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，适用于对基线干扰要求严格的色谱和分析工作流程。
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概述
产品介绍：
Ad-mCherry-p62 ，即adenovirus expressing mCherry-p62 fusion protein，是一种可以表达mCherry-p62融合蛋白的腺病毒，可以用于感染细胞或组织后进行细胞自噬(autophagy)的检测。p62也称sequestosome I (SQSTM1)，在多细胞生物(不包括植物和真菌)中高度保守，主要分布于细胞质，也可以定位于细胞核、自噬体(autophagosome)和溶酶体(lysosome)中。p62是一种应激诱导的蛋白，可以作为信号枢纽(signaling hub)在氨基酸感应(amino acid sensing)和氧化应激等许多细胞事件中发挥重要作用，并且还可以作为PKC、ERK1、mTORC1、NF-кB和caspase-8等的支架蛋白而参与相应的信号转导。p62全长440个氨基酸，包含一个PB1(Phox1/Bem1p)结构域，一个锌指结构域(ZZ)，两个核定位信号(NLS1和NLS2)，一个TB (TRAF6 binding)结构域，一个LIR结构域(LC3-interacting region)，一个KIR结构域(Keap1-interacting region)，和一个C端的UBA(Ubiquitin-associated)结构域。N端的PB1结构域负责p62寡聚化以及与其它含PB1结构域的蛋白相互作用。UBA结构域对于寡聚化的p62形成蛋白聚集体是必须的，特别是当细胞暴露于氧化环境中，其作为信号组织中心负责招募泛素化修饰的蛋白底物。作为支架蛋白，p62通过其UBA结构域与蛋白聚集体(protein aggregate)相结合并将其导向自噬体从而将其降解。LIR结构域含有一个富含酸性氨基酸和疏水氨基酸的簇(DDD和WxxL)，该酸性簇和LIR结构域的其它两个关键氨基酸Trp338/Leu341可与LC3蛋白中的多个位点 (N端的碱性氨基酸以及LC3的ubiquitin fold表面的两个疏水口袋)相互作用。LIR结构域与Atg8/LC3的相互作用负责引导泛素化的蛋白到蛋白酶体(proteasome)或自噬体中降解。p62与LC3的荧光共定位和在免疫共沉淀实验过程中被一起沉淀(pull down)，说明它们参与了自噬过程中同一蛋白复合物的形成。在自噬起始的时候，p62与蛋白聚集体的复合物与LC3在自噬体的表面相结合，进而起始了蛋白聚集体的降解过程。p62与LC3蛋白的相互作用对于p62自身的自噬降解也是必须的，抑制自噬将导致p62的大量堆积，然后形成p62和泛素染色阳性的聚集体。Caspase-8被报道与p62相互作用，从而起始非死亡受体依赖的细胞凋亡信号通路。p62的功能异常与人类的多种疾病紧密相关，在肝功能紊乱，肿瘤和多种神经退行性疾病的病人样品中发现了异常堆积的p62聚集体。p62是蛋白聚集物(protein aggregates)中的常见组分，例如帕金森氏病(Parkinson disease)中的Lewy body，艾滋海默病的神经纤维缠节(neurofibrillary tangles)，Huntingtin disease病人的huntingtin aggregates中均含有p62。在佩吉特氏骨病(Paget's disease of bone)，肌萎缩性脊髓侧索硬化症 (amyotrophic lateral sclerosis)以及额颞叶变性(frontotemporal lobar degeneration)等疾病中都有p62的突变被报道。自噬(Autophagy)是一种在进化上高度保守的通过溶酶体吞噬并降解部分自身组分的细胞内分解代谢途径。自噬与多种生理功能有关，在饥饿等不利的环境条件下，细胞通过自噬降解多余或异常的细胞内组分，为细胞的生存提供能量及原材料，促进生物体的生长发育、细胞分化及对环境变化产生应答。自噬异常与多种病理过程如肿瘤、神经退行性疾病、代谢疾病、病原体感染等都有密切关系。由于细胞自噬在生理和病理过程中都有重要作用，自噬已经成为细胞生物学领域的一个新的研究热点。Ad-mCherry-p62是阿拉丁自行研发的重组腺病毒，感染后能够在靶细胞中有效表达红色荧光蛋白mCherry和p62的融合蛋白，呈现明亮的红色荧光，可以用于细胞自噬的检测。在用Ad-mCherry-p62腺病毒感染细胞后，荧光显微镜下mCherry-p62通常以红色点状 (puncta)或较为弥散的斑块状(speckles)荧光存在于细胞质和细胞核中。在自噬被抑制的情况下，这种红色的点状或斑块状物其大小会变大而且数目会变多。p62的荧光标记对于自噬的诱导、自噬的抑制以及蛋白聚集体的清除都是一个非常好的研究工具。图1. Ad-mCherry-p62感染NIH3T3细胞48小时后进行Hoechst 33342染色的效果图。图中可见清晰的点状或斑块状分布的p62的红色荧光。腺病毒感染细胞后为瞬时表达，通常不会与基因组DNA重组，不能用于筛选稳定细胞株。本产品感染体内外细胞后，有效表达时间通常不少于7天，不同细胞和组织的有效表达时间有所不同。感染10-14天后融合蛋白的表达很可能会大大减弱。阿拉丁的Ad-mCherry-p62采用了成熟的E1缺陷型重组腺病毒载体系统，感染普通的细胞后不能进行扩增和重组，从而可以有效降低本产品在活体生物中的风险。本产品可以在表达E1的HEK293A、HEK293等适当细胞中扩增。本产品的滴度≥1×108 pfu/ml，使用时可以按照108 pfu/ml进行计算。对于1ml和10ml包装，如果按照20 MOI感染6孔板的细胞，每孔50万细胞计算，共可以感染10或100个孔；如果按照20 MOI感染24孔板的细胞，每孔10万细胞计算，共可以感染50或500个孔。如果MOI值提高，则相应可以感染的孔数会减少；如果MOI值降低，则相应可以感染的孔数会增加。注意事项：
反复冻融会降低病毒滴度，如有必要请在收到本产品后分装保存。病毒融解后，如果在一周内使用，可以放置于4℃。如果-80℃保存时间超过一年，可能会导致滴度下降，此时建议重新测定病毒滴度。腺病毒感染需要细胞表面的CAR受体，请确认拟使用的细胞或组织可以被腺病毒感染。本产品使用前请仔细阅读附录1《腺病毒使用安全规范》。本产品生物安全等级为Biosafety Level 1 (BSL-1)，没有确凿证据显示会导致健康成人产生疾病(Not known to consistently cause diseases in healthy adults)，可以按照常规的微生物实验操作要求进行操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。使用说明：
