Recombinant fragment corresponding to Human IL2 Receptor beta/p75 aa 1-250. Expressed in E.coli.
阳性对照
Flow: C57BL/6 mouse splenocytes.
偶联
Biotin
克隆类型
单克隆抗体
克隆号
TM-β1
Format
Whole IgG
亚型
Rat IgG2b
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
未稀释的抗体溶液应保存在 2°C 至 8°C 之间,避免长时间暴露在光线下。切勿冷冻。
图片
CD122/IL-2Rβ Rat mAb (Biotin) (Ab185171) - Flow Cytometry Flow cytometry analysis of CD122/IL-2Rβ expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD122/IL-2Rβ Rat mAb (Biotin) (Ab185171) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).
CD122/IL-2Rβ Rat mAb (Biotin) (Ab185171) - Flow Cytometry Flow cytometry analysis of CD122/IL-2Rβ expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD122/IL-2Rβ Rat mAb (Biotin) (Ab185171) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤0.5μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.