E2529236计算溶液所需的质量、体积或浓度。
Aladdin Fluor 790 NHS Ester
有货
为什么选择此级别
Em:814nm,Ex:784nm 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Aladdin Fluor 790 is a bright and photostable near-IR dye that is spectrally similar to indocyanine green (ICG) and the IRDye™ 800 dye. Used for stable signal generation in imaging and flow cytometry, Aladdin Fluor 790 dye is water soluble and pH-insensitive from pH 4 to pH 10. Fluorescence of this long-wavelength Aladdin Fluor dye is not visible to the human eye but is readily detected by most imaging systems. As the longest-wavelength Aladdin Fluor dye, the emission is well separated from commonly used far-red fluorophores such as Aladdin Fluor 647 dye or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also expected to be useful for small animal in-vivo imaging (SAIVI) applications or for two-color western applications with the LI-COR™ Odyssey™ infrared imaging system. In addition to reactive dye formulations, we offer Aladdin Fluor 790 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection.The NHS ester (or succinimidyl ester) of Aladdin Fluor 790 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
Detailed information about this Aladdin Fluor NHS ester:
Fluorophore label: Aladdin Fluor 790 dye
Reactive group: NHS ester
Reactivity: Primary amines on proteins and ligands, amine-modified oligonucleotides
Ex/Em of the conjugate: 784/814 nm
Extinction coefficient: 260,000 cm-1M-1
Molecular weight: ∼1750
Typical Conjugation Reaction
You can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies). You can scale the reaction for any amount of protein, but the concentration of the protein should be at least 2 mg/mL for optimal results. We recommend trying three different degrees of labeling, using three different molar ratios of the reactive reagent to protein.
The Aladdin Fluor NHS ester is typically dissolved in high-quality anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO) , and the reaction is carried out in 0.1–0.2 M sodium bicarbonate buffer, pH 8.3, at room temperature for 1 hour. Because the pKa of the terminal amine is lower than that of the lysine epsilon-amino group, you may achieve more selective labeling of the amine terminus using a buffer closer to neutral pH.
Conjugate Purification
Labeled antibodies are typically separated from free Aladdin Fluor dye using a gel filtration column. For much larger or smaller proteins, select a gel filtration media with an appropriate molecular weight cut-off or purify by dialysis. We offer several purification kits optimized for different quantities of antibody conjugate:
Antibody Conjugate Purification Kit for 0.5-1 mg ()
Antibody Conjugate Purification Kit for 20-50 µg ()
Antibody Conjugate Purification kit for 50-100 µg ()
Detailed information about this Aladdin Fluor NHS ester:
Fluorophore label: Aladdin Fluor 790 dye
Reactive group: NHS ester
Reactivity: Primary amines on proteins and ligands, amine-modified oligonucleotides
Ex/Em of the conjugate: 784/814 nm
Extinction coefficient: 260,000 cm-1M-1
Molecular weight: ∼1750
Typical Conjugation Reaction
You can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies). You can scale the reaction for any amount of protein, but the concentration of the protein should be at least 2 mg/mL for optimal results. We recommend trying three different degrees of labeling, using three different molar ratios of the reactive reagent to protein.
The Aladdin Fluor NHS ester is typically dissolved in high-quality anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO) , and the reaction is carried out in 0.1–0.2 M sodium bicarbonate buffer, pH 8.3, at room temperature for 1 hour. Because the pKa of the terminal amine is lower than that of the lysine epsilon-amino group, you may achieve more selective labeling of the amine terminus using a buffer closer to neutral pH.
Conjugate Purification
Labeled antibodies are typically separated from free Aladdin Fluor dye using a gel filtration column. For much larger or smaller proteins, select a gel filtration media with an appropriate molecular weight cut-off or purify by dialysis. We offer several purification kits optimized for different quantities of antibody conjugate:
Antibody Conjugate Purification Kit for 0.5-1 mg ()
Antibody Conjugate Purification Kit for 20-50 µg ()
Antibody Conjugate Purification kit for 50-100 µg ()
规格
规格或纯度
Ex:784nm, Em:814nm
英文名称
Aladdin Fluor 790 NHS Ester
储存条件
避光,-20°C储存
运输条件
超低温运输
Ex/Em(nm)
Ex:782nm;Em:805nm
名称和识别符
分子类型
小分子
技术文档
高级数据
产品属性
| Reactivity | Amine |
|---|
化学和物理性质
敏感性
易吸潮
Excitation(nm)
784
Emission(nm)
814
质检证书（CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
溶液计算器
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