Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
宿主种属
兔(Rabbit)
特异性
Caspase3
种属反应性
人(Human)
免疫原
A synthetic peptide derived from human Caspase 3 (AA 52-120).
阳性对照
WB: THP-1, Jurkat, Raji, HEK293 and K562 cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa cells. IP: HeLa cells.
Recombinant Caspase3 Antibody (Ab093009) - Western Blot All lanes: Recombinant Caspase3 Antibody (Ab093009) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 32 kDa Observed band size: 30 kDa Exposure time: 12.3 s
Recombinant Caspase3 Antibody (Ab093009) - Western Blot All lanes use Recombinant Caspase3 Antibody (Ab093009) at 1/1000 dilution for 1 hour at room temperature.
Recombinant Caspase3 Antibody (Ab093009) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Caspase3 Antibody (Ab093009).
Recombinant Caspase3 Antibody (Ab093009) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Caspase3 Antibody (Ab093009).
Recombinant Caspase3 Antibody (Ab093009) - IP Immunoprecipitate (IP) analysis using Recombinant Caspase3 Antibody (Ab093009) at 1/50 dilution. (WB with Recombinant Caspase3 Antibody (Ab093009) at 1/2000 dilution)
ZJ25F0623279
危险品化学品经营许可证（带存储）