Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
宿主种属
大鼠(Rat)
特异性
Transferrin Receptor/CD71
种属反应性
小鼠(Mouse)
免疫原
Mouse erythroleukemia cell line 745.6
阳性对照
Flow: C57BL/6 mouse bone marrow cells
偶联
APC
Excitation(nm)
Red Laser(633nm)
Emission(nm)
660nm
Ex/Em(nm)
Ex:650nm;Em:660nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Transferrin Receptor/CD71 Rat mAb (APC) (Ab190204) - Flow Cytometry Flow cytometry analysis of Transferrin Receptor/CD71 expression by C57BL/6 mouse bone marrow cells (right panel) compared to an isotype control (left panel). Cells were stained with Transferrin Receptor/CD71 Rat mAb (APC) (Ab190204) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (APC) (Ab180313).
Transferrin Receptor/CD71 Rat mAb (APC) (Ab190204) - Flow Cytometry Flow cytometry analysis of Transferrin Receptor/CD71 expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were stained with Transferrin Receptor/CD71 Rat mAb (APC) (Ab190204) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (APC) (Ab180313). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤0.3μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.
ZJ24F1113413
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