1. 感染条件的确定：不同种类的细胞感染所需的MOI值是不同的，如果是初次使用腺病毒感染需要通过实验确定最佳的感染条件。a.细胞培养(以6孔板NIH3T3细胞为例，其它培养板或培养皿参考6孔板进行操作)：感染前一天在6孔板中以5×105/孔接种NIH3T3细胞，每孔加入2ml完全培养液(具体的接种数量由细胞大小和细胞生长速度而定)，使第二天病毒感染时细胞密度达到约50%左右。b.按照MOI 分别为2、5、10、20、40，计算所需病毒量，计算方法请参考附录2。c.冰上解冻病毒，混匀后备用。d.从细胞培养箱中取出6孔板，在显微镜下确定每孔的细胞均生长良好。吸弃旧培养液，每孔加入1.2ml新鲜培养液，并分别加入特定MOI值的病毒液，同时设置未加入病毒的细胞孔作为对照组。须特别注意在病毒感染时加入尽量少的新鲜培养液，以提升病毒的感染效率。对于培养液体积比较小的多孔板，例如96孔板和48孔板，也可以先把病毒用培养液稀释至所需的MOI值后再加入。e.感染后约24小时，除去含有病毒的培养液，每孔加入2ml新鲜的完全培养液，继续培养24小时后观察细胞生长状况及荧光蛋白表达情况(参考图2)，以不显著影响细胞生长、荧光较强且感染效率便于荧光观察的MOI值和感染后时间为最佳条件。注1：检测细胞自噬时并非感染效率越高越好，通常约20-70%的感染效率已经足以进行自噬检测，感染效率过高反而容易产生细胞毒性而干扰检测。注2：通常在腺病毒感染细胞后24小时即可观察到荧光蛋白表达的出现，在48小时左右有较强的表达效果。注3：对于感染腺病毒后出现较强细胞毒性的情况，可以尝试在感染后6-12小时更换成新鲜的完全培养液。 图2. Ad-mCherry-p62感染细胞的效果图。A、B、C、D、E和F依次为Ad-mCherry-p62分别在MOI值为0、2、5、10、20和40时感染NIH3T3细胞48小时后的实拍效果图。上侧为明场照片，下侧为相同视野的荧光照片。2.感染细胞、抑制自噬和荧光观察按照步骤1获得的条件进行感染实验。在不诱导自噬或使用EBSS等诱导自噬的情况下，使用chloroquine或bafilomycin A1等细胞自噬抑制剂抑制自噬，随后可在荧光显微镜下观察p62的荧光变化情况。附录：1.腺病毒使用安全规范a.作为一种相对安全的病毒，尽管腺病毒基因组在感染后不会整合进入宿主细胞基因组中，也不会在细胞体内进行复制，但是仍然具有可能的潜在生物学危险。我们建议使用者在病毒操作前应仔细阅读本规范，并在实验中严格按照本规范的要求进行操作。更为严格的美国CDC的生物安全等级及其操作与防护要求参考附表1，也可以访问如下网页：https://www.cdc.gov/labs/pdf/CDC-BiosafetyMicrobiologicalBiomedicalLaboratories-2020-P.pdf。b.腺病毒操作时应使用相应级别的生物安全柜，不同的腺病毒的生物安全等级会有所不同。如果使用普通超净工作台操作病毒，请不要打开排风机，以尽量避免可能污染病毒的尘埃正面吹向操作人员而被吸入。c.实验操作时必需佩戴一次性帽子、口罩，穿戴实验手套及专门的实验服，避免身体直接接触病毒。手部及面部有开放性创口时，禁止进行病毒操作。d.操作病毒时需小心谨慎，不要产生气雾或飞溅。如果操作时超净工作台或其它器皿上有病毒污染，请立即用70%乙醇或2%SDS溶液擦拭干净，或者采取其它的妥善措施。e.如果需要离心，应使用密封性好的离心管，或用封口膜密封后离心，最好使用专门的离心机。f.用显微镜观察细胞感染情况时应遵从以下步骤：拧紧培养瓶或盖紧培养板，用70%乙醇清理培养瓶或培养板外壁后到显微镜处观察拍照。离开显微镜实验台之前，用70%乙醇擦洗显微镜实验台。g.所有被病毒污染过的枪头、离心管、培养板(皿、瓶)、培养液、手套等，在丢弃前请用84消毒液或2%SDS浸泡过夜。h.脱掉手套后，用肥皂或洗手液清洗双手。2.病毒MOI的计算MOI (Multiplicity of Infection)定义：病毒感染细胞时，病毒数量与细胞数量的比值。Pfu (Plaque forming units)定义：具有生物活性的病毒颗粒数量。可以根据如下公式计算所需pfu：所需pfu=细胞数量×MOI例如，需要向1×105细胞中加入2 MOI的病毒，即所需pfu=(1×105 cells)×(2 MOI)=2×105 pfu。若病毒母液滴度为1×108pfu/ml，则细胞培养液中应加入(2×105 pfu)/(1×108 pfu/ml)=0.002ml病毒母液，即2μl病毒母液。附表1. 生物安全等级及其操作与防护要求Table 1. Summary of Recommended Biosafety Levels for Infectious Agents BSL Agents Practices Primary Barriers andSafety Equipment Facilities(Secondary Barriers) 1 Not known to consistently cause diseases in healthy adults Standard microbiological practices ■ No primary barriers required.■ PPE: laboratory coats and gloves; eye, face protection, as needed Laboratory bench and sink required 2 ■ Agents associated with human disease■ Routes of transmission include percutaneous injury, ingestion, mucous membrane exposure BSL-1 practice plus: ■ Limited access■ Biohazard warning signs■ “Sharps” precautions■ Biosafety manual defining any needed waste decontamination or medical surveillance policies Primary barriers:■ BSCs or other physical containment devices used for all manipulations of agents that cause splashes or aerosols of infectious materials■ PPE: Laboratory coats, gloves, face and eye protection, as needed BSL-1 plus:■ Autoclave available 3 Indigenous or