F2426333计算溶液所需的质量、体积或浓度。
Aladdin™ 546 NHS 酯（琥珀酰亚胺酯）
有货
为什么选择此级别
Em:572nm,Ex:561nm 级 提供 ≥70%(HPLC) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Aladdin™ 546 is a bright yellow dye. Used in imaging and flow cytometry, Aladdin™ 546 dye is water soluble and pH-insensitive from pH 4 to pH 10.
The NHS ester (or succinimidyl ester) of Aladdin™ 546 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting Aladdin™ conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.
Detailed information about this Aladdin™ NHS ester:
Fluorophore label: Aladdin™ 546 dye
Reactive group: NHS ester
Reactivity: Primary amines on proteins and ligands, amine-modified oligonucleotides
Ex/Em of the conjugate: 554/570 nm
Extinction coefficient: 112,000 cm-1M-1
Molecular weight: 1159.6
Typical Conjugation Reaction
You can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies). You can scale the reaction for any amount of protein, but the concentration of the protein should be at least 2 mg/mL for optimal results. We recommend trying three different degrees of labeling, using three different molar ratios of the reactive reagent to protein.
Aladdin™ NHS ester is typically dissolved in high-quality anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO), and the reaction is carried out in 0.1–0.2 M sodium bicarbonate buffer, pH 8.3, at room temperature for 1 hour. Because the pKa of the terminal amine is lower than that of the lysine epsilon-amino group, you may achieve more selective labeling of the amine terminus using a buffer closer to neutral pH.
规格
规格或纯度
Ex:561nm, Em:572nm, ≥70%(HPLC)
稳定性与储存
储存于-20℃（12个月），避光。
英文名称
Aladdin™ 546 NHS Ester(Succinimidyl Ester)
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
纯度
≥70%(HPLC)
Ex/Em(nm)
Ex:561nm;Em:572nm
产品线
生物产品
名称和识别符
分子类型
小分子
分子量
1058.4（free base）
技术文档
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