mCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric, fluorescent, photostable red dye that was isolated from and further engineered from the Discosoma sea anemone. Its peak fluorescent excitation is at 587 nm and emission 610 nm.
宿主种属
小鼠(Mouse)
特异性
mCherry
种属反应性
不依赖种属(Species independent)
免疫原
Recombinant full length protein corresponding to mCherry
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
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mCherry Mouse mAb (Ab169012) - Western Blot All lanes: mCherry Mouse mAb (Ab169012) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 45, 55 kDa Observed band size: 47, 55 kDa Exposure time: 60.0 s
mCherry Mouse mAb (Ab169012) - Flow Cytometry Overlay histogram showing HEK293T cells transfected with a mCherry tag using mCherry Mouse mAb (Ab169012) (Right panel). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (AF647) (Ab181952) was used as the secondary antibody. The mouse IgG (Ab170221) was used as an isotype control (Left panel).
mCherry Mouse mAb (Ab169012) – IF/ICC IF analysis of 293T cells transfected with a mCherry-DYKDDDDK tag using mCherry Mouse mAb (Red). The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with mCherry Mouse mAb (Ab169012) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (AF647) (Ab181952) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.