Rat anti Mouse CD44 Antibody, Monoclonal (IM7), could be used for Flow Cytometry.
规格
产品名称
CD44 Rat mAb (AF647)
别名
归巢关联细胞黏附分子(HCAM)抗体|CD44抗体
英文别名
Ly-24|Pgp-1|HERMES|CD44 antigen|CD44 molecule (Indian blood group)|CD44|CD44R|CDw44|cell surface glycoprotein CD44|chondroitin sulfate proteoglycan 8|CSPG8|ECMR-III|epican|Extracellular matrix receptor III|GP90 lymphocyte homing/adhesion receptor|HCAM|HCELL|hematopoietic cell E- and L-selectin ligand|Heparan sulfate proteoglycan|Hermes antigen|homing function and Indian blood group system|HUTCH-I|Hyaluronate receptor|IN|MC56|MDU2|MDU2CD44 antigen (homing function and Indian blood group system)|MDU3|MDU3CDW44|MIC4|MIC4MGC10468|MUTCH-I|Pgp1|PGP-I|Phagocytic glycoprotein 1|Phagocytic glycoprotein I|LHR antibody|BA-1 antibody|CD 44 antibody|CD44 antibody|CD44 antigen antibody|CD44 molecule (Indian blood group) antibody|CD44 molecule antibody|CDw44 antibody|CDW44 antigen antibody|Cell surface glycoprotein CD44 antibody|chondroitin sulfate proteoglycan 8 antibody|CSPG8 antibody|ECMR-III antibody|Epican antibody|Extracellular matrix receptor III antibody|GP90 lymphocyte homing/adhesion receptor antibody|HCELL antibody|hematopoietic cell E- and L-selectin ligand antibody|Heparan sulfate proteoglycan antibody|Hermes antigen antibody|homing function and Indian blood group system antibody|HSA antibody|HUTCH-I antibody|HUTCH1 antibody|HUTCHI antibody|Hyaluronate receptor antibody|IN antibody|INLU-related p80 Glycoprotein antibody|MC56 antibody|MDU2 antibody|MDU3 antibody|MGC10468 antibody|MIC4 antibody|MUTCH I antibody|MUTCH1 antibody|PGP-1 antibody|PGP-I antibody|PGP1 antibody|Phagocytic glycoprotein 1 antibody|Phagocytic glycoprotein I antibody|Soluble CD44 antibody
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
生化机理
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
宿主种属
大鼠(Rat)
特异性
CD44
种属反应性
小鼠(Mouse)
免疫原
Dexamethasone-induced myeloid leukemia M1 cells
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
AF647
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
CD44 Rat mAb (AF647) (Ab190184) - Flow Cytometry Flow cytometry analysis of CD44 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD44 Rat mAb (AF647) (Ab190184) at 0.04μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF647) (Ab190071).
CD44 Rat mAb (AF647) (Ab190184) - Flow Cytometry Flow cytometry analysis of CD44 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD44 Rat mAb (AF647) (Ab190184) at 0.04μg/test for 1 hour at 4°C. Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤0.3μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.