Rabbit Anti-Chicken IgY H&L Antibody, Secondary antibody, could be used for WB, CLIA, LF, GICA, EIA and so on. WB: 1/2000 - 1/10000 CLIA: 0.5µg/mL-10µg/mL LF/ GICA: 0.5mg/mL-2.0mg/mL EIA: 1/1000 - 1/50000
Rabbit Anti-Chicken IgY H&L Antibody (Ab176136) - ELISA Rabbit Anti-Chicken IgY H&L Antibody (Ab176136) was tested by ELISA. The plate was coated with 0.5 μg/mL Chicken IgY (Ab008417). Rabbit Anti-Chicken IgY H&L Antibody was biotinylated then added at a series of different dilution ratios. Streptavidin-HRP (np156148) was used at 1/40000 dilution for this ELISA.
Rabbit Anti-Chicken IgY H&L Antibody (Ab176136) - ELISA Cross-reactivity of Rabbit Anti-Chicken IgY H&L Antibody (Ab176136) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Rabbit Anti-Chicken IgY H&L Antibody was biotinylated then added at different dilution ratio followed by incubation for 1h. Streptavidin-HRP (np156148) was used at 1/40000 dilution for this ELISA.