Application: Flow: ≤0.3 μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.
Transfected k562 cells expressing the extracellular domain of murine CD19.
阳性对照
Flow: Mouse splenocytes.
偶联
APC
Excitation(nm)
Red Laser(633nm)
Emission(nm)
660nm
Ex/Em(nm)
Ex:650nm;Em:660nm
克隆类型
单克隆抗体
克隆号
1D3
Format
Whole IgG
亚型
Rat IgG2a
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
未稀释的抗体溶液应保存在 2°C 至 8°C 之间,避免长时间暴露在光线下。切勿冷冻。
图片
CD19 Rat mAb (APC) (Ab185395) - Flow Cytometry Flow cytometry analysis of CD19 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD19 Rat mAb (APC) (Ab185395) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG Isotype Control Antibody (APC) (Ab180313).
CD19 Rat mAb (APC) (Ab185395) - Flow Cytometry Flow cytometry analysis of CD19 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD19 Rat mAb (APC) (Ab185395) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (APC) (Ab180313). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤0.3μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.