Rat anti Mouse CD122/IL-2Rβ Antibody, Monoclonal (TM-β1), could be used for Flow Cytometry.
规格
产品名称
CD122/IL-2Rβ Rat mAb (PE)
别名
白介素2受体β链(CD122)抗体 | CD122抗体 | Anti-CD122抗体
英文别名
CD122 antigen | CD122 | high affinity IL-2 receptor beta subunit | High affinity IL-2 receptor subunit beta | IL-15 R beta | IL-2 R beta | IL-2 receptor subunit beta | IL2R beta | IL-2R subunit beta | IL2RB | IL-2Rb | interleukin 15 receptor, beta | inter
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
生化机理
Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
宿主种属
大鼠(Rat)
特异性
CD122/IL-2Rβ
种属反应性
小鼠(Mouse)
免疫原
Recombinant fragment corresponding to Human IL2 Receptor beta/p75 aa 1-250. Expressed in E.coli.
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes.
偶联
PE
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
CD122/IL-2Rβ Rat mAb (PE) (Ab185173) - Flow Cytometry Flow cytometry analysis of CD122/IL-2Rβ expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD122/IL-2Rβ Rat mAb (PE) (Ab185173) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).
CD122/IL-2Rβ Rat mAb (PE) (Ab185173) - Flow Cytometry Flow cytometry analysis of CD122/IL-2Rβ expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD122/IL-2Rβ Rat mAb (PE) (Ab185173) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.