Recombinant CD19 Antibody, clone P99G09, could be used for Flow Cytometry.
规格
产品名称
Recombinant CD19 Antibody (Biotin)
别名
白细胞分化抗原19|CD19抗体
英文别名
Antibody deficiency due to defect in CD19 antibody|Antibody deficiency due to defect in CD19, included antibody|AW495831 antibody|B lymphocyte antigen CD19 antibody|B lymphocyte surface antigen B4 antibody|B-lymphocyte antigen CD19 antibody|B-lymphocyte surface antigen B4 antibody|B4 antibody|CD19 antibody|CD19 antigen antibody|CD19 molecule antibody|Cd19 protein antibody|CVID3 antibody|Differentiation antigen CD19 antibody|Leu 12 antibody|Leu-12 antibody|Leu12 antibody|MGC109570 antibody|MGC12802 antibody|T-cell surface antigen Leu-12 antibody|B-lymphocyte antigen CD19|B-lymphocyte surface antigen B4|CD19 antigen|CD19 molecule|CD19|Differentiation antigen CD19|Leu-12|T-cell surface antigen Leu-12
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
生化机理
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
宿主种属
大鼠(Rat)
特异性
Mouse CD19
种属反应性
小鼠(Mouse)
免疫原
Mouse CD19-expressing K562 human erythroleukemia cells
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
Biotin
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant CD19 Antibody (Biotin) (Ab213879) - Flow Cytometry Flow cytometry analysis of CD19 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD19 Antibody (Biotin) (Ab213879) at 0.04μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG (Biotin) (Ab180310).
Recombinant CD19 Antibody (Biotin) (Ab213879) - Flow Cytometry Flow cytometry analysis of CD19 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD19 Antibody (Biotin) (Ab213879) 0.04μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤0.3μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.
ZJ25F0218393
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