肝细胞破坏后血清ALT立即迅速升高，因此谷ALT被世界卫生组织推荐为肝功能损害最敏感的检测指标。 该试剂盒检测原理是ALT催化丙氨酸与α-酮戊二酸之间的氨基转移反应，其反应公式如下：L-丙氨酸+α-酮戊二酸→丙酮酸+L-谷氨酸。 二硝基苯肼与α-酮酸反应生成相应的二硝基苯腙，在碱性条件下二硝基苯腙的吸收光谱有差异，检测在500~520nm处差异最大，以等摩尔浓度计算出丙酮酸的生成量，进而计算酶的活性。本产品仅用于科研领域，不宜用于临床诊断或其他用途。
Transaminases are a group of enzymes that catalyze the transfer of amino groups between α-amino acids and α-keto acids. Alanine aminotransferase (ALT), formerly known as glutamic-pyruvic transaminase (GPT), is primarily found in the cytoplasm of hepatocytes. The intracellular concentration of ALT is significantly higher than that in serum, and upon hepatocyte damage, serum ALT levels rise immediately and rapidly. Therefore, ALT is recommended by the World Health Organization as the most sensitive indicator for assessing liver damage. The detection principle of this kit is based on the transamination reaction catalyzed by ALT between alanine and α-ketoglutarate, as shown in the following reaction formula: L-Alanine + α-Ketoglutarate → Pyruvate + L-Glutamate. Dinitrophenylhydrazine reacts with α-keto acids to form the corresponding dinitrophenylhydrazone derivatives. Under alkaline conditions, the absorption spectra of these dinitrophenylhydrazone derivatives differ. The maximum difference in absorption is observed at 500–520 nm. The amount of pyruvate produced is calculated based on its equimolar concentration, and the enzyme activity is subsequently determined. This product is intended for research purposes only and is not suitable for clinical diagnosis or other applications.
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