exotic agents that may cause serious or potentially lethal disease through the inhalation route of exposure BSL-2 practice plus: ■ Controlled access■ Decontamination of all waste■ Decontamination of laboratory clothing before laundering Primary barriers:■ BSCs or other physical containment devices used for all open manipulations of agents■ PPE: Protective laboratory clothing, gloves, face, eye and respiratory protection, as needed BSL-2 plus:■ Physical separation from access corridors■ Self-closing, double-door access■ Exhausted air not recirculated■ Negative airflow into laboratory■ Entry through airlock or anteroom■ Hand washing sink near laboratory exit 4 ■ Dangerous/exotic agents which post high individual risk of aerosol-transmitted laboratory infections that are frequently fatal, for which there are no vaccines or treatments■ Agents with a close or identical antigenic relationship to an agent requiring BSL-4 until data are available to redesignate the level■ Related agents with unknown risk of transmission BSL-3 practices plus:■ Clothing change before entering■ Shower on exit■ All material decontaminated on exit from facility Primary barriers:■ All procedures conducted in Class III BSCs or Class I or II BSCs in combination with full-body, air-supplied, positive pressure suit BSL-3 plus:■ Separate building or isolated zone■ Dedicated supply and exhaust, vacuum, and decontamination systems■ Other requirements outlined in the text BSL, biosafety level; PPE, personal protective equipment.Ad-mCherry-p62 (adenovirus expressing mCherry-p62 fusion protein) is a recombinant adenovirus developed by aladdin, which can express red fluorescent mCherry-p62 fusion protein high efficiently in target cells or tissues after infection. It is widely used for the detection of autophagy in target cells or tissues exhibiting the bright red fluorescence.Ad-mCherry-p62 does not recombine with genomic DNA of target cells or tissues, and the expression of mCherry-P62 fusion protein is transient. Therefore, it cannot be used to screen stable cell lines. After infection of cells, the expression of mCherry-P62 can last for at least 7 days usually and decreases significantly after 10-14 days, which may vary depending on the type of cells and tissues.Beotime's Ad-mCherry-p62 employs a mature E1-deficient recombinant adenovirus vector system which can only amplify in E1 expressing cell lines such as HEK293A and HEK293, but not in ordinary cells, thus effectively reducing the risks of this product.p62, also known as sequestosome I (SQSTM1), is highly conserved in multicellular organisms (excluding plants and fungi). It is predominantly distributed in cytoplasm, but also present in nuclei, autophagosomes, and lysosomes. p62, a stress-induced protein, plays an important role as a signaling hub in many cellular events such as amino acid sensing and oxidative stress, and also serves as a scaffold protein for