人血清白蛋白溶液可作为RPMI 1640培养基的补充剂,用于多形核细胞(PMN)的培养。也可作为培养基,用于单核细胞来源树突状细胞的分化和成熟。应用:
人血清白蛋白(HSA)是人血浆蛋白的主要成分,为单体形式的α螺旋球蛋白。多肽单链由585个氨基酸残基组成,链内含有17个二硫键桥和1个游离半胱氨酸。人血清白蛋白经过三种不同的翻译后修饰:氧化、糖基化和S-亚硝基化。修饰通常发生在球状蛋白的表面,不显著影响构象。然而,修饰强烈影响脂肪酸和药物分子的结合。
Human serum albumin (HSA) is a monomeric, globular, and α-helical protein that constitutes a major part of human blood plasma proteins. This single-chain polypeptide protein contains 585 amino acid residues and 17 internal disulfide bridges and one free cysteine.
Human serum albumin undergoes three different post-translational modifications: oxidation, glycation, and S-nitrosylation. Modifications usually occur on the surface of the globular protein, and do not significantly affect conformation. However, modification strongly affects binding of fatty acids and drug molecules.
Application:
Albumin solution human has been used as a supplement in RPMI 1640 media during polymorphonuclear cell (PMN) incubation. It has also been used as a culture media for the differentiation and maturation of monocyte-derived dendritic cells.