Rat anti Mouse CD25 Antibody, Monoclonal (PC-61.5.3), could be used for Flow Cytometry
规格
产品名称
CD25 Rat mAb (AF488)
别名
CD25抗体
英文别名
Anti-mouse CD25 (IL-2Rα)-InVivo | In Vivo MAb anti-mouse CD25 (IL-2Rα) | CD25 Rat mAb | CD25, Il2r, Ly-43 | Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
宿主种属
大鼠(Rat)
特异性
CD25
种属反应性
小鼠(Mouse)
免疫原
IL-2-dependent cytolytic mouse T cell clone B6.1
阳性对照
Flow: C57BL/6 mouse splenocytes
偶联
AF488
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
lambda
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
CD25 Rat mAb (AF488) (Ab211656) - Flow Cytometry Con A-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) were stained with CD25 Rat mAb (AF488) (Ab211656) (red) compared to an isotype control (blue). Blue - Rat IgG (AF488) (Ab190240). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).
CD25 Rat mAb (AF488) (Ab211656) - Flow Cytometry Con A-stimulated (3 days) C57BL/6 mouse splenocytes were stained with CD25 Rat mAb (AF488) (Ab211656) (right panel) compared to an isotype control (left panel). Left panel - Rat IgG (AF488) (Ab190240).
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.