PKC, ERK1, mTORC1, NF-кB, and caspase-8, among others. p62 is 440 amino acids long, with multiple domains such as a PB1 domain, a zinc finger domain, two nuclear localization signals, a TRAF6 binding domain, and a LIR domain. The N-terminal PB1 domain is required for oligomerization of p62 to form protein aggregates especially when cells are exposed to oxidative environments, and acts as a signaling organization center responsible for recruiting ubiquitination-modified protein substrates. The LIR domain contains a cluster rich in acidic and hydrophobic amino acids (DDD and WxxL). The acidic cluster and the other two key amino acids of the LIR domain, Trp338/Leu341, interact with LC3 protein at multiple sites, which is responsible for directing the degradation of ubiquitinated proteins into the proteasome or autophagosome. The interaction between p62 and LC3 is also necessary for the autophagic degradation of p62 itself, and inhibition of autophagy will lead to a large accumulation of p62, followed by the formation of p62 and ubiquitin staining-positive aggregates. Caspase-8 is reported to interact with p62 to initiate non-death receptor-dependent apoptotic signaling pathways.Autophagy is a highly evolutionarily conserved intracellular metabolic pathway for partial degradation of its own components through lysosomal phagocytosis, which is associated with a variety of physiological functions. Under unfavorable environmental conditions such as starvation, cells degrade excess or abnormal intracellular components through autophagy to provide energy and raw materials for cell survival and to promote growth and development, cell differentiation, and response to environmental changes. Abnormalities in autophagy are closely related to a variety of pathological processes such as tumors, neurodegenerative diseases, metabolic diseases, and pathogenic infections. Since cellular autophagy plays important roles in both physiological and pathological processes, it has become a new research topic in the field of cell biology.After infection of cells with the Ad-mCherry-p62, mCherry-p62 usually fluoresces as red puncta or diffusive speckles in cytoplasm and nucleus under fluorescence microscopy. When autophage is inhibited, the red punctas or speckles become larger and more numerous. The fluorescent labeling of p62 is an excellent tool for studying autophagy induction and inhibition, and the clearance of protein aggregates as well.Figure 1. NIH3T3 cells infected with the Ad-mCherry-p62 were stained by Hoechst 33342 after 48 hours post-infection. The red punctas or speckles of mCherry-p62 is visible clearly.The titer of this product is ≥1×108 pfu/ml. If cells are infected at 20 MOI, 1ml of this product is sufficient for infecting 10 wells with 500,000 cells/well in 6-well plates, or 50 wells with 100,000 cells/well in 24-well plates. The actual number of wells that can be infected is also dependent on the MOI value used.
