Goat Anti-Chicken IgY H&L Antibody, Secondary antibody, could be used for WB, ELISA, CLIA, LF, GICA and so on. WB: 1/400 - 1/2000 ELISA : 1/5000 - 1/15000 LF: 0.5-1.0mg/ml
No cross reactivity with mouse IgG, rabbit IgG, and human IgG.
免疫原
Chicken IgY
偶联
Unconjugated
抗体类型
secondary antibody
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Goat IgG
纯化方法
Antigen affinity purified
来源
Serum
浓度
6.0 mg/ml
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, pH 7.4
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
Goat Anti-Chicken IgY H&L Antibody (Ab170784) - ELISA Goat Anti-Chicken IgY H&L Antibody (Ab170784) was tested by direct ELISA. The plate was coated with different amounts of Chicken IgY Antibody (Ab008417). A serial dilution Goat Anti-Chicken IgY H&L Antibody (Ab170784) was then added at a series of different dilution ratios. Rabbit Anti-Goat IgG H&L (HRP) (Ab141534) was used at 1/10000 dilution as the secondary antibody.
Goat Anti-Chicken IgY H&L Antibody (Ab170784) - ELISA Cross-reactivity of Goat Anti-Chicken IgY H&L Antibody (Ab170784) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Chicken IgY H&L Antibody was then added at different dilution ratio followed by incubation for 1h. Rabbit Anti-Goat IgG H&L (HRP) (Ab141534) was used at 1/10000 dilution as the secondary antibody.
Goat Anti-Chicken IgY H&L Antibody (Ab170784) - SDS-PAGE 3 μg/lane of Goat Anti-Chicken IgY H&L Antibody (Ab170784) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing the band at 26.1 kDa (Light Chain) & 53.1 kDa (Heavy Chain), under reducing conditions; 162.9 kDa, under non-reducing conditions.