Anti-mouse CD25 (IL-2Rα)-InVivo | In Vivo MAb anti-mouse CD25 (IL-2Rα) | CD25 Rat mAb | CD25, Il2r, Ly-43 | Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2
规格或纯度
ExactAb™, 已验证, 无叠氮钠, Ex:498nm, Em:517nm, 5μL/test
宿主种属
大鼠(Rat)
特异性
CD25
种属反应性
小鼠(Mouse)
免疫原
IL-2-dependent cytolytic mouse T cell clone B6.1
阳性对照
Flow: Murine splenocytes
偶联
FITC
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
517nm
Ex/Em(nm)
Ex:498nm;Em:517nm
克隆类型
单克隆抗体
克隆号
PC-61.5.3
Format
Whole IgG
亚型
IgG1
轻链亚型
lambda
纯化方法
Protein A High Salt
浓度
5μL/test
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
未稀释的抗体溶液应保存在 2°C 至 8°C 之间,避免长时间暴露在光线下。切勿冷冻。
图片
CD25 Rat mAb (FITC) (Ab179009) - Flow Cytometry BALB/c mouse splenocytes are stained with CD25 Rat mAb (FITC) (Ab179009) and CD4 Rat mAb (APC) (Ab181112) (right). BALB/c mouse splenocytes are stained with CD4 Rat mAb (APC) (Ab181112) and Rat IgG Isotype Control Antibody (FITC) (Ab180311) (left).
CD25 Rat mAb (FITC) (Ab179009) - Flow Cytometry Con A-stimulated (1 day) BALB/c mouse splenocytes (right panel) stained with CD25 Rat mAb (FITC) (Ab179009) (red) compared to an isotype control (blue). Blue - Rat IgG Isotype Control Antibody (FITC) (Ab180311). Black - Unlabelled control, cells without incubation with primary antibody. Untreated BALB/c mouse splenocytes were used as control (left panel).
应用
应用名称
稀释比例
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.