Precautions：
PrecautionsPrecautions for Adenovirus Use" carefully. This product has a Biosafety Level 1 (BSL-1). It is not known to consistently cause diseases in healthy adults and can be used in accordance with standard microbiological practices.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves while handling the reagent.Instructions for Use：
1. Determination of the infection conditionsThe following protocol is provided for Ad-mCherry-p62 infection of NIH3T3 cells in 6-well plates. Make appropriately adjustments for the infection of cells cultured in other types of vessels. Different MOI values are required for different types of cells. For first-time users of this product, please optimize the infection conditions. a. Culture NIH3T3 cells in 6-well cell culture plates: The day before infection, seed NIH3T3 cells at 5×105 cells/well in 2ml of complete culture medium. Cells density should be approximately 50% for virus infection. Note: The exact number of cells inoculated is dependent on the cell size and cell growth speed to achieve a cell density of 50% approximately for virus infection next day.b. Calculate the amount of virus required to yield a MOI value of 2, 5, 10, 20 and 40, respectively. Please refer to Appendix 2 for the calculation.c. Thaw the virus on ice and mix well before use.d. Take out the 6-well plate from the cell culture incubator and examine cells by microscopy to make sure cells are in good growth. Replace the old culture medium with 1.2ml of fresh culture medium per well, and add an appropriate amount of Ad-mCherry-p62 to yield a specific MOI value per well. At the meantime, set up wells without virus as negative control. Note: Fresh culture medium should be added to each well as little as possible to increase the efficiency of virus infection. For multi-well plates with a small volume of culture medium, such as 96-well and 48-well plates, the Ad-mCherry-p62 can also be diluted with culture medium to the desired MOI value before addition.e. After 24 hours post-infection, remove the culture medium containing virus. Add 2ml of fresh complete culture medium per well, and continue to incubate for 24 hours before examination by a fluorescence microscopy (Figure 2). The optimal MOI value is the one that provides strong fluorescence with high infection efficiency and has no significant influence on cell growth.Note 1: Higher infection efficiency is not necessarily the better, because too high infection efficiency may easily cause cytotoxicity and interfere with the detection of autophagy. An optimal infection efficiency is usually 20-70%.Note 2: The expression of fluorescent mCherry-p62 can be detected usually 24 hours post-infection, and peaks after 48 hours approximately.Note 3: In cases of high cytotoxicity after adenovirus infection, replacing with fresh complete culture medium 6-12 hours after infection can be attempted.Figure 2. NIH3T3 cells infected with Ad-mCherry-p62 (Beytome, #C3016) at different MOI values as indicated in the figure. Cells were examined 48 hours after infection. mCherry-p62 has an excitation maximum at 587nm and an emission maximum at 610nm.2. Cell infection, autophagy inhibition and fluorescence observationa. Perform infection with Ad-mCherry-p62 at the optimal MOI value determined in step 1. b. Induce autophagy with EBSS (Beytome, #C0213/C0214). Cells without EBSS treatment are used as negative control.c. Inhibit autophagy with autophagy inhibitors such as chloroquine or bafilomycin A1.d. Examine the changes of red fluorescent mCherry-p62 by fluorescence microscopy.Appendix：1. Biosafety practices for use of adenovirusa. As a relatively safe virus, the adenovirus genome does not integrate into the host genome or replicate inside cell after infection, but its potential biological risks can not be excluded. We recommend users reading this specification carefully before handing virus and following it strictly during the operation. For more information about the US CDC biosafety levels and their handling and protection requirements, please visit the website at:http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5_sect_IV.pdf.b. Use appropriate biosafety cabinets when handling the adenovirus, which may vary for different adenoviruses. If a laminar flow hood is used, please turn off the blower fan to prevent