Mouse anti GFP Antibody, Monoclonal antibody(F56-6A1.2.3), could be used for WB, ICC, IF and so on.
规格
产品名称
GFP Mouse mAb
别名
GFP tag 抗体 | GFP tag 单克隆抗体 | GFP tag 标签单克隆抗体 | GFP小鼠单克隆抗体 | GFP小鼠单抗
英文别名
GFP antibody | Green fluorescent protein antibody | eGFP | Enhanced Green Fluorescent Protein | GFP | GFPuv | green fluorescent protein (gfp) | Green Fluorescent Protein | GFP Tag antibody | Anti- GFP Tag antibody
规格或纯度
无载体, 无叠氮钠, 已验证, PBS Only, ≥95%(SDS-PAGE), 见COA
生化机理
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca 2+ -activated photoprotein aequorin. Subunit structure: Monomer. Tissue specificity: Photocytes. Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. Sequence similarities: Belongs to the GFP family. Biophysicochemical properties: Absorption: Abs(max)=395 nm Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
宿主种属
小鼠(Mouse)
特异性
GFP
种属反应性
不依赖种属(Species independent)
免疫原
Full length enhanced GFP.
阳性对照
HEK293 cells transfected with GFP tag.
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
F56-6A1.2.3
Format
Whole IgG
亚型
IgG2b
纯化方法
Protein G purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, pH 7.0
防腐剂
No
储存条件
2-8°C储存,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
4°C 下运输。4°C 长期保存（12 个月）。请勿冷冻。收货后建议分装。
图片
GFP Mouse mAb (Ab105280) - Western Blot All lanes: GFP Mouse mAb (Ab105280) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2.8 s
ZJ26F0130911
危险品化学品经营许可证（带存储）