the contaminated dust with virus from being inhaled. c. Please wear disposable hat, mask, gloves and lab coat to avoid direct contact with the adenovirus. Do not handle the virus when there are wounds in open part your body.d. When handling the virus, be careful not to produce aerosol or splash. If there is virus contamination in the laminar flow hood or on other utensils, wipe it off immediately with 70% ethanol or 2% SDS solution, or take other appropriate measures.e. If centrifugation is required, seal the centrifuge tube tightly before centrifuge. A dedicated centrifuge for virus operation is preferred.f. To examine cells after infection by fluorescence microscope, the following steps should be followed: tighten the culture bottle or plate, clean the outer surface with 70% ethanol, and then examine cells by microscopy. After examination, clean the microscope stage with 70% ethanol.g. All virus contaminated tips, centrifuge tubes, culture vessels, cultures, gloves should be sterilized overnight in disinfectant or 2% SDS solution before discarding.h. Wash hands with soap or sanitizer after removing gloves.2. Calculation of viral MOIMOI (Multiplicity of Infection): A ratio of the number of viruses to the number of cells to be infected.Pfu (Plaque forming units): The number of biologically active virus particles.pfu can be calculated according to the following formula:pfu required = number of cells x MOIFor example, to infect 1×105 cells with a virus at a MOI value of 2, the required pfu is 2×105. If the titer of viral stock is 1×108 pfu/ml, then (2×105 pfu)/(1×108 pfu/ml) = 0.002ml of viral stock should be used.3. Table 1. Summary of Recommended Biosafety Levels for Infectious AgentsBSLAgentsPracticesPrimary Barriers and Safety EquipmentFacilities (Secondary Barriers)1Not known to consistently cause diseases in healthy adultsStandard microbiological practices■ No primary barriers required.■ PPE: laboratory coats and gloves; eye, face protection, as neededLaboratory bench and sink required2■ Agents associated with human disease■ Routes of transmission include percutaneous injury, ingestion, mucous membrane exposureBSL-1 practice plus:■ Limited access■ Biohazard warning signs■ “Sharps” precautions■ Biosafety manual defining any needed waste decontamination or medical surveillance policiesPrimary barriers:■ BSCs or other physical containment devices used for all manipulations of agents that cause splashes or aerosols of infectious materials■ PPE: Laboratory coats, gloves, face and eye protection, as neededBSL-1 plus:■ Autoclave available3Indigenous or exotic agents that may cause serious or potentially lethal disease through the inhalation route of exposureBSL-2 practice plus:■ Controlled access■ Decontamination of all waste■ Decontamination of laboratory clothing before launderingPrimary barriers:■ BSCs or other physical containment devices used for all open manipulations of agents■ PPE: Protective laboratory clothing, gloves, face, eye and respiratory protection, as neededBSL-2 plus:■ Physical separation from access corridors■ Self-closing, double-door access■ Exhausted air not recirculated■ Negative airflow into laboratory■ Entry through airlock or anteroom■ Hand washing sink near laboratory exit4■ Dangerous/exotic agents which post high individual risk of aerosol-transmitted laboratory infections that are frequently fatal, for which there are no vaccines or treatments■ Agents with a close or identical antigenic relationship to an agent requiring BSL-4 until data are available to redesignate the level■ Related agents with unknown risk of transmissionBSL-3 practices plus:■ Clothing change before entering■ Shower on exit■ All material decontaminated on exit from facilityPrimary barriers:■ All procedures conducted in Class III BSCs or Class I or II BSCs in combination with full-body, air-supplied, positive pressure suitBSL-3 plus:■ Separate building or isolated zone■ Dedicated supply and exhaust, vacuum, and decontamination systems■ Other requirements outlined in the textBSL, biosafety level; PPE, personal protective equipment.规格
产品名称
Ad-mCherry-p62
别名
腺病毒载体
分子类型
蛋白质
产品线
生物产品
储存与运输
储存条件
-80℃储存
运输条件
特低温运输
稳定性与储存
-80℃保存，一年有效。-20℃保存，1-2个月内有效。4℃保存，一周内有